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The map label for this gene is thiI

Identifier: 218884002

GI number: 218884002

Start: 661750

End: 663198

Strand: Reverse

Name: thiI

Synonym: DKAM_0691

Alternate gene names: 218884002

Gene position: 663198-661750 (Counterclockwise)

Preceding gene: 218884006

Following gene: 218883999

Centisome position: 48.58

GC content: 49.83

Gene sequence:

>1449_bases
TTGCCGACATACCTTATTACAGTAGCTGGAGAAATACCTTTGAAATCCAAGAAGACCAGGTCGAGGCTCTACTACAGGCT
CATCGATAATATAAGGAGGAGGCTTGCCCGGAGAAACATCACTCTGCAAGTAGCCAAGGTAATTGATGCCAAGATACTTG
TTGAGACACAGGTCGAGGCTCTACAGGAGTTGAGCAGGGTATTCGGGGTCCATAGAGTATCGGAGGTCCAGGTGCTGGAA
TTCAGGGATCTTGGTGAGCTAGCCAAGGAGATAGCGTCTAGGACGATAGAGCATGTAAGGGATAGGAAGTTTGCTGTGAG
GGTGAAGAGGAGTGGAAGACACGGATTCACATCCCTCGACGTAGCCAGGGAGGTGGGAGCATTACTGAAACCGTATTCGA
AGGGCGTGGACCTGGAAAACCCGGATATAGAGGTAGAGGTTGAGGTCAGGGGTAATAAGGCATACTTATACAGCAATGTC
GCTATGGGTCCCGGAGGTCTTCCACTCGGATCAAGTGGGAGAGCACTAGTCTTGTTTTCAGGTGGCTTTGACTCTCCTGT
AGCAGCGTGGATGATTGCTAAGAGGGGGGTGGAGGTCGACTTCCTACACTATGTAATGGGCTCCAGCGAGGTCTCTCGCC
AGGCATTCAGCGTGGCAAGGAAGCTATCCGAGGAGTGGCTCAGCAGCTATAACCCGAGGTTTATAACAGTCGATTTTACA
CCTCTTATAGCCGAGATAGAGGAGAGAATAGAGTGGAGTTATAGGCAGGTGGTTCTCAGGGCATTAATGTACATGGTTGC
CGACAAGATCGCTACGGAGCTAGGATACAACACCATCGTAACCGGTGAGGCCCTTTCACAGGCATCCAGCCAGACGCTTG
CCAACCTGGTTGCGGTAGAGAGTGCTGTTTCACCGAGGAGCATTATATTGAGGCCTCTAATAGGCTTTGATAAAGAGGAG
ATCATAGAGTATTCGAGAAGGATCGGGCTATACGACTACTCTTCCAGGGTTGCCGAGACGTGTGCAATAGCTCCAACACA
CGTGGTTACCAGGATTTCCAGTGAGAAGCTTAAATCATTAATTGAGAGGCTGGACGTGAGGCTTGTGGAGAGGATGGCGG
GAGAGTACAGGGTGGTAGATGTATTCTCTGCAAGTCCTGAGGAGGCTGTACCAGGCTATAGTGAGGAAATAGATTCGATA
CCAGGCGATTCAATAATCATAGATGTCAGGAGTTATGAGGAGTATAAGAGGGATGCGTTACCGGGAGCAATTCATCTAAG
CATGGTTGACTTCAACAATCTACCCAGAGATAAGCCGGTAGTACTTTATTGTACAACAGGGGGGATCAGCTTACTCTTAG
CGAGGGAGCTCCGCGGTAAGGGGTTTAAGGCGTATAGCCTACGTGGGGGCTTAGCAAGATATAGGGCTGGCTTGGAAAAG
ACGCGGTGA

Upstream 100 bases:

>100_bases
AGAAATGGGGAAACGGCATCAACCAGCACCAACCCATATCAGGACTAAACCCCCCTATGAAACATTATCGGAATAGTGGC
ATGGGAAGGGTTGAGAAGAA

Downstream 100 bases:

>100_bases
TTCTATTTGCCAACCCTGCGCTTCTTAATATAGTTCAACACATCCTTGGCTCGGGGGCCAGCCAGCCACTCCACAACTTC
ACTGGGTAGTCTATAGCTCT

Product: Thiamine biosynthesis/tRNA modification protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MPTYLITVAGEIPLKSKKTRSRLYYRLIDNIRRRLARRNITLQVAKVIDAKILVETQVEALQELSRVFGVHRVSEVQVLE
FRDLGELAKEIASRTIEHVRDRKFAVRVKRSGRHGFTSLDVAREVGALLKPYSKGVDLENPDIEVEVEVRGNKAYLYSNV
AMGPGGLPLGSSGRALVLFSGGFDSPVAAWMIAKRGVEVDFLHYVMGSSEVSRQAFSVARKLSEEWLSSYNPRFITVDFT
PLIAEIEERIEWSYRQVVLRALMYMVADKIATELGYNTIVTGEALSQASSQTLANLVAVESAVSPRSIILRPLIGFDKEE
IIEYSRRIGLYDYSSRVAETCAIAPTHVVTRISSEKLKSLIERLDVRLVERMAGEYRVVDVFSASPEEAVPGYSEEIDSI
PGDSIIIDVRSYEEYKRDALPGAIHLSMVDFNNLPRDKPVVLYCTTGGISLLLARELRGKGFKAYSLRGGLARYRAGLEK
TR

Sequences:

>Translated_482_residues
MPTYLITVAGEIPLKSKKTRSRLYYRLIDNIRRRLARRNITLQVAKVIDAKILVETQVEALQELSRVFGVHRVSEVQVLE
FRDLGELAKEIASRTIEHVRDRKFAVRVKRSGRHGFTSLDVAREVGALLKPYSKGVDLENPDIEVEVEVRGNKAYLYSNV
AMGPGGLPLGSSGRALVLFSGGFDSPVAAWMIAKRGVEVDFLHYVMGSSEVSRQAFSVARKLSEEWLSSYNPRFITVDFT
PLIAEIEERIEWSYRQVVLRALMYMVADKIATELGYNTIVTGEALSQASSQTLANLVAVESAVSPRSIILRPLIGFDKEE
IIEYSRRIGLYDYSSRVAETCAIAPTHVVTRISSEKLKSLIERLDVRLVERMAGEYRVVDVFSASPEEAVPGYSEEIDSI
PGDSIIIDVRSYEEYKRDALPGAIHLSMVDFNNLPRDKPVVLYCTTGGISLLLARELRGKGFKAYSLRGGLARYRAGLEK
TR
>Mature_481_residues
PTYLITVAGEIPLKSKKTRSRLYYRLIDNIRRRLARRNITLQVAKVIDAKILVETQVEALQELSRVFGVHRVSEVQVLEF
RDLGELAKEIASRTIEHVRDRKFAVRVKRSGRHGFTSLDVAREVGALLKPYSKGVDLENPDIEVEVEVRGNKAYLYSNVA
MGPGGLPLGSSGRALVLFSGGFDSPVAAWMIAKRGVEVDFLHYVMGSSEVSRQAFSVARKLSEEWLSSYNPRFITVDFTP
LIAEIEERIEWSYRQVVLRALMYMVADKIATELGYNTIVTGEALSQASSQTLANLVAVESAVSPRSIILRPLIGFDKEEI
IEYSRRIGLYDYSSRVAETCAIAPTHVVTRISSEKLKSLIERLDVRLVERMAGEYRVVDVFSASPEEAVPGYSEEIDSIP
GDSIIIDVRSYEEYKRDALPGAIHLSMVDFNNLPRDKPVVLYCTTGGISLLLARELRGKGFKAYSLRGGLARYRAGLEKT
R

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=475, Percent_Identity=31.7894736842105, Blast_Score=230, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_DESK1 (B8D4I6)

Other databases:

- EMBL:   CP001140
- RefSeq:   YP_002428384.1
- ProteinModelPortal:   B8D4I6
- GeneID:   7170870
- GenomeReviews:   CP001140_GR
- KEGG:   dka:DKAM_0691
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00450
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF00581 Rhodanese; PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 53986; Mature: 53855

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 444-444 BINDING 260-260 BINDING 282-282 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTYLITVAGEIPLKSKKTRSRLYYRLIDNIRRRLARRNITLQVAKVIDAKILVETQVEA
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
LQELSRVFGVHRVSEVQVLEFRDLGELAKEIASRTIEHVRDRKFAVRVKRSGRHGFTSLD
HHHHHHHHCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
VAREVGALLKPYSKGVDLENPDIEVEVEVRGNKAYLYSNVAMGPGGLPLGSSGRALVLFS
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCEEEEEEECCCCCCCCCCCCCCCEEEEEE
GGFDSPVAAWMIAKRGVEVDFLHYVMGSSEVSRQAFSVARKLSEEWLSSYNPRFITVDFT
CCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECH
PLIAEIEERIEWSYRQVVLRALMYMVADKIATELGYNTIVTGEALSQASSQTLANLVAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHH
SAVSPRSIILRPLIGFDKEEIIEYSRRIGLYDYSSRVAETCAIAPTHVVTRISSEKLKSL
HCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHHH
IERLDVRLVERMAGEYRVVDVFSASPEEAVPGYSEEIDSIPGDSIIIDVRSYEEYKRDAL
HHHHHHHHHHHHCCCEEEEEEECCCCHHCCCCCHHHHHCCCCCEEEEEEHHHHHHHHHCC
PGAIHLSMVDFNNLPRDKPVVLYCTTGGISLLLARELRGKGFKAYSLRGGLARYRAGLEK
CCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCC
TR
CC
>Mature Secondary Structure 
PTYLITVAGEIPLKSKKTRSRLYYRLIDNIRRRLARRNITLQVAKVIDAKILVETQVEA
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
LQELSRVFGVHRVSEVQVLEFRDLGELAKEIASRTIEHVRDRKFAVRVKRSGRHGFTSLD
HHHHHHHHCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
VAREVGALLKPYSKGVDLENPDIEVEVEVRGNKAYLYSNVAMGPGGLPLGSSGRALVLFS
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCEEEEEEECCCCCCCCCCCCCCCEEEEEE
GGFDSPVAAWMIAKRGVEVDFLHYVMGSSEVSRQAFSVARKLSEEWLSSYNPRFITVDFT
CCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECH
PLIAEIEERIEWSYRQVVLRALMYMVADKIATELGYNTIVTGEALSQASSQTLANLVAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHH
SAVSPRSIILRPLIGFDKEEIIEYSRRIGLYDYSSRVAETCAIAPTHVVTRISSEKLKSL
HCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHHH
IERLDVRLVERMAGEYRVVDVFSASPEEAVPGYSEEIDSIPGDSIIIDVRSYEEYKRDAL
HHHHHHHHHHHHCCCEEEEEEECCCCHHCCCCCHHHHHCCCCCEEEEEEHHHHHHHHHCC
PGAIHLSMVDFNNLPRDKPVVLYCTTGGISLLLARELRGKGFKAYSLRGGLARYRAGLEK
CCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCC
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA