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The map label for this gene is rfc [H]

Identifier: 218884000

GI number: 218884000

Start: 659483

End: 660463

Strand: Direct

Name: rfc [H]

Synonym: DKAM_0689

Alternate gene names: 218884000

Gene position: 659483-660463 (Clockwise)

Preceding gene: 218883997

Following gene: 218884001

Centisome position: 48.31

GC content: 47.3

Gene sequence:

>981_bases
TTGAGTGAGGAAGTAGAATTATTGTGGGCTGAGAAATATCGTCCTAGAACACTCGACGAGGTAGTGAATCAGAAGGAAGT
AGTCGTCAGGCTGAAGAAGTTCGTTGAGGAGAAGAACATACCCCACATGCTCTTCGCTGGACCTCCTGGCACAGGTAAGA
CCACCATAGCTCATTGCCTAGCCCATGATCTCTACGGCGACGACTATAGGAAGTATATGCTGGAGTTAAACGCGTCGGAT
GAGAGGAAGATAGAGGTTATAAGAGGCAAGGTTAAGGAGTTCGCAAGGACTAGAGTCGTCGGTGATGTACCATTTAAAAT
AGTGCTTCTCGACGAAGCCGATAACATGACGGCTGATGCTCAGCAAGCCCTGAGGAGGCTCATGGAGCTGTATAGTGCTA
CAACAAGGTTTATTCTGACGGCAAACTACCCAAGCAAGATCATAGAGCCTATTCAAAGTAGAACAGCCATCTTCAGGTTT
TCGCCGCTAAGCAGGGAGGATGTTGTAGGGAGGTTGAAATATATATGCAACGCTGAGAAGATAGAATGCGCTGAGAAAGC
GCTTGAAACAATATATGAACTATCAGAGGGTGATATGAGGCGGGCCATAAACATCCTGCAGACAGCGGCCGCCTTAGGCG
AGGTAGTCGAGGAAGCCGTCTACAAGGTACTAGGGATGGCGCATCCCAGGGAAGTCAGAGAGATGATAAATACAGCACTA
GCAGGCAACTTCACGGAAGCCAGGAATAAGTTAAGAACCTTGATGATCGAATACGGGCTCAGCGGCCTCGACATTGTGAA
GCAGATACATAGAGAGATATTCAGTCAGGATGTTAAAATACCGGATGAGATAAGGGTGTTAATAGCTGATCTAGCTGGTG
AAATACAGTTCAGGCTTGTCGAGGGAGCCGATGACGAGATACAGTTGAACGCGTTTCTAGCCAGGCTAGCTTTAATAGGT
AAGAAATTCAAGACGGGGTAG

Upstream 100 bases:

>100_bases
TAAACCATTCAAAGCATCCACCATTTAGTTATAATACCCTGAAATCCCTTTAATATCCTAGCCGCATTATACCTAATAAT
GAAGCCTTTAGGTGTCTAGT

Downstream 100 bases:

>100_bases
CCCTTGAGCCAGGAGAGAGTACCGTGGATAATAAAATATAGGCCCAAGACCCTTGACGAGGTAGTAAACCAGGATGAAGC
CAAGTCAAAGCTACTGGAAT

Product: replication factor C small subunit

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSEEVELLWAEKYRPRTLDEVVNQKEVVVRLKKFVEEKNIPHMLFAGPPGTGKTTIAHCLAHDLYGDDYRKYMLELNASD
ERKIEVIRGKVKEFARTRVVGDVPFKIVLLDEADNMTADAQQALRRLMELYSATTRFILTANYPSKIIEPIQSRTAIFRF
SPLSREDVVGRLKYICNAEKIECAEKALETIYELSEGDMRRAINILQTAAALGEVVEEAVYKVLGMAHPREVREMINTAL
AGNFTEARNKLRTLMIEYGLSGLDIVKQIHREIFSQDVKIPDEIRVLIADLAGEIQFRLVEGADDEIQLNAFLARLALIG
KKFKTG

Sequences:

>Translated_326_residues
MSEEVELLWAEKYRPRTLDEVVNQKEVVVRLKKFVEEKNIPHMLFAGPPGTGKTTIAHCLAHDLYGDDYRKYMLELNASD
ERKIEVIRGKVKEFARTRVVGDVPFKIVLLDEADNMTADAQQALRRLMELYSATTRFILTANYPSKIIEPIQSRTAIFRF
SPLSREDVVGRLKYICNAEKIECAEKALETIYELSEGDMRRAINILQTAAALGEVVEEAVYKVLGMAHPREVREMINTAL
AGNFTEARNKLRTLMIEYGLSGLDIVKQIHREIFSQDVKIPDEIRVLIADLAGEIQFRLVEGADDEIQLNAFLARLALIG
KKFKTG
>Mature_325_residues
SEEVELLWAEKYRPRTLDEVVNQKEVVVRLKKFVEEKNIPHMLFAGPPGTGKTTIAHCLAHDLYGDDYRKYMLELNASDE
RKIEVIRGKVKEFARTRVVGDVPFKIVLLDEADNMTADAQQALRRLMELYSATTRFILTANYPSKIIEPIQSRTAIFRFS
PLSREDVVGRLKYICNAEKIECAEKALETIYELSEGDMRRAINILQTAAALGEVVEEAVYKVLGMAHPREVREMINTALA
GNFTEARNKLRTLMIEYGLSGLDIVKQIHREIFSQDVKIPDEIRVLIADLAGEIQFRLVEGADDEIQLNAFLARLALIGK
KFKTG

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily [H]

Homologues:

Organism=Homo sapiens, GI6677723, Length=310, Percent_Identity=41.9354838709677, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI194306571, Length=306, Percent_Identity=41.8300653594771, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI194306567, Length=306, Percent_Identity=41.8300653594771, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI31563534, Length=316, Percent_Identity=41.7721518987342, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI4506491, Length=317, Percent_Identity=40.3785488958991, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI31881687, Length=317, Percent_Identity=40.3785488958991, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI28882049, Length=313, Percent_Identity=37.0607028753994, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI194306569, Length=243, Percent_Identity=42.3868312757202, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI4506489, Length=355, Percent_Identity=28.4507042253521, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI108773789, Length=225, Percent_Identity=33.7777777777778, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1786676, Length=238, Percent_Identity=28.9915966386555, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71986063, Length=314, Percent_Identity=41.4012738853503, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17541988, Length=313, Percent_Identity=40.2555910543131, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17554730, Length=319, Percent_Identity=36.3636363636364, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17541990, Length=228, Percent_Identity=35.5263157894737, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324478, Length=323, Percent_Identity=43.343653250774, Blast_Score=254, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6324039, Length=314, Percent_Identity=38.2165605095541, Blast_Score=235, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6322528, Length=327, Percent_Identity=40.3669724770642, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6319562, Length=244, Percent_Identity=30.7377049180328, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324791, Length=235, Percent_Identity=26.8085106382979, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17647857, Length=321, Percent_Identity=42.3676012461059, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI19921076, Length=314, Percent_Identity=38.2165605095541, Blast_Score=220, Evalue=9e-58,
Organism=Drosophila melanogaster, GI18859927, Length=314, Percent_Identity=39.4904458598726, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI19921136, Length=346, Percent_Identity=28.6127167630058, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR000641
- InterPro:   IPR008921
- InterPro:   IPR013748 [H]

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 37059; Mature: 36928

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00039 DEAD_ATP_HELICASE ; PS01019 ARF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEVELLWAEKYRPRTLDEVVNQKEVVVRLKKFVEEKNIPHMLFAGPPGTGKTTIAHCL
CCCCHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
AHDLYGDDYRKYMLELNASDERKIEVIRGKVKEFARTRVVGDVPFKIVLLDEADNMTADA
HHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
QQALRRLMELYSATTRFILTANYPSKIIEPIQSRTAIFRFSPLSREDVVGRLKYICNAEK
HHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCHH
IECAEKