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The map label for this gene is pyrB [H]

Identifier: 218905019

GI number: 218905019

Start: 3742852

End: 3743766

Strand: Reverse

Name: pyrB [H]

Synonym: BCAH820_3903

Alternate gene names: 218905019

Gene position: 3743766-3742852 (Counterclockwise)

Preceding gene: 218905020

Following gene: 218905018

Centisome position: 70.6

GC content: 38.25

Gene sequence:

>915_bases
ATGAGCCATTTGTTAACGATGAGTGAATTATCGGAAGTAGAAATTTCAGAAATCCTAAAAGACGCAGAAGATTTTGCGAA
TGGGAAAGAGAGCAAAACGACAGAGCAAACTTTTGTTGCAAACTTGTTCTTTGAGAATAGTACGAGAACGAGATTTAGCT
TTGAAGTTGCTGAGAAGAGATTAGGACTTGATGTTTTAAACTTTTCAGCTGATGCATCTAGCGTACAAAAAGGAGAAACT
TTATACGATACGATAAGAACACTAGAATCAATCGGAACAAAAGCAGTGGTCATCCGCCATGAGCAAGATCGCTACTTCGA
TGAACTAAAAGATCAGGTGAATATTCCAATCTTAAACGCTGGAGATGGATGTGGAAATCATCCAACACAGTGCCTGCTCG
ATCTTCTTACCATTAAACAAGAGTTTGGAAGATTTGAAGGTTTGAAGATTGCGATAGTAGGAGATGTTCGTCATAGCCGA
GTAGCACGTTCTAATGCAGAAGCATTAACGAAGCTGGGTGCAACAATTTACTTTGCAAGTCCAGAAGAGTGGAAAGATGA
AGACAACACATTTGGAACATACAAACCATTAGATGAACTTGTTCCGGAAGTGGACGTAATGATGTTACTTCGTGTACAAC
ATGAGCGTCATGATCATTATGAAACAGACATTATGAAAGAGTATCATGAGAAACACGGATTAACAGTGGAAAGAGAAAAG
CGTATGAAAGAAGGAAGCATTATTATGCATCCAGCTCCTGTAAACCGAGATGTTGAAATTGCAAGTGAACTTGTTGAGTG
TGAGCGTTCACGCATATTTAAACAAATGGAAAATGGAGTTTACGTAAGAATGGCTGTACTAAAACGCGCCTTACCAAATG
TATTAGGAGGAATGAAACATGAATTATTTGTTTAA

Upstream 100 bases:

>100_bases
TCTTGCACCCTAGTCTTACACGACTAGGGTATTTTTATGCAAAGGTTGTTATAAAAATTCAAAAGTGGTAACATAAAAAT
CAGAAAGATGAGGGATGACG

Downstream 100 bases:

>100_bases
AAATGGTCGTTATATGAATGAAGAAGGAAAAATCGTAGCAACGGATCTTCTCGTACAAGACGGTAAAATCGCTAAAGTAG
CAGAAAATATTACGGCAGAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSHLLTMSELSEVEISEILKDAEDFANGKESKTTEQTFVANLFFENSTRTRFSFEVAEKRLGLDVLNFSADASSVQKGET
LYDTIRTLESIGTKAVVIRHEQDRYFDELKDQVNIPILNAGDGCGNHPTQCLLDLLTIKQEFGRFEGLKIAIVGDVRHSR
VARSNAEALTKLGATIYFASPEEWKDEDNTFGTYKPLDELVPEVDVMMLLRVQHERHDHYETDIMKEYHEKHGLTVEREK
RMKEGSIIMHPAPVNRDVEIASELVECERSRIFKQMENGVYVRMAVLKRALPNVLGGMKHELFV

Sequences:

>Translated_304_residues
MSHLLTMSELSEVEISEILKDAEDFANGKESKTTEQTFVANLFFENSTRTRFSFEVAEKRLGLDVLNFSADASSVQKGET
LYDTIRTLESIGTKAVVIRHEQDRYFDELKDQVNIPILNAGDGCGNHPTQCLLDLLTIKQEFGRFEGLKIAIVGDVRHSR
VARSNAEALTKLGATIYFASPEEWKDEDNTFGTYKPLDELVPEVDVMMLLRVQHERHDHYETDIMKEYHEKHGLTVEREK
RMKEGSIIMHPAPVNRDVEIASELVECERSRIFKQMENGVYVRMAVLKRALPNVLGGMKHELFV
>Mature_303_residues
SHLLTMSELSEVEISEILKDAEDFANGKESKTTEQTFVANLFFENSTRTRFSFEVAEKRLGLDVLNFSADASSVQKGETL
YDTIRTLESIGTKAVVIRHEQDRYFDELKDQVNIPILNAGDGCGNHPTQCLLDLLTIKQEFGRFEGLKIAIVGDVRHSRV
ARSNAEALTKLGATIYFASPEEWKDEDNTFGTYKPLDELVPEVDVMMLLRVQHERHDHYETDIMKEYHEKHGLTVEREKR
MKEGSIIMHPAPVNRDVEIASELVECERSRIFKQMENGVYVRMAVLKRALPNVLGGMKHELFV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=35.1973684210526, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI2367364, Length=305, Percent_Identity=34.7540983606557, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1790703, Length=188, Percent_Identity=28.7234042553192, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786469, Length=194, Percent_Identity=28.3505154639175, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=283, Percent_Identity=36.0424028268551, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=263, Percent_Identity=35.7414448669202, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24642586, Length=273, Percent_Identity=36.2637362637363, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34723; Mature: 34592

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLLTMSELSEVEISEILKDAEDFANGKESKTTEQTFVANLFFENSTRTRFSFEVAEKR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCEEHHHHHHHH
LGLDVLNFSADASSVQKGETLYDTIRTLESIGTKAVVIRHEQDRYFDELKDQVNIPILNA
HCCHHEECCCCHHHHHCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHCCCCEEEC
GDGCGNHPTQCLLDLLTIKQEFGRFEGLKIAIVGDVRHSRVARSNAEALTKLGATIYFAS
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCEEEECC
PEEWKDEDNTFGTYKPLDELVPEVDVMMLLRVQHERHDHYETDIMKEYHEKHGLTVEREK
CHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHH
RMKEGSIIMHPAPVNRDVEIASELVECERSRIFKQMENGVYVRMAVLKRALPNVLGGMKH
HHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCHH
ELFV
HHCC
>Mature Secondary Structure 
SHLLTMSELSEVEISEILKDAEDFANGKESKTTEQTFVANLFFENSTRTRFSFEVAEKR
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCEEHHHHHHHH
LGLDVLNFSADASSVQKGETLYDTIRTLESIGTKAVVIRHEQDRYFDELKDQVNIPILNA
HCCHHEECCCCHHHHHCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHCCCCEEEC
GDGCGNHPTQCLLDLLTIKQEFGRFEGLKIAIVGDVRHSRVARSNAEALTKLGATIYFAS
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCEEEECC
PEEWKDEDNTFGTYKPLDELVPEVDVMMLLRVQHERHDHYETDIMKEYHEKHGLTVEREK
CHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHH
RMKEGSIIMHPAPVNRDVEIASELVECERSRIFKQMENGVYVRMAVLKRALPNVLGGMKH
HHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCHH
ELFV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA