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The map label for this gene is prf1 [H]

Identifier: 218884171

GI number: 218884171

Start: 825967

End: 827058

Strand: Reverse

Name: prf1 [H]

Synonym: DKAM_0860

Alternate gene names: 218884171

Gene position: 827058-825967 (Counterclockwise)

Preceding gene: 218884173

Following gene: 218884170

Centisome position: 60.58

GC content: 46.34

Gene sequence:

>1092_bases
ATGAGAGTGGATAAGCAAAGGTTAAGGGAGATAATTAAGGAGTTGAAGAAGTGGAAGGCCCATGCAACGATATTGCTGAG
CCTATATGTGCCGCCGGGTAGACCTATAGGTGATGTATTAAACCTGCTTCGTCAGGAGCTCTCGGTAGCCGATAATATAA
AGCTTAAGAAGACAAGGAACGCTGTTGAGAGAGCGTTGTCGATAGCCATTGAGAGGATGAGCAAGATACCCAAGGTCCCT
GATAAAGGACTAGTATTATTCGCTGGAGAAGATCCTGATACAGGGAAATCGATAACAGTTATGTTGATCCCACCAGAGGA
GGTGCCGGTATTCTTCTATAGGACGGATAAGGTATTCCATACAGAGTTCCTCGAGGAGATGGTCAGCGAATCCAATATAA
TTGGCTTACTAATAGTGGAGCGTGATGCCGCTACAATCGGCCTGCTTAAGGGTAATAGGCTGCAGGTAGTTGAGGAGCTA
GAGGACTATATACCAGGCAAGCATCAGAAAGGTGGTCAGAGCCAGCGTAGATACGATAGAATAATTGAGCAAATGGTTGA
AGACTTCTATAAGAGGGTTGGTGAACACGCTAACAACGTATTCCTACCACTACTAGAGCAAGGCAAGCTTAAGGCCCTCC
TAGTTGGCGGACCAGCCTATGCTAAATACGATTTCATGGAGAAGGATTACCTGGATTACAGGCTTAAGAAGATAGTGCTG
CCAGAGTTCATCGATGTAGCCTACCAGGGTGAACCAGGCTTAAGGGAAATGATAATGAGAGCCGGGGACCTATTAAAGGA
GCAAGAATATGCTGAAACACTTAAGGCAATCGAGGAGTTCAAGTATCACTTGGCAAAAGACGACGGGTTAATAGTGTACG
GTGAAGAAGAAGTGCGCAACGCTCTTGAAATGGGTCTTGTAGGCATCCTGCTAATAAGTGAGCAACGGGAGGATATAGAG
GAGTGGCTTGAGCTAGCAAGAAAGCGAGGAGCTAAACCACTAGTCGTAAGCACCGATATACCTGAAGGCGAGTGGTTCGC
ACAAACCTTCAATGGGTTGGCCGGAATACTGAGAACCCGTGTATCATGGTAG

Upstream 100 bases:

>100_bases
ATGGGTTTACCTAGATATATTTAAACCCCAAAATATATACCATGTTATCCCGGTGTAAAAGGGATGAGCCTTTAGCTATA
GTGAAGACCAAATAGAATCC

Downstream 100 bases:

>100_bases
CTTCCAACCGGTCAGCTAGTTTAATTTTTAATTAGTAGAAATTATAAGCCTGTAGGAACAATATTAATTATGGTGGGGGA
TTAGGAAATGATAGATCCCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRVDKQRLREIIKELKKWKAHATILLSLYVPPGRPIGDVLNLLRQELSVADNIKLKKTRNAVERALSIAIERMSKIPKVP
DKGLVLFAGEDPDTGKSITVMLIPPEEVPVFFYRTDKVFHTEFLEEMVSESNIIGLLIVERDAATIGLLKGNRLQVVEEL
EDYIPGKHQKGGQSQRRYDRIIEQMVEDFYKRVGEHANNVFLPLLEQGKLKALLVGGPAYAKYDFMEKDYLDYRLKKIVL
PEFIDVAYQGEPGLREMIMRAGDLLKEQEYAETLKAIEEFKYHLAKDDGLIVYGEEEVRNALEMGLVGILLISEQREDIE
EWLELARKRGAKPLVVSTDIPEGEWFAQTFNGLAGILRTRVSW

Sequences:

>Translated_363_residues
MRVDKQRLREIIKELKKWKAHATILLSLYVPPGRPIGDVLNLLRQELSVADNIKLKKTRNAVERALSIAIERMSKIPKVP
DKGLVLFAGEDPDTGKSITVMLIPPEEVPVFFYRTDKVFHTEFLEEMVSESNIIGLLIVERDAATIGLLKGNRLQVVEEL
EDYIPGKHQKGGQSQRRYDRIIEQMVEDFYKRVGEHANNVFLPLLEQGKLKALLVGGPAYAKYDFMEKDYLDYRLKKIVL
PEFIDVAYQGEPGLREMIMRAGDLLKEQEYAETLKAIEEFKYHLAKDDGLIVYGEEEVRNALEMGLVGILLISEQREDIE
EWLELARKRGAKPLVVSTDIPEGEWFAQTFNGLAGILRTRVSW
>Mature_363_residues
MRVDKQRLREIIKELKKWKAHATILLSLYVPPGRPIGDVLNLLRQELSVADNIKLKKTRNAVERALSIAIERMSKIPKVP
DKGLVLFAGEDPDTGKSITVMLIPPEEVPVFFYRTDKVFHTEFLEEMVSESNIIGLLIVERDAATIGLLKGNRLQVVEEL
EDYIPGKHQKGGQSQRRYDRIIEQMVEDFYKRVGEHANNVFLPLLEQGKLKALLVGGPAYAKYDFMEKDYLDYRLKKIVL
PEFIDVAYQGEPGLREMIMRAGDLLKEQEYAETLKAIEEFKYHLAKDDGLIVYGEEEVRNALEMGLVGILLISEQREDIE
EWLELARKRGAKPLVVSTDIPEGEWFAQTFNGLAGILRTRVSW

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=406, Percent_Identity=26.8472906403941, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI72000368, Length=408, Percent_Identity=25.9803921568627, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319619, Length=404, Percent_Identity=24.7524752475248, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28574632, Length=407, Percent_Identity=26.2899262899263, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21356433, Length=407, Percent_Identity=26.2899262899263, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667392, Length=407, Percent_Identity=26.2899262899263, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667388, Length=407, Percent_Identity=26.2899262899263, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667380, Length=407, Percent_Identity=26.2899262899263, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24667384, Length=407, Percent_Identity=26.2899262899263, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 41580; Mature: 41580

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDKQRLREIIKELKKWKAHATILLSLYVPPGRPIGDVLNLLRQELSVADNIKLKKTRN
CCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
AVERALSIAIERMSKIPKVPDKGLVLFAGEDPDTGKSITVMLIPPEEVPVFFYRTDKVFH
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCHHHH
TEFLEEMVSESNIIGLLIVERDAATIGLLKGNRLQVVEELEDYIPGKHQKGGQSQRRYDR
HHHHHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
IIEQMVEDFYKRVGEHANNVFLPLLEQGKLKALLVGGPAYAKYDFMEKDYLDYRLKKIVL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
PEFIDVAYQGEPGLREMIMRAGDLLKEQEYAETLKAIEEFKYHLAKDDGLIVYGEEEVRN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHH
ALEMGLVGILLISEQREDIEEWLELARKRGAKPLVVSTDIPEGEWFAQTFNGLAGILRTR
HHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
VSW
CCC
>Mature Secondary Structure
MRVDKQRLREIIKELKKWKAHATILLSLYVPPGRPIGDVLNLLRQELSVADNIKLKKTRN
CCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
AVERALSIAIERMSKIPKVPDKGLVLFAGEDPDTGKSITVMLIPPEEVPVFFYRTDKVFH
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCHHHH
TEFLEEMVSESNIIGLLIVERDAATIGLLKGNRLQVVEELEDYIPGKHQKGGQSQRRYDR
HHHHHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
IIEQMVEDFYKRVGEHANNVFLPLLEQGKLKALLVGGPAYAKYDFMEKDYLDYRLKKIVL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
PEFIDVAYQGEPGLREMIMRAGDLLKEQEYAETLKAIEEFKYHLAKDDGLIVYGEEEVRN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHH
ALEMGLVGILLISEQREDIEEWLELARKRGAKPLVVSTDIPEGEWFAQTFNGLAGILRTR
HHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
VSW
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]