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The map label for this gene is pyk [H]

Identifier: 218884554

GI number: 218884554

Start: 1163600

End: 1165024

Strand: Direct

Name: pyk [H]

Synonym: DKAM_1243

Alternate gene names: 218884554

Gene position: 1163600-1165024 (Clockwise)

Preceding gene: 218884553

Following gene: 218884561

Centisome position: 85.23

GC content: 44.14

Gene sequence:

>1425_bases
GTGTTGGGTAGGGTGAAAATAATAGCGACCCTTGGACCCAGTATTCGTGATTACAATGTAATTAAGAAAATGATCAAGGA
GGGTGTAGCCGGGTTTAGGATCAACTGTGCTCATGGTGATGAGAATACTTGGATAGAATATGTTAAACTAGTTAGAGACG
CGTCCAGCGATCTCGGCAAAGCTATTCCACTAATACTGGATACACCGGGTCCCCAGGTTAGAAGCGGTGATTTCCAGGAG
TTCACTGTAAAGCAAGGCGATAACGTTGTAATTTCATCGAACCCGGGTAATGGTGGTGGAGAGAAAAAGATAATTATTGT
TCCAGCCAAGGAGTTTTACACAGCCGCTTCAACCGGGGACATTATACTCTATGGGGACGGCGAGATATCATTCAGAGTAA
TTAACACCGGTGAGGACTTCGTAGAGGTGGTAGTGTTAAACGATGGGGTTGTGAAACCCAGGAAGAAGGTTGTTATAGAG
GGGAAGGAGTACCCGGTGGCGTTTCCCAGTGAGGTGGATAGAAGAGTATTTAAGTTCGCTAGCGATGTAAAAGCGACCTT
CGTTGCTCTAAGCTATGTTAGGAGCCTGAAGGATGTGGAATTGGCGAAGGATGTATTATCCAGGCATGGATGGATGCCCG
GCTTCATAGCAAAGATAGAGACGAGGAGCGGATTCATAAATATGGGTGAGATAATCGAGGTGGTTGATGGAGTAATAGTT
GCAAGAGGAGATCTAGGGCTTCACTTCCCTCTCGAGAAACTACCATTGATACAGAAGGAGATAGTAAGGGAGGCAATTAA
ACATGGTAAGACCGTGATTATTGCAACAGAGATATTGGAGTCCATGGTGAATAATCCAAGGCCTAGTAGGAGTGATGTAG
TAGACCTATATAATTCCGTGGAAGATCTCGTCGACGCAGTGTTATTCACTAATGAGACAGCTATTGGGAAATACCCTGTT
GAAGTAGTTAGATGGACTAGGAGAATTATTGAGGCAGCCGAGTCCGAGCTTTCCAGGGCACTAATATCCGAGTATAGAGG
CGCAATCAAAGCTATGAATCTCCTGGATAAATACGTGCAGGGACTCGTACTACTAGCTGAGAGCCTCGGAGGCACTATAG
TAGGTTATAGTAAGACTGGTAGAATGCCTACACTAGTCTCAAAATATAGGCCGCAGATCAAAGTCTATATAGGCGTAAGC
AACCGGTTCCTGGCTGAAAAGCTATCGATAAAGTATGGATTGGAAATCACTGATATGTCCCAGGTCATCCAGGACAACCT
TGAAAAGGAGTATGAGGTGGGCGTTGAAAAACTGGTTGAGACACTAGTTAAACAGAATTATCTGAGACCTGGCGATATAG
TTGTTGAGACATATGCAAAGCCTCAGACAAGTATACATGAAATAAGGATTAGACAAATAATTTAG

Upstream 100 bases:

>100_bases
CTGGCTAAAGAAATAAGCGATTCAACAAGTAAGGCCAGAGAACACGGGCTTGTTAAGGGGGTTAAACTAATAGATGTAAT
TAACAAGCTGTAGGTGCCTC

Downstream 100 bases:

>100_bases
AACTTATGAAGAAGCCGCCGGCGGGATTTGAACCCGCGTCCACCGGCTTTCCAACGACTCCGGTTAAATAAGCCGATACG
AGGCCGGCGCTCCACCGGGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLGRVKIIATLGPSIRDYNVIKKMIKEGVAGFRINCAHGDENTWIEYVKLVRDASSDLGKAIPLILDTPGPQVRSGDFQE
FTVKQGDNVVISSNPGNGGGEKKIIIVPAKEFYTAASTGDIILYGDGEISFRVINTGEDFVEVVVLNDGVVKPRKKVVIE
GKEYPVAFPSEVDRRVFKFASDVKATFVALSYVRSLKDVELAKDVLSRHGWMPGFIAKIETRSGFINMGEIIEVVDGVIV
ARGDLGLHFPLEKLPLIQKEIVREAIKHGKTVIIATEILESMVNNPRPSRSDVVDLYNSVEDLVDAVLFTNETAIGKYPV
EVVRWTRRIIEAAESELSRALISEYRGAIKAMNLLDKYVQGLVLLAESLGGTIVGYSKTGRMPTLVSKYRPQIKVYIGVS
NRFLAEKLSIKYGLEITDMSQVIQDNLEKEYEVGVEKLVETLVKQNYLRPGDIVVETYAKPQTSIHEIRIRQII

Sequences:

>Translated_474_residues
MLGRVKIIATLGPSIRDYNVIKKMIKEGVAGFRINCAHGDENTWIEYVKLVRDASSDLGKAIPLILDTPGPQVRSGDFQE
FTVKQGDNVVISSNPGNGGGEKKIIIVPAKEFYTAASTGDIILYGDGEISFRVINTGEDFVEVVVLNDGVVKPRKKVVIE
GKEYPVAFPSEVDRRVFKFASDVKATFVALSYVRSLKDVELAKDVLSRHGWMPGFIAKIETRSGFINMGEIIEVVDGVIV
ARGDLGLHFPLEKLPLIQKEIVREAIKHGKTVIIATEILESMVNNPRPSRSDVVDLYNSVEDLVDAVLFTNETAIGKYPV
EVVRWTRRIIEAAESELSRALISEYRGAIKAMNLLDKYVQGLVLLAESLGGTIVGYSKTGRMPTLVSKYRPQIKVYIGVS
NRFLAEKLSIKYGLEITDMSQVIQDNLEKEYEVGVEKLVETLVKQNYLRPGDIVVETYAKPQTSIHEIRIRQII
>Mature_474_residues
MLGRVKIIATLGPSIRDYNVIKKMIKEGVAGFRINCAHGDENTWIEYVKLVRDASSDLGKAIPLILDTPGPQVRSGDFQE
FTVKQGDNVVISSNPGNGGGEKKIIIVPAKEFYTAASTGDIILYGDGEISFRVINTGEDFVEVVVLNDGVVKPRKKVVIE
GKEYPVAFPSEVDRRVFKFASDVKATFVALSYVRSLKDVELAKDVLSRHGWMPGFIAKIETRSGFINMGEIIEVVDGVIV
ARGDLGLHFPLEKLPLIQKEIVREAIKHGKTVIIATEILESMVNNPRPSRSDVVDLYNSVEDLVDAVLFTNETAIGKYPV
EVVRWTRRIIEAAESELSRALISEYRGAIKAMNLLDKYVQGLVLLAESLGGTIVGYSKTGRMPTLVSKYRPQIKVYIGVS
NRFLAEKLSIKYGLEITDMSQVIQDNLEKEYEVGVEKLVETLVKQNYLRPGDIVVETYAKPQTSIHEIRIRQII

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=464, Percent_Identity=29.9568965517241, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI33286420, Length=464, Percent_Identity=29.9568965517241, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI33286418, Length=464, Percent_Identity=29.9568965517241, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI32967597, Length=471, Percent_Identity=31.2101910828025, Blast_Score=195, Evalue=9e-50,
Organism=Homo sapiens, GI10835121, Length=471, Percent_Identity=31.2101910828025, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=33.5689045936396, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=33.5689045936396, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI310128736, Length=230, Percent_Identity=30.8695652173913, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310128734, Length=230, Percent_Identity=30.8695652173913, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310128738, Length=213, Percent_Identity=30.0469483568075, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1787965, Length=418, Percent_Identity=30.3827751196172, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=28.0082987551867, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17544584, Length=423, Percent_Identity=30.2600472813239, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71984413, Length=337, Percent_Identity=32.9376854599407, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17506829, Length=337, Percent_Identity=32.9376854599407, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71984406, Length=347, Percent_Identity=32.8530259365994, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17506831, Length=347, Percent_Identity=32.8530259365994, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319279, Length=411, Percent_Identity=31.1435523114355, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6324923, Length=407, Percent_Identity=29.97542997543, Blast_Score=163, Evalue=6e-41,
Organism=Drosophila melanogaster, GI28571814, Length=467, Percent_Identity=31.2633832976445, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24648964, Length=467, Percent_Identity=31.2633832976445, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24581235, Length=404, Percent_Identity=30.1980198019802, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24648966, Length=318, Percent_Identity=33.9622641509434, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24646914, Length=242, Percent_Identity=33.0578512396694, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52685; Mature: 52685

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRVKIIATLGPSIRDYNVIKKMIKEGVAGFRINCAHGDENTWIEYVKLVRDASSDLGK
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
AIPLILDTPGPQVRSGDFQEFTVKQGDNVVISSNPGNGGGEKKIIIVPAKEFYTAASTGD
CCCEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCEEEEEEECHHHHHCCCCCC
IILYGDGEISFRVINTGEDFVEVVVLNDGVVKPRKKVVIEGKEYPVAFPSEVDRRVFKFA
EEEEECCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
SDVKATFVALSYVRSLKDVELAKDVLSRHGWMPGFIAKIETRSGFINMGEIIEVVDGVIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCHHHHHHHHCCEEE
ARGDLGLHFPLEKLPLIQKEIVREAIKHGKTVIIATEILESMVNNPRPSRSDVVDLYNSV
EECCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHH
EDLVDAVLFTNETAIGKYPVEVVRWTRRIIEAAESELSRALISEYRGAIKAMNLLDKYVQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVLLAESLGGTIVGYSKTGRMPTLVSKYRPQIKVYIGVSNRFLAEKLSIKYGLEITDMS
HHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEHHHH
QVIQDNLEKEYEVGVEKLVETLVKQNYLRPGDIVVETYAKPQTSIHEIRIRQII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLGRVKIIATLGPSIRDYNVIKKMIKEGVAGFRINCAHGDENTWIEYVKLVRDASSDLGK
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
AIPLILDTPGPQVRSGDFQEFTVKQGDNVVISSNPGNGGGEKKIIIVPAKEFYTAASTGD
CCCEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCEEEEEEECHHHHHCCCCCC
IILYGDGEISFRVINTGEDFVEVVVLNDGVVKPRKKVVIEGKEYPVAFPSEVDRRVFKFA
EEEEECCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
SDVKATFVALSYVRSLKDVELAKDVLSRHGWMPGFIAKIETRSGFINMGEIIEVVDGVIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCHHHHHHHHCCEEE
ARGDLGLHFPLEKLPLIQKEIVREAIKHGKTVIIATEILESMVNNPRPSRSDVVDLYNSV
EECCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHH
EDLVDAVLFTNETAIGKYPVEVVRWTRRIIEAAESELSRALISEYRGAIKAMNLLDKYVQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVLLAESLGGTIVGYSKTGRMPTLVSKYRPQIKVYIGVSNRFLAEKLSIKYGLEITDMS
HHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEHHHH
QVIQDNLEKEYEVGVEKLVETLVKQNYLRPGDIVVETYAKPQTSIHEIRIRQII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]