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The map label for this gene is uraA [H]

Identifier: 218905020

GI number: 218905020

Start: 3743915

End: 3745198

Strand: Reverse

Name: uraA [H]

Synonym: BCAH820_3904

Alternate gene names: 218905020

Gene position: 3745198-3743915 (Counterclockwise)

Preceding gene: 218905021

Following gene: 218905019

Centisome position: 70.63

GC content: 39.88

Gene sequence:

>1284_bases
ATGGAACAAAAGCCAGTGTTAGACGTTAATGAAGTACCGAAACCGGGAAAATGGTTATTTTTAAGTATACAACATTTGTT
CGCGATGTTTGGATCAACAGTGCTTGTTCCGTTTTTAACAGGATTGAATCCGTCAGTAGCATTAATATCAAGTGGATTAG
GAACGTTAGCGTTTCTTCTTATAACGAAAGGTCAAGTACCTGCCTATCTTGGATCATCATTCGCCTTTATCGCACCGATT
ATAACAGCGAAAACGGCAGGGGGACCCGGAGCAGCAATGCTTGGTGGTTTGCTAGCAGGACTTGTATACATCTTAATCTC
ACTCGGAATTAAGAAATCAGGATCAAAGTGGATTATGAAATTACTACCACCAATCGTAGTTGGTCCAGTAGTAATGGTAA
TCGGGCTAGCTTTAGCACATACAGCAGTTAACATGGCGATGAATGGTGCGGATGGCAAATATAGCTTTACACACTTTTCA
GTAGCATTAGTAACATTAGCAATTACAATCATTTGCTCCATATTCGGGAGAGGGTTCTTCAGTATCATACCAGTTTTACT
TGGAATCATCGGAGGTTACATCTTCGCTTACTTCCAAGGGCTAGTAGACTTAAAGCCGGTAGCTGAGGCAAAATGGTTTG
TTGTACCAGATTTCACAGTACCATTTGTAACATACACACCAGAGTTTTCATGGAAGATTGTACTCTTGATGGTACCAGTT
GCACTAGTAACAATTTCAGAACATATCGGACATCAAATTGTATTAGGGAATGTTATTAAAAGAGATTTAATTGAAAAACC
AGGTTTACACCGTTCGATCTTCGGTGATGGAGTAGCAACATTAATCGCATCACTAATCGGTGGACCACCGAATACAACGT
ATGGTGAAAACATCGGCGTGCTCGCAATTACAAGAGCGTACAGTGTATACTTATTCATCGGTTCAGCGGTATTCGCAATC
ATGTTCGGATTTATTGGTAAGATTTCTGCACTGATTCATTCGATCCCAACACCAGTTATGGGTGGTGTATCAATCTTACT
ATTCGGTGTAATCGCATCAAGCGGATTACGCATGATGGTAGACGATAAAACAGATTTAAGTGACAAACGAAACTTAATGA
TTGCATCAGTAATACTAGTAATCGGTATTGGTGGAGCGGTACTTCACGTAGGAGAATCATTCCAAGTAGAAGGAATGGCA
CTAGCAGCAATTGTAGGTGTATTGTTAAATCTACTACTACCGGAAACGAAACAAATAAAACAATCTAACCAGATTGCTTC
ATAA

Upstream 100 bases:

>100_bases
TTCATATGTCAAATTTGGCATACGAAAGTGTAGGACTCTTTGTGCACAGGCACAAAGAGTTTTTTTATTGCAAAGATTTA
AACTGAAGGAGGATGTAACA

Downstream 100 bases:

>100_bases
TAATAACCTTTTAATTCAGTCCTGTGAGACCGGAAAGGGTGGCTTCTTTCTTGCACCCTAGTCTTACACGACTAGGGTAT
TTTTATGCAAAGGTTGTTAT

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALISSGLGTLAFLLITKGQVPAYLGSSFAFIAPI
ITAKTAGGPGAAMLGGLLAGLVYILISLGIKKSGSKWIMKLLPPIVVGPVVMVIGLALAHTAVNMAMNGADGKYSFTHFS
VALVTLAITIICSIFGRGFFSIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEFSWKIVLLMVPV
ALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGPPNTTYGENIGVLAITRAYSVYLFIGSAVFAI
MFGFIGKISALIHSIPTPVMGGVSILLFGVIASSGLRMMVDDKTDLSDKRNLMIASVILVIGIGGAVLHVGESFQVEGMA
LAAIVGVLLNLLLPETKQIKQSNQIAS

