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The map label for this gene is gatD [H]

Identifier: 218884130

GI number: 218884130

Start: 787061

End: 788416

Strand: Direct

Name: gatD [H]

Synonym: DKAM_0819

Alternate gene names: 218884130

Gene position: 787061-788416 (Clockwise)

Preceding gene: 218884127

Following gene: 218884131

Centisome position: 57.65

GC content: 47.79

Gene sequence:

>1356_bases
ATGGAGCTGTATTATGGTTATAGTGGTAATGTAGCTAGGATACTTAGAAGTATAGGGGTTGAACCAGGTGATAAAATAGT
CGTAGTGTTGAAGGATGGAACTGTTTTCAACGGGATCCTCTTGCCCCGTCAGGTCCTCTATTCCGGGAGGCCTGTGCTGG
TCTTAAAACTAGATAACGGCTATAATATAGGTTTAAACATCGACGAGGTAAGCGATTTAAGGCTGGTTTCGAAGAAACAG
GTTCATCACCATGTAGGTGTCGAAGCCGTTGGGAGGGCTGAGGGATTACCCCGTATAGTATTACTGGGCACGGGAGGTAC
GATCGCATCCAAAGTTGATTATGAGACGGGAGCAGTTACACCGGTCCTCACCCCTGGCGAGATACTTGAATGGGTACCGG
AGCTGGCTGAAATAGCTGTCTTTAACGCTAGGGAGGTAATGAGTATTTTCAGCGAGGATATAGAGCCACTTCACTGGGAG
AACCTATCCCGGGAGGTATATAAGGAGCTCGTTGAAGGAGTTGATGGCGTTGTAATAGCCCATGGTACGGACATGATGTC
TTACTCTGCTTCAGCCCTAGCATTCTCAATAGTGAATAAGCCTGCTCCAATAGTGTTTGTTGGCTCCCAGAGGAGTAGTG
ATAGGCCGAGCAGTGACTCAGCATTTAATCTAAGAGCCGCATTCATAGCTGCCTCCAAGGGGTCCTTCGCTGAATCCGTA
ATCGTAATGCATGGTGAAACATCCGATACATACGCGCTGGTACATAGAGGCGTGAAAGCCAGGAAGATGCATACTAGTAG
AAGGGATGCATTTCAATCAATAAACGATAAGCCGATAGCCAAGGTGTATCCTGAGACAATGGAGGTACGGATCGTGGGAA
GAATCATTGAGCACCGTGGTAAAAACAAGGAGGCACAGTTAAAGAATAAGTTTGATGACAGGGTAGCACTGGTTAAAGTC
TACCCGGGCCTCCAACCAGAGGTGCTTGAGACGTTGATAGATAAGGGCTTCCATGGAATAGTCCTAGAGGGAAGCGGGCT
GGGACACATGCCTAACAAGCTCATCCCAGTTATAGCGAGGGCTGTTGAAAACGATATACCCGTGGTAATGACTAGTCAAT
GCCTTTTCGGCCGCGTCAACCTCTACGTCTATAGTACTGGTAGAAGACTACTCGAAGCCGGCGTGATCCCGGGCTCTGAT
ATACTTCCCGAGACAGCTTATGTAAAGCTCTCATGGATCCTTGGCAGCGTATCAAGGGACCTTGAGGAAGTTAGGAAAGT
TTTCCTCACGAATCTCGCTGGAGAATTCAATGAGAGGCATATACTAGACCTCTACCCGAGGTGGAATCATGAGTAG

Upstream 100 bases:

>100_bases
AATCCATTGTAAGTGTGAGCCCCCTTAATGATGCAGGTATATCATGCTACTTATTGATATTTAAGTAGAATAGGTTCTAT
ATTTTCTAACCGGTGATTGA

Downstream 100 bases:

>100_bases
GATCAACTATAGTGAAACCGGGTTAAAGGTCGGCCTCGAGATACATGTCCAATTAGATACGGCGAGGAAGCTCTTCTGTA
AATGCCCAACCCTCCTCAGT

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-Aspartate; ammonia [C]

Alternate protein names: Glu-ADT subunit D [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MELYYGYSGNVARILRSIGVEPGDKIVVVLKDGTVFNGILLPRQVLYSGRPVLVLKLDNGYNIGLNIDEVSDLRLVSKKQ
VHHHVGVEAVGRAEGLPRIVLLGTGGTIASKVDYETGAVTPVLTPGEILEWVPELAEIAVFNAREVMSIFSEDIEPLHWE
NLSREVYKELVEGVDGVVIAHGTDMMSYSASALAFSIVNKPAPIVFVGSQRSSDRPSSDSAFNLRAAFIAASKGSFAESV
IVMHGETSDTYALVHRGVKARKMHTSRRDAFQSINDKPIAKVYPETMEVRIVGRIIEHRGKNKEAQLKNKFDDRVALVKV
YPGLQPEVLETLIDKGFHGIVLEGSGLGHMPNKLIPVIARAVENDIPVVMTSQCLFGRVNLYVYSTGRRLLEAGVIPGSD
ILPETAYVKLSWILGSVSRDLEEVRKVFLTNLAGEFNERHILDLYPRWNHE

Sequences:

>Translated_451_residues
MELYYGYSGNVARILRSIGVEPGDKIVVVLKDGTVFNGILLPRQVLYSGRPVLVLKLDNGYNIGLNIDEVSDLRLVSKKQ
VHHHVGVEAVGRAEGLPRIVLLGTGGTIASKVDYETGAVTPVLTPGEILEWVPELAEIAVFNAREVMSIFSEDIEPLHWE
NLSREVYKELVEGVDGVVIAHGTDMMSYSASALAFSIVNKPAPIVFVGSQRSSDRPSSDSAFNLRAAFIAASKGSFAESV
IVMHGETSDTYALVHRGVKARKMHTSRRDAFQSINDKPIAKVYPETMEVRIVGRIIEHRGKNKEAQLKNKFDDRVALVKV
YPGLQPEVLETLIDKGFHGIVLEGSGLGHMPNKLIPVIARAVENDIPVVMTSQCLFGRVNLYVYSTGRRLLEAGVIPGSD
ILPETAYVKLSWILGSVSRDLEEVRKVFLTNLAGEFNERHILDLYPRWNHE
>Mature_451_residues
MELYYGYSGNVARILRSIGVEPGDKIVVVLKDGTVFNGILLPRQVLYSGRPVLVLKLDNGYNIGLNIDEVSDLRLVSKKQ
VHHHVGVEAVGRAEGLPRIVLLGTGGTIASKVDYETGAVTPVLTPGEILEWVPELAEIAVFNAREVMSIFSEDIEPLHWE
NLSREVYKELVEGVDGVVIAHGTDMMSYSASALAFSIVNKPAPIVFVGSQRSSDRPSSDSAFNLRAAFIAASKGSFAESV
IVMHGETSDTYALVHRGVKARKMHTSRRDAFQSINDKPIAKVYPETMEVRIVGRIIEHRGKNKEAQLKNKFDDRVALVKV
YPGLQPEVLETLIDKGFHGIVLEGSGLGHMPNKLIPVIARAVENDIPVVMTSQCLFGRVNLYVYSTGRRLLEAGVIPGSD
ILPETAYVKLSWILGSVSRDLEEVRKVFLTNLAGEFNERHILDLYPRWNHE

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=302, Percent_Identity=28.8079470198675, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788065, Length=306, Percent_Identity=29.0849673202614, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789327, Length=348, Percent_Identity=27.8735632183908, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI193207372, Length=399, Percent_Identity=28.0701754385965, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI193207374, Length=399, Percent_Identity=28.0701754385965, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17558280, Length=399, Percent_Identity=28.0701754385965, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17558278, Length=399, Percent_Identity=28.0701754385965, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320527, Length=351, Percent_Identity=27.0655270655271, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323189, Length=266, Percent_Identity=29.3233082706767, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323187, Length=266, Percent_Identity=29.3233082706767, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323186, Length=266, Percent_Identity=29.3233082706767, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323184, Length=266, Percent_Identity=29.3233082706767, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24645530, Length=286, Percent_Identity=31.4685314685315, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24639609, Length=281, Percent_Identity=28.1138790035587, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: 3.5.1.1 [C]

Molecular weight: Translated: 49774; Mature: 49774

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELYYGYSGNVARILRSIGVEPGDKIVVVLKDGTVFNGILLPRQVLYSGRPVLVLKLDNG
CEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHCCCCEEEEEECCC
YNIGLNIDEVSDLRLVSKKQVHHHVGVEAVGRAEGLPRIVLLGTGGTIASKVDYETGAVT
EEECEECCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCC
PVLTPGEILEWVPELAEIAVFNAREVMSIFSEDIEPLHWENLSREVYKELVEGVDGVVIA
EEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEE
HGTDMMSYSASALAFSIVNKPAPIVFVGSQRSSDRPSSDSAFNLRAAFIAASKGSFAESV
ECCCHHHCCHHHHEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEE
IVMHGETSDTYALVHRGVKARKMHTSRRDAFQSINDKPIAKVYPETMEVRIVGRIIEHRG
EEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHCC
KNKEAQLKNKFDDRVALVKVYPGLQPEVLETLIDKGFHGIVLEGSGLGHMPNKLIPVIAR
CCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
AVENDIPVVMTSQCLFGRVNLYVYSTGRRLLEAGVIPGSDILPETAYVKLSWILGSVSRD
HHCCCCCEEEECCCEEEEEEEEEEECCHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHH
LEEVRKVFLTNLAGEFNERHILDLYPRWNHE
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MELYYGYSGNVARILRSIGVEPGDKIVVVLKDGTVFNGILLPRQVLYSGRPVLVLKLDNG
CEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHCCCCEEEEEECCC
YNIGLNIDEVSDLRLVSKKQVHHHVGVEAVGRAEGLPRIVLLGTGGTIASKVDYETGAVT
EEECEECCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCC
PVLTPGEILEWVPELAEIAVFNAREVMSIFSEDIEPLHWENLSREVYKELVEGVDGVVIA
EEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEE
HGTDMMSYSASALAFSIVNKPAPIVFVGSQRSSDRPSSDSAFNLRAAFIAASKGSFAESV
ECCCHHHCCHHHHEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEE
IVMHGETSDTYALVHRGVKARKMHTSRRDAFQSINDKPIAKVYPETMEVRIVGRIIEHRG
EEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHCC
KNKEAQLKNKFDDRVALVKVYPGLQPEVLETLIDKGFHGIVLEGSGLGHMPNKLIPVIAR
CCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
AVENDIPVVMTSQCLFGRVNLYVYSTGRRLLEAGVIPGSDILPETAYVKLSWILGSVSRD
HHCCCCCEEEECCCEEEEEEEEEEECCHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHH
LEEVRKVFLTNLAGEFNERHILDLYPRWNHE
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Asparagine; H2O [C]

Specific reaction: L-Asparagine + H2O --> L-Aspartate + ammonia [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA