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The map label for this gene is pcm [H]

Identifier: 218884378

GI number: 218884378

Start: 1011771

End: 1012454

Strand: Direct

Name: pcm [H]

Synonym: DKAM_1067

Alternate gene names: 218884378

Gene position: 1011771-1012454 (Clockwise)

Preceding gene: 218884377

Following gene: 218884384

Centisome position: 74.11

GC content: 47.22

Gene sequence:

>684_bases
TTGTCCGGTTTAGAAGAACAAAAACGACTCATTATTGAAGAACTACACTCACTAGGCTATCTGAGAAGCGAGAAAGTCAT
AAAAGCCCTTCTCAAAGTCCCCAGGGAGCTCTTTCTACCTCCACATCTAAGGGAGTACGCCTATGTTGATACACCTCTCC
CAATAGGTTTCGGTCAAACCATAAGCGCGATACATATGGTAGCCATAATGACAGAGGAGTTGGACCCTGCCCCAGGCGAT
AGGGTTCTCGAGGTGGGAACTGGTTCAGGTTATCAGGCAGCTGTACTGGCAGAGATAGTTGCAAGGAGTGGTGAGGGGAA
AAAAGGACATGTATACACTATAGAAAGGATCCCGGAGCTGGCCGAGTATGCTAGAAAGCGCCTTGAGGAAGCTGGTTATA
GCAGTGATGTCACGGTGATAGTGGGCGATGGAACACTGGGGCTACCTGAGAAAGCACCATTCGATAAAATAATAGTCACA
GCCGCCGCCCCCTATGTACCGAACCCATTAATAGAGCAGCTGGCTGAAGGAGGAAGATTAGTCATACCCGTAGGCGATGT
ATATCTCCAACGACTACTCACAGTAGAGAAGTACGGGAGTTCGATAACCAAGAGGTATGGAATAGAATGTGTGTTCGTAC
CGTTAATTGGTAAGTATGGATGGCATACCGATTCAACATATTGA

Upstream 100 bases:

>100_bases
TGGGATGTCCCCTAGCATATACAATCTCTGCTTCAACCATTATGCTTAAACCCCGCTATTAAGAATATTTTAACTATGAA
AATTAAATAGGGGTTACGAT

Downstream 100 bases:

>100_bases
AGCCTTAATGAAGCAGGATTATCAACAATTTTCAGAGTTGAGGGTTTTATAGGTCTTTATATGCCCACGGTGAAGCGTGA
GCAGAGGCACAACCGGTCCC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSGLEEQKRLIIEELHSLGYLRSEKVIKALLKVPRELFLPPHLREYAYVDTPLPIGFGQTISAIHMVAIMTEELDPAPGD
RVLEVGTGSGYQAAVLAEIVARSGEGKKGHVYTIERIPELAEYARKRLEEAGYSSDVTVIVGDGTLGLPEKAPFDKIIVT
AAAPYVPNPLIEQLAEGGRLVIPVGDVYLQRLLTVEKYGSSITKRYGIECVFVPLIGKYGWHTDSTY

Sequences:

>Translated_227_residues
MSGLEEQKRLIIEELHSLGYLRSEKVIKALLKVPRELFLPPHLREYAYVDTPLPIGFGQTISAIHMVAIMTEELDPAPGD
RVLEVGTGSGYQAAVLAEIVARSGEGKKGHVYTIERIPELAEYARKRLEEAGYSSDVTVIVGDGTLGLPEKAPFDKIIVT
AAAPYVPNPLIEQLAEGGRLVIPVGDVYLQRLLTVEKYGSSITKRYGIECVFVPLIGKYGWHTDSTY
>Mature_226_residues
SGLEEQKRLIIEELHSLGYLRSEKVIKALLKVPRELFLPPHLREYAYVDTPLPIGFGQTISAIHMVAIMTEELDPAPGDR
VLEVGTGSGYQAAVLAEIVARSGEGKKGHVYTIERIPELAEYARKRLEEAGYSSDVTVIVGDGTLGLPEKAPFDKIIVTA
AAPYVPNPLIEQLAEGGRLVIPVGDVYLQRLLTVEKYGSSITKRYGIECVFVPLIGKYGWHTDSTY

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=218, Percent_Identity=36.697247706422, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1789100, Length=206, Percent_Identity=43.6893203883495, Blast_Score=144, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71983477, Length=215, Percent_Identity=34.8837209302326, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI193207222, Length=213, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17981723, Length=215, Percent_Identity=34.8837209302326, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24965; Mature: 24834

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLEEQKRLIIEELHSLGYLRSEKVIKALLKVPRELFLPPHLREYAYVDTPLPIGFGQT
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCHHHHEEECCCCCCCCCHH
ISAIHMVAIMTEELDPAPGDRVLEVGTGSGYQAAVLAEIVARSGEGKKGHVYTIERIPEL
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHH
AEYARKRLEEAGYSSDVTVIVGDGTLGLPEKAPFDKIIVTAAAPYVPNPLIEQLAEGGRL
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCEE
VIPVGDVYLQRLLTVEKYGSSITKRYGIECVFVPLIGKYGWHTDSTY
EEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SGLEEQKRLIIEELHSLGYLRSEKVIKALLKVPRELFLPPHLREYAYVDTPLPIGFGQT
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCHHHHEEECCCCCCCCCHH
ISAIHMVAIMTEELDPAPGDRVLEVGTGSGYQAAVLAEIVARSGEGKKGHVYTIERIPEL
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHH
AEYARKRLEEAGYSSDVTVIVGDGTLGLPEKAPFDKIIVTAAAPYVPNPLIEQLAEGGRL
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCEE
VIPVGDVYLQRLLTVEKYGSSITKRYGIECVFVPLIGKYGWHTDSTY
EEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA