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The map label for this gene is rfcL [H]

Identifier: 218884001

GI number: 218884001

Start: 660467

End: 661747

Strand: Direct

Name: rfcL [H]

Synonym: DKAM_0690

Alternate gene names: 218884001

Gene position: 660467-661747 (Clockwise)

Preceding gene: 218884000

Following gene: 218884003

Centisome position: 48.38

GC content: 47.78

Gene sequence:

>1281_bases
TTGAGCCAGGAGAGAGTACCGTGGATAATAAAATATAGGCCCAAGACCCTTGACGAGGTAGTAAACCAGGATGAAGCCAA
GTCAAAGCTACTGGAATGGCTCAGCTCATGGGAGAAGGGGAAGCCTAGTAAGAAGGCAGCATTACTTCATGGTCCTCCAG
GCTGCGGCAAGACGAGCCTTGTAGAGGCCCTAGCCAGGAGTAAGGGCTACCAGTTACTGGAGATGAATGCCAGTGACGCA
AGGCGGAAGGAGGATATCGAGAGAATAGTTAAATTAGCAAGTCGCTCGGGAGCATTAACCGGGTCTAGGAAGATAATACT
GCTTGATGAAGTCGATGGGATGGATGTTAGGGCGGATGCCGGTGGCGTAGAAGCCCTGGTGGAGGTAATCAAGGTTTCTG
CTAACCCGATAATAATGACGGCTAATAACCCGTATTCACAGATGCTACGCCCCCTTAGAGAGCTCTCAGAGATGATAGCC
TTCAAGAGACTTACTCCAAGAGACGTTGTAACAGTGTTGAAGAGGATATGTAGTGCTGAGAAACTGGTATGCGAGGATCA
AGCCCTCGATGAGATAGCTAAGAGGAGTGAGGGCGACTTAAGAAGCGCTATAAATGATTTAGAAGCTATGGCTGGAGCCA
GTGAAAGAATCACTCTTGGGCTAGTGAAATCATTCTCAACATACAGGGATAGAACCTATGCACCGTACGAGGCATTACAG
AAACTCTTCAACTCCAGGTATATATTCCAGGCTAGGGAAGCCCCAACCTCAACTGATTTAACCCCCGACGAGTTCATGGT
ATGGATTAACGAGCATATACCAACATACTATGAGGACCTAGAGGAGATCGTGAGAGCCTATGATGCATTAAGCAAGGCTG
ACGTATACATGGGAAGGATAATTAAAACCAATAACTGGGATCTATTAACCTACGCATTAGACATGATGGGACCTGGTGTT
GCATTCGCGAGGAAGACATATAGGTATAGGTGGAAGCCCTTCAAGCAACCCGAGAGAATCAAGCTGATGAGCGAGACAAA
GAAGTCCAGGGAGATCCGGGAAGCCCTAGCCGAGCAATTAGCCAGCCACATACTTGCCAGTAAGGCTGTAGTGAAGAGTG
ATGTAATACCGTTTCTAAGGGTGATTTTCAGGAGCAATCCAAGATATGCTGCTAAACTTGCCGAGAGCTATAGACTACCC
AGTGAAGTTGTGGAGTGGCTGGCTGGCCCCCGAGCCAAGGATGTGTTGAACTATATTAAGAAGCGCAGGGTTGGCAAATA
G

Upstream 100 bases:

>100_bases
ACAGTTCAGGCTTGTCGAGGGAGCCGATGACGAGATACAGTTGAACGCGTTTCTAGCCAGGCTAGCTTTAATAGGTAAGA
AATTCAAGACGGGGTAGCCC

Downstream 100 bases:

>100_bases
AATCACCGCGTCTTTTCCAAGCCAGCCCTATATCTTGCTAAGCCCCCACGTAGGCTATACGCCTTAAACCCCTTACCGCG
GAGCTCCCTCGCTAAGAGTA

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSQERVPWIIKYRPKTLDEVVNQDEAKSKLLEWLSSWEKGKPSKKAALLHGPPGCGKTSLVEALARSKGYQLLEMNASDA
RRKEDIERIVKLASRSGALTGSRKIILLDEVDGMDVRADAGGVEALVEVIKVSANPIIMTANNPYSQMLRPLRELSEMIA
FKRLTPRDVVTVLKRICSAEKLVCEDQALDEIAKRSEGDLRSAINDLEAMAGASERITLGLVKSFSTYRDRTYAPYEALQ
KLFNSRYIFQAREAPTSTDLTPDEFMVWINEHIPTYYEDLEEIVRAYDALSKADVYMGRIIKTNNWDLLTYALDMMGPGV
AFARKTYRYRWKPFKQPERIKLMSETKKSREIREALAEQLASHILASKAVVKSDVIPFLRVIFRSNPRYAAKLAESYRLP
SEVVEWLAGPRAKDVLNYIKKRRVGK

Sequences:

>Translated_426_residues
MSQERVPWIIKYRPKTLDEVVNQDEAKSKLLEWLSSWEKGKPSKKAALLHGPPGCGKTSLVEALARSKGYQLLEMNASDA
RRKEDIERIVKLASRSGALTGSRKIILLDEVDGMDVRADAGGVEALVEVIKVSANPIIMTANNPYSQMLRPLRELSEMIA
FKRLTPRDVVTVLKRICSAEKLVCEDQALDEIAKRSEGDLRSAINDLEAMAGASERITLGLVKSFSTYRDRTYAPYEALQ
KLFNSRYIFQAREAPTSTDLTPDEFMVWINEHIPTYYEDLEEIVRAYDALSKADVYMGRIIKTNNWDLLTYALDMMGPGV
AFARKTYRYRWKPFKQPERIKLMSETKKSREIREALAEQLASHILASKAVVKSDVIPFLRVIFRSNPRYAAKLAESYRLP
SEVVEWLAGPRAKDVLNYIKKRRVGK
>Mature_425_residues
SQERVPWIIKYRPKTLDEVVNQDEAKSKLLEWLSSWEKGKPSKKAALLHGPPGCGKTSLVEALARSKGYQLLEMNASDAR
RKEDIERIVKLASRSGALTGSRKIILLDEVDGMDVRADAGGVEALVEVIKVSANPIIMTANNPYSQMLRPLRELSEMIAF
KRLTPRDVVTVLKRICSAEKLVCEDQALDEIAKRSEGDLRSAINDLEAMAGASERITLGLVKSFSTYRDRTYAPYEALQK
LFNSRYIFQAREAPTSTDLTPDEFMVWINEHIPTYYEDLEEIVRAYDALSKADVYMGRIIKTNNWDLLTYALDMMGPGVA
FARKTYRYRWKPFKQPERIKLMSETKKSREIREALAEQLASHILASKAVVKSDVIPFLRVIFRSNPRYAAKLAESYRLPS
EVVEWLAGPRAKDVLNYIKKRRVGK

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=427, Percent_Identity=26.2295081967213, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI6677723, Length=222, Percent_Identity=31.5315315315315, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI31563534, Length=232, Percent_Identity=27.5862068965517, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI4506491, Length=231, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI31881687, Length=231, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI194306571, Length=217, Percent_Identity=31.3364055299539, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI194306567, Length=217, Percent_Identity=31.3364055299539, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17563226, Length=351, Percent_Identity=29.9145299145299, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17541988, Length=224, Percent_Identity=30.8035714285714, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17541368, Length=252, Percent_Identity=27.3809523809524, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554730, Length=216, Percent_Identity=32.4074074074074, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71986063, Length=231, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324791, Length=301, Percent_Identity=26.9102990033223, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323724, Length=255, Percent_Identity=25.0980392156863, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324478, Length=233, Percent_Identity=29.1845493562232, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322528, Length=234, Percent_Identity=31.1965811965812, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324039, Length=217, Percent_Identity=34.1013824884793, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17737765, Length=341, Percent_Identity=29.3255131964809, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI62472391, Length=363, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28574716, Length=199, Percent_Identity=30.6532663316583, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17647857, Length=222, Percent_Identity=27.9279279279279, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859927, Length=216, Percent_Identity=33.7962962962963, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19921076, Length=224, Percent_Identity=31.25, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: NA

Molecular weight: Translated: 48470; Mature: 48338

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQERVPWIIKYRPKTLDEVVNQDEAKSKLLEWLSSWEKGKPSKKAALLHGPPGCGKTSL
CCCCCCCEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHH
VEALARSKGYQLLEMNASDARRKEDIERIVKLASRSGALTGSRKIILLDEVDGMDVRADA
HHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCC
GGVEALVEVIKVSANPIIMTANNPYSQMLRPLRELSEMIAFKRLTPRDVVTVLKRICSAE
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KLVCEDQALDEIAKRSEGDLRSAINDLEAMAGASERITLGLVKSFSTYRDRTYAPYEALQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
KLFNSRYIFQAREAPTSTDLTPDEFMVWINEHIPTYYEDLEEIVRAYDALSKADVYMGRI
HHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHE
IKTNNWDLLTYALDMMGPGVAFARKTYRYRWKPFKQPERIKLMSETKKSREIREALAEQL
EECCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASHILASKAVVKSDVIPFLRVIFRSNPRYAAKLAESYRLPSEVVEWLAGPRAKDVLNYIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHH
KRRVGK
HHCCCC
>Mature Secondary Structure 
SQERVPWIIKYRPKTLDEVVNQDEAKSKLLEWLSSWEKGKPSKKAALLHGPPGCGKTSL
CCCCCCEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHH
VEALARSKGYQLLEMNASDARRKEDIERIVKLASRSGALTGSRKIILLDEVDGMDVRADA
HHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCC
GGVEALVEVIKVSANPIIMTANNPYSQMLRPLRELSEMIAFKRLTPRDVVTVLKRICSAE
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KLVCEDQALDEIAKRSEGDLRSAINDLEAMAGASERITLGLVKSFSTYRDRTYAPYEALQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
KLFNSRYIFQAREAPTSTDLTPDEFMVWINEHIPTYYEDLEEIVRAYDALSKADVYMGRI
HHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHE
IKTNNWDLLTYALDMMGPGVAFARKTYRYRWKPFKQPERIKLMSETKKSREIREALAEQL
EECCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASHILASKAVVKSDVIPFLRVIFRSNPRYAAKLAESYRLPSEVVEWLAGPRAKDVLNYIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHH
KRRVGK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA