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The map label for this gene is sigE

Identifier: 218905035

GI number: 218905035

Start: 3756320

End: 3757039

Strand: Reverse

Name: sigE

Synonym: BCAH820_3918

Alternate gene names: 218905035

Gene position: 3757039-3756320 (Counterclockwise)

Preceding gene: 218905036

Following gene: 218905033

Centisome position: 70.85

GC content: 33.06

Gene sequence:

>720_bases
ATGATGAAATTAAAATTTTATTTAGTATACCTTTGGTATAAAGTATTGTTGAAATTAGGAATTAAGACCGATGAAATTTA
TTACATTGGTGGAAGTGAAGCGTTACCACCGCCGTTAACGAAAGAAGAAGAGGAAGTTTTATTAAATAAATTGCCAAAAG
GAGATCAGGCGGCAAGGTCGTTATTAATTGAACGCAATTTACGGCTAGTCGTATATATAGCAAGAAAGTTTGAAAATACA
GGGATAAATATTGAAGATTTGATTAGCATTGGAACAATCGGGCTTATTAAAGCGGTAAATACATTTAATCCAGAAAAGAA
AATAAAATTAGCAACATATGCCTCACGTTGTATCGAAAATGAAATCTTAATGCATTTACGCCGCAATAATAAAAATCGTT
CAGAAGTTTCTTTTGATGAACCACTTAACATCGATTGGGACGGGAATGAACTTTTATTATCAGATGTATTAGGGACAGAC
GATGATATTATTACAAAAGATTTAGAAGCTACTGTTGATCGTCATCTTCTAATGAAAGCATTACATCAATTGAATGATCG
TGAAAAACAAATTATGGAACTTCGGTTTGGGCTCGCTGGAGGAGAGGAAAAGACGCAAAAAGATGTGGCGGATATGCTTG
GGATTTCACAGTCATACATTTCGCGTTTGGAAAAAAGAATCATAAAAAGATTACGAAAAGAATTTAATAAAATGGTGTAA

Upstream 100 bases:

>100_bases
CAACAGTTGTCAACCAATGGAGAGTATCAATGTATTGTGCATCCAAAAATGTTGATTTCGCAAAAAATGGTAATTGCTTA
ATAATGGGAGGCGGGATAAT

Downstream 100 bases:

>100_bases
AGAATAAGACCAGCCGTTATGTAGGCTGGTCTTATTCTCTTTCGTTGTATGAGGAGAGACAACGAAAATGAAGAATGAAG
AGAAAACCGCATGCATAAAA

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MMKLKFYLVYLWYKVLLKLGIKTDEIYYIGGSEALPPPLTKEEEEVLLNKLPKGDQAARSLLIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMHLRRNNKNRSEVSFDEPLNIDWDGNELLLSDVLGTD
DDIITKDLEATVDRHLLMKALHQLNDREKQIMELRFGLAGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV

Sequences:

>Translated_239_residues
MMKLKFYLVYLWYKVLLKLGIKTDEIYYIGGSEALPPPLTKEEEEVLLNKLPKGDQAARSLLIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMHLRRNNKNRSEVSFDEPLNIDWDGNELLLSDVLGTD
DDIITKDLEATVDRHLLMKALHQLNDREKQIMELRFGLAGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV
>Mature_239_residues
MMKLKFYLVYLWYKVLLKLGIKTDEIYYIGGSEALPPPLTKEEEEVLLNKLPKGDQAARSLLIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMHLRRNNKNRSEVSFDEPLNIDWDGNELLLSDVLGTD
DDIITKDLEATVDRHLLMKALHQLNDREKQIMELRFGLAGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=28.90625, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789871, Length=103, Percent_Identity=42.7184466019417, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): RP35_BACAN (P62177)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_846283.1
- RefSeq:   YP_020685.1
- RefSeq:   YP_030006.1
- ProteinModelPortal:   P62177
- SMR:   P62177
- EnsemblBacteria:   EBBACT00000012093
- EnsemblBacteria:   EBBACT00000017203
- EnsemblBacteria:   EBBACT00000022798
- GeneID:   1086623
- GeneID:   2816772
- GeneID:   2850034
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4043
- KEGG:   bar:GBAA_4043
- KEGG:   bat:BAS3755
- TIGR:   BA_4043
- TIGR:   GBAA_4043
- GeneTree:   EBGT00070000031774
- HOGENOM:   HBG745096
- OMA:   RSMLIER
- ProtClustDB:   PRK08301
- BioCyc:   BANT260799:BAS3755-MONOMER
- BioCyc:   BANT261594:GBAA4043-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF000770
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02937
- TIGRFAMs:   TIGR02835

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 27637; Mature: 27637

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKLKFYLVYLWYKVLLKLGIKTDEIYYIGGSEALPPPLTKEEEEVLLNKLPKGDQAARS
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHH
LLIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EILMHLRRNNKNRSEVSFDEPLNIDWDGNELLLSDVLGTDDDIITKDLEATVDRHLLMKA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LHQLNDREKQIMELRFGLAGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV
HHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKLKFYLVYLWYKVLLKLGIKTDEIYYIGGSEALPPPLTKEEEEVLLNKLPKGDQAARS
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHH
LLIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EILMHLRRNNKNRSEVSFDEPLNIDWDGNELLLSDVLGTDDDIITKDLEATVDRHLLMKA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LHQLNDREKQIMELRFGLAGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMV
HHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629