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The map label for this gene is trm1 [H]

Identifier: 218884122

GI number: 218884122

Start: 779649

End: 780839

Strand: Reverse

Name: trm1 [H]

Synonym: DKAM_0811

Alternate gene names: 218884122

Gene position: 780839-779649 (Counterclockwise)

Preceding gene: 218884123

Following gene: 218884121

Centisome position: 57.19

GC content: 45.09

Gene sequence:

>1191_bases
ATGACTGAAGTAAAGATACTCAGCCGTGAACAATTGATCCAGGAGGGATTAGCTAAGCTCGTAATACCCCGGATGGATGA
GTATAGAAGACCCGATGGATCAATAGAGCCGGCTTGGATGCCTGTCTTCTATAATCCACAAGCCATAGTAAGCAGGGATG
TGACCGTCTCAGTCCTCAGGACCCTGTATGGTTCGAGGAATATATTTTTCATTGATCCATTAGCCGGCACTGGAATACGT
AGCATTCGCATAGCGCTCGAGAAGGGTGGTGAGGGCATAGTAAACGATATTGATCCACATGCATTATACTACATAAGGAA
AAACCTGCGGCTCAACAACCTATACCCTGGAAAACTACACGTGTACTCGCAGGAGGCAAACGTCCTTCTCAATAATTTTA
CCTTCACGGGTATATCACTCGACTATATAGACCTAGATCCCTACGGCTCCCCAGTCCCATTCGTGGACTCGGTGTTTAAA
CCCCTGGGTAAGAGGGCGTTGATAGGTGTCACGGCAACCGATACAGCCCCGCTCACATGTAGTCACTACAGGAAAACCCT
TAGGAGATACTGGTTTACTTGTGTCCACACCGATTTCGAGAAAGAAATAGGGGCTAGGTTACTCATCGCGTACATAGTTA
AACACGCTTCAGCACTCGACATTGCTGCTAGGCCATTGCTTATATTCATTTATAAGCATTATTACAGGGTTTTCTTTGAA
ACCCTGAGGAGCACGGGGGAAGCATATCGATTAATTAATGAATGCCTGGGTTATATATGGTACTGCCCATCGACACTGGA
AAGAGGATATATTAAATCCCTGGATGAAGCCAGGGATTTAACATGTATTAATAACGAGAAACCGGTTCTAACAGGCGAAA
CATGGATCTGCAGCCTAGGGGAGGCTGAGTTCATTGATAAGGTATTCAATGAAGCCGATAAAATAAGCTGGCTTCACAAG
GATTCGAGGAGAATACTTGGGCTCCTCAGGAATGAGACTAGGTTGAATAACCCGTATGTACGAATAGATAAGCTATGCTC
TATTATTGGGAGAAATATGCCGAAATACGAGACCCTCATAGAGGCTTTGAAGCAACACGGCATCATGGCTTCCCGCACAC
ACTTTGATCCACGGGGATTAAAGATAGATAGTGATGTGAAGGCCTTGACCGAGGTGTTGAAGAGTATTTAA

Upstream 100 bases:

>100_bases
AGGGGTTGATTAATAAAGTAATCGAGGCCCTGGCTCCGTCTCGATCCTGAGGGAGGATCTCAACCGTGGAGATATGGGGA
CAAACGGTGGTGTATAGTCA

Downstream 100 bases:

>100_bases
TCCGGTGGTTAACATGTAGCAAGATTTTTTACTCTTCACTCTTACTTAAACTATAATTGGGGATACTCTTGTCTCAATTA
TTCGATAAAATCCGTGGAAT

Product: N2,N2-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTEVKILSREQLIQEGLAKLVIPRMDEYRRPDGSIEPAWMPVFYNPQAIVSRDVTVSVLRTLYGSRNIFFIDPLAGTGIR
SIRIALEKGGEGIVNDIDPHALYYIRKNLRLNNLYPGKLHVYSQEANVLLNNFTFTGISLDYIDLDPYGSPVPFVDSVFK
PLGKRALIGVTATDTAPLTCSHYRKTLRRYWFTCVHTDFEKEIGARLLIAYIVKHASALDIAARPLLIFIYKHYYRVFFE
TLRSTGEAYRLINECLGYIWYCPSTLERGYIKSLDEARDLTCINNEKPVLTGETWICSLGEAEFIDKVFNEADKISWLHK
DSRRILGLLRNETRLNNPYVRIDKLCSIIGRNMPKYETLIEALKQHGIMASRTHFDPRGLKIDSDVKALTEVLKSI

Sequences:

>Translated_396_residues
MTEVKILSREQLIQEGLAKLVIPRMDEYRRPDGSIEPAWMPVFYNPQAIVSRDVTVSVLRTLYGSRNIFFIDPLAGTGIR
SIRIALEKGGEGIVNDIDPHALYYIRKNLRLNNLYPGKLHVYSQEANVLLNNFTFTGISLDYIDLDPYGSPVPFVDSVFK
PLGKRALIGVTATDTAPLTCSHYRKTLRRYWFTCVHTDFEKEIGARLLIAYIVKHASALDIAARPLLIFIYKHYYRVFFE
TLRSTGEAYRLINECLGYIWYCPSTLERGYIKSLDEARDLTCINNEKPVLTGETWICSLGEAEFIDKVFNEADKISWLHK
DSRRILGLLRNETRLNNPYVRIDKLCSIIGRNMPKYETLIEALKQHGIMASRTHFDPRGLKIDSDVKALTEVLKSI
>Mature_395_residues
TEVKILSREQLIQEGLAKLVIPRMDEYRRPDGSIEPAWMPVFYNPQAIVSRDVTVSVLRTLYGSRNIFFIDPLAGTGIRS
IRIALEKGGEGIVNDIDPHALYYIRKNLRLNNLYPGKLHVYSQEANVLLNNFTFTGISLDYIDLDPYGSPVPFVDSVFKP
LGKRALIGVTATDTAPLTCSHYRKTLRRYWFTCVHTDFEKEIGARLLIAYIVKHASALDIAARPLLIFIYKHYYRVFFET
LRSTGEAYRLINECLGYIWYCPSTLERGYIKSLDEARDLTCINNEKPVLTGETWICSLGEAEFIDKVFNEADKISWLHKD
SRRILGLLRNETRLNNPYVRIDKLCSIIGRNMPKYETLIEALKQHGIMASRTHFDPRGLKIDSDVKALTEVLKSI

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups [H]

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family [H]

Homologues:

Organism=Homo sapiens, GI8923219, Length=353, Percent_Identity=25.7790368271955, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI209862871, Length=353, Percent_Identity=25.7790368271955, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI216548445, Length=336, Percent_Identity=26.4880952380952, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI163792194, Length=197, Percent_Identity=25.8883248730964, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320325, Length=463, Percent_Identity=23.5421166306695, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921190, Length=354, Percent_Identity=24.0112994350282, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022923
- InterPro:   IPR002905 [H]

Pfam domain/function: PF02005 TRM [H]

EC number: =2.1.1.32 [H]

Molecular weight: Translated: 45406; Mature: 45275

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVKILSREQLIQEGLAKLVIPRMDEYRRPDGSIEPAWMPVFYNPQAIVSRDVTVSVLR
CCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEECCHHHHHCCHHHHHHH
TLYGSRNIFFIDPLAGTGIRSIRIALEKGGEGIVNDIDPHALYYIRKNLRLNNLYPGKLH
HHHCCCCEEEECCCCCCCHHEEEEEEECCCCCCCCCCCCHHHHHHHHCCEECCCCCCEEE
VYSQEANVLLNNFTFTGISLDYIDLDPYGSPVPFVDSVFKPLGKRALIGVTATDTAPLTC
EEECCCCEEEECEEEECEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCH
SHYRKTLRRYWFTCVHTDFEKEIGARLLIAYIVKHASALDIAARPLLIFIYKHYYRVFFE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRSTGEAYRLINECLGYIWYCPSTLERGYIKSLDEARDLTCINNEKPVLTGETWICSLG
HHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEECCCEEEECC
EAEFIDKVFNEADKISWLHKDSRRILGLLRNETRLNNPYVRIDKLCSIIGRNMPKYETLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
EALKQHGIMASRTHFDPRGLKIDSDVKALTEVLKSI
HHHHHCCCEEECCCCCCCCCEECCHHHHHHHHHHCC
>Mature Secondary Structure 
TEVKILSREQLIQEGLAKLVIPRMDEYRRPDGSIEPAWMPVFYNPQAIVSRDVTVSVLR
CCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEECCHHHHHCCHHHHHHH
TLYGSRNIFFIDPLAGTGIRSIRIALEKGGEGIVNDIDPHALYYIRKNLRLNNLYPGKLH
HHHCCCCEEEECCCCCCCHHEEEEEEECCCCCCCCCCCCHHHHHHHHCCEECCCCCCEEE
VYSQEANVLLNNFTFTGISLDYIDLDPYGSPVPFVDSVFKPLGKRALIGVTATDTAPLTC
EEECCCCEEEECEEEECEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCH
SHYRKTLRRYWFTCVHTDFEKEIGARLLIAYIVKHASALDIAARPLLIFIYKHYYRVFFE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRSTGEAYRLINECLGYIWYCPSTLERGYIKSLDEARDLTCINNEKPVLTGETWICSLG
HHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEECCCEEEECC
EAEFIDKVFNEADKISWLHKDSRRILGLLRNETRLNNPYVRIDKLCSIIGRNMPKYETLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
EALKQHGIMASRTHFDPRGLKIDSDVKALTEVLKSI
HHHHHCCCEEECCCCCCCCCEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA