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The map label for this gene is appA [H]

Identifier: 218902114

GI number: 218902114

Start: 962446

End: 964032

Strand: Direct

Name: appA [H]

Synonym: BCAH820_0996

Alternate gene names: 218902114

Gene position: 962446-964032 (Clockwise)

Preceding gene: 218902112

Following gene: 218902115

Centisome position: 18.15

GC content: 35.29

Gene sequence:

>1587_bases
ATGAAGAAGAAGTTGTTTGTATTACCATTTGTCCTACTATTATTTATTGCATTAGCTGCTTGTTCAGGGGAAAAAGATTC
GAAGCAAGCAGGAACTAGTAAATCAGGGACTCCGAAAGATGGAGGAACGTTAACGATTGGTGTTAGCGATAACCCAGATA
CGATGAATCCGCTTTATGCGAATGATCGTGTGTCGTTAACGGTGCAGCAAGCTTTATATGCACCGCTGTATCATATGGAA
GACGGTAAGAAAAAGTTTGTTCTTGCTGAAAGCTTTACGCCTTCAGAGGATCAATTAACGTGGACGTTAAAATTAAAAGA
TAATTTGAAATGGCATGATGGTAAGAAAATTACATCAGACGACATAGCATTTACATTCCAATCTATTTTAGATGAGAAGC
AAAATAGCTCAAGTCGTGAAAACTTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAGTTCAA
TTCGTTTTACCACAAGTAAGCGCATCTTTTGAAGGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGAAGG
GGAGGCAGATTTAGCGAAGAGTAAGAAAAACTTACAACCTGTAGGATCAGGTCCGTTTAAGTTTAAAGAGTATAAGTCTG
ATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAGCTAAATTAGACTCTATCGTATACCGCGTTGTA
AAAGATCGTAATACAGCGAATGTTTCACTACAAAATGGTCAAATCAACATGAAGATGATTGAGCCGCAAGACTTTAAGAA
ATTAGACAGCACTGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTATTCTACTTATCTTATAACATGAATACAG
ATCTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCACATGCGTTAGATAAAAAAGAAATGATTAACTCAGCATTCGTT
TCTAGTCAATTTGCAGAACCAGCAAATTCAATTTTAACGCCAGACGCTATGTATTATGCAAAAGACATTAAAGATTATAA
GTATGATAAAAAAGAAGCAAAAGATTTATTAGCGAAAGCTGGCGTGAAAGATAAAGAAAAAGTACGCGTCATGTATGTAA
CGAATAATAAAATTATGGAAAGCTTAGCGCTTTATACACAACAAAAATTACAAGAAGTTGGCTTAGAAGTTGAACTGAAT
GCATTAGATGCTAGTGCAGCAAGTGACAAAAGCTTAGATAAAGAGAATAAAGAATATGACATTACATTCGGTGGTTACAT
AATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGTAATGCTGAATACAACTATGCACGTTATAAAAACGCTG
ATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGCGCAGAACTATATCATAAAATTCAAGAG
ACAGCTAGAGAAGACTTACCTTATCTACCAATTGCGTATCCGAAAGCAGTTATTGCAGTAGATAAAAAGTTTGATGGATT
GAAAGAAGCAAAAGCAATTCCAGTTACAATGTTTGAAGATTTATCTAAGATTTATGAAGTGAAATAA

Upstream 100 bases:

>100_bases
TGACAATACATACGGGTAGTGGTAATTTAAAACCTATAAGAAAACTCGGTATTAATTCCGAGTTTTCTTATAGGTGTAAA
TAGTTGAGAGGGGTCGAAAT

Downstream 100 bases:

>100_bases
GAAACTGTAAAGAAGCTGGTGGAAATCAGCTTCTTTATCAAGTTAAAGCCTAATTGATGTAGGCTAATGAAAGTTTCACT
TTACCTTGAGGGGGGAAGTT

Product: oligopeptide ABC transporter, oligopeptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGTPKDGGTLTIGVSDNPDTMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTVQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELN
ALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK

Sequences:

>Translated_528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGTPKDGGTLTIGVSDNPDTMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTVQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELN
ALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
>Mature_528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGTPKDGGTLTIGVSDNPDTMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTVQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELN
ALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=478, Percent_Identity=26.5690376569038, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1789966, Length=493, Percent_Identity=26.7748478701826, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787762, Length=529, Percent_Identity=26.2759924385633, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789887, Length=510, Percent_Identity=24.7058823529412, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1787551, Length=481, Percent_Identity=22.2453222453222, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1789397, Length=488, Percent_Identity=21.5163934426229, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787495, Length=441, Percent_Identity=24.0362811791383, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081878, Length=472, Percent_Identity=21.6101694915254, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59797; Mature: 59797

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGTPKDGGTLTIGVSDNPDTMNPLYA
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
NDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSD
CCEEEEEEHHHHHCCHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCH
DIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTVQFVLPQVSASFEGVMNDFFPI
HHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHEEEEEEHHHHHHHHHHHHHCCCC
PKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
CHHHHCCCHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEECHHHHCCCHHHHHHHHHHHH
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKE
HCCCCCEEEEECCEEEEEEECCHHHHHHCCCCCEEEEEECCCEEEEEEECCCCHHHHHHH
VRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
GVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELNALDASAASDKSLDKENKEYD
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEE
ITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
EEECCEEECCCCCCCHHHEECCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKLFVLPFVLLLFIALAACSGEKDSKQAGTSKSGTPKDGGTLTIGVSDNPDTMNPLYA
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
NDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSD
CCEEEEEEHHHHHCCHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCH
DIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTVQFVLPQVSASFEGVMNDFFPI
HHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHEEEEEEHHHHHHHHHHHHHCCCC
PKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
CHHHHCCCHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEECHHHHCCCHHHHHHHHHHHH
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKE
HCCCCCEEEEECCEEEEEEECCHHHHHHCCCCCEEEEEECCCEEEEEEECCCCHHHHHHH
VRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
GVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELNALDASAASDKSLDKENKEYD
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEE
ITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
EEECCEEECCCCCCCHHHEECCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
TAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]