Sequences:

>Translated_427_residues
MEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALISSGLGTLAFLLITKGQVPAYLGSSFAFIAPI
ITAKTAGGPGAAMLGGLLAGLVYILISLGIKKSGSKWIMKLLPPIVVGPVVMVIGLALAHTAVNMAMNGADGKYSFTHFS
VALVTLAITIICSIFGRGFFSIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEFSWKIVLLMVPV
ALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGPPNTTYGENIGVLAITRAYSVYLFIGSAVFAI
MFGFIGKISALIHSIPTPVMGGVSILLFGVIASSGLRMMVDDKTDLSDKRNLMIASVILVIGIGGAVLHVGESFQVEGMA
LAAIVGVLLNLLLPETKQIKQSNQIAS
>Mature_427_residues
MEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALISSGLGTLAFLLITKGQVPAYLGSSFAFIAPI
ITAKTAGGPGAAMLGGLLAGLVYILISLGIKKSGSKWIMKLLPPIVVGPVVMVIGLALAHTAVNMAMNGADGKYSFTHFS
VALVTLAITIICSIFGRGFFSIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEFSWKIVLLMVPV
ALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGPPNTTYGENIGVLAITRAYSVYLFIGSAVFAI
MFGFIGKISALIHSIPTPVMGGVSILLFGVIASSGLRMMVDDKTDLSDKRNLMIASVILVIGIGGAVLHVGESFQVEGMA
LAAIVGVLLNLLLPETKQIKQSNQIAS

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=450, Percent_Identity=24.4444444444444, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40316845, Length=450, Percent_Identity=24.4444444444444, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI44680145, Length=445, Percent_Identity=24.0449438202247, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI44680143, Length=446, Percent_Identity=24.4394618834081, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788843, Length=412, Percent_Identity=45.6310679611651, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI87081818, Length=408, Percent_Identity=39.4607843137255, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082181, Length=440, Percent_Identity=25.9090909090909, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1790087, Length=450, Percent_Identity=24.4444444444444, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082178, Length=325, Percent_Identity=25.2307692307692, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17558856, Length=225, Percent_Identity=28, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17542262, Length=459, Percent_Identity=23.7472766884532, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356175, Length=448, Percent_Identity=25.4464285714286, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45073; Mature: 45073

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALISSGLGTLAFLL
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHH
ITKGQVPAYLGSSFAFIAPIITAKTAGGPGAAMLGGLLAGLVYILISLGIKKSGSKWIMK
HCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LLPPIVVGPVVMVIGLALAHTAVNMAMNGADGKYSFTHFSVALVTLAITIICSIFGRGFF
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHH
SIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEFSWKIVLLMVPV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHH
ALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGPPNTTYGENIGV
HHHHHHHHHCCEEHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCE
LAITRAYSVYLFIGSAVFAIMFGFIGKISALIHSIPTPVMGGVSILLFGVIASSGLRMMV
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEE
DDKTDLSDKRNLMIASVILVIGIGGAVLHVGESFQVEGMALAAIVGVLLNLLLPETKQIK
CCCCCCCHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
QSNQIAS
HHCCCCC
>Mature Secondary Structure
MEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALISSGLGTLAFLL
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHH
ITKGQVPAYLGSSFAFIAPIITAKTAGGPGAAMLGGLLAGLVYILISLGIKKSGSKWIMK
HCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LLPPIVVGPVVMVIGLALAHTAVNMAMNGADGKYSFTHFSVALVTLAITIICSIFGRGFF
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHH
SIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEFSWKIVLLMVPV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHH
ALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGPPNTTYGENIGV
HHHHHHHHHCCEEHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCE
LAITRAYSVYLFIGSAVFAIMFGFIGKISALIHSIPTPVMGGVSILLFGVIASSGLRMMV
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEE
DDKTDLSDKRNLMIASVILVIGIGGAVLHVGESFQVEGMALAAIVGVLLNLLLPETKQIK
CCCCCCCHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
QSNQIAS
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8206848 [H]