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The map label for this gene is rlmN

Identifier: 218904994

GI number: 218904994

Start: 3715033

End: 3716121

Strand: Reverse

Name: rlmN

Synonym: BCAH820_3878

Alternate gene names: 218904994

Gene position: 3716121-3715033 (Counterclockwise)

Preceding gene: 218904995

Following gene: 218904993

Centisome position: 70.08

GC content: 36.55

Gene sequence:

>1089_bases
GTGGAAACGACTGTGAGAAAACAAAAGAAAAACTTAGAAACGAAAAAACCATCAATTTATTCTTTACAACTTCATGAAAT
GCAAGATTGGTTAAAGGAGCAAGGAGAACCAAAGTTCCGTGCTGGACAAATTTTTGATTGGTTATATAAAAAACGTGTAA
AAAATTATGAGGATATGTCAAACCTTTCTAAAGGATTGCGTGAAAAATTGTCGAATTCCTTTGATATTACTACTTTAAAT
ACGTTAGTAAAACAAACGTCTTCGGATGGAACAATTAAATTCTTATTCCAATTATATGATGGATATTCTATTGAAACGGT
ATTAATGCGACATGAATATGGAAATTCCATTTGTGTAACAACACAAGTAGGTTGTCGTATTGGGTGTACGTTCTGTGCTT
CAACGCTTGGTGGTTTAAAGCGAAACTTAGAAGCTGGAGAAATTGTAGCGCAAGTAGTAGAAGTGCAACGTGCACTTGAT
GAATCAGAAGAACGTGTAAGTTCTCTTGTCGTTATGGGAATTGGAGAACCATTTGATAACTACGATAATTTAATGGGATT
CTTACGCATCATTAACCATGAAAAAGGACTTCATATTGGCGCGAGACATATGACAGTTTCGACAAGTGGAATCATTCCAA
AAATTTATAAGTTCGCTGAAGAAGACTTACAAATTAATTTTGCGATTTCACTGCATGCTCCAAACTCAGAATTACGTTCA
AAATTAATGCCGATTAACCGTGCTTATAAGCTACCTGATTTAATGGAAGCTATTAAATACTATGTAAATAGAACAGGGCG
TCGTATTACTTTTGAATATGGACTATTTGGAGGAGAAAATGACCAAGTTGAGCACGCTGAAGAACTTGCTGCGCTCTTAA
AAGGTGTAAAATGTCATGTGAACTTAATTCCGGTAAACTACGTACCTGAACGTGATTATGTACGTACGCCACGTGAACAA
ATTTTCCTATTTGAAAAAACGTTGAAAGATCGTGGAGTAAATGTAACGATTCGCCGTGAACAAGGTCATGATATTGATGC
AGCCTGCGGTCAGTTGCGTGCGAAGGAGCGCAAAGAAGAGACGAGGTGA

Upstream 100 bases:

>100_bases
CTAAGTCCGTATATGGATGAAGGTCAAGTACAAATTTTACCGCATTATTTTGCAACAGATGGCTTTTATATTGCTTGTTT
AAGGAAGAAGGTGTAGCATC

Downstream 100 bases:

>100_bases
CGAGATGAAAGCCGTGTTTCTATCAGATAAAGGAAAAGTTCGTCAACATAATGAAGATAGTGCAGGAGTTTTTCACAATT
TAGATGGAAATATTTTAGCG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFDITTLN
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEETR

Sequences:

>Translated_362_residues
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFDITTLN
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEETR
>Mature_362_residues
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFDITTLN
TLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALD
ESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVRTPREQ
IFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=352, Percent_Identity=35.7954545454545, Blast_Score=241, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_BACAA (C3P635)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868101.1
- ProteinModelPortal:   C3P635
- EnsemblBacteria:   EBBACT00000130060
- GeneID:   7853239
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4026
- GeneTree:   EBGT00050000001327
- ProtClustDB:   PRK14455
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41553; Mature: 41553

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMS
CCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
NLSKGLREKLSNSFDITTLNTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVT
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEE
TQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALDESEERVSSLVVMGIGEPFDN
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHEEEEECCCCCCC
YDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
HHHHHHHHHHHHCCCCCEECCEEEEEECCCCHHHHHHHHHCCEEEEEEEEEECCCHHHHH
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHV
HHCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEE
NLIPVNYVPERDYVRTPREQIFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEE
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
TR
CC
>Mature Secondary Structure
METTVRKQKKNLETKKPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMS
CCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHH
NLSKGLREKLSNSFDITTLNTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVT
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEE
TQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVEVQRALDESEERVSSLVVMGIGEPFDN
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHEEEEECCCCCCC
YDNLMGFLRIINHEKGLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRS
HHHHHHHHHHHHCCCCCEECCEEEEEECCCCHHHHHHHHHCCEEEEEEEEEECCCHHHHH
KLMPINRAYKLPDLMEAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHV
HHCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEE
NLIPVNYVPERDYVRTPREQIFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAKERKEE
EEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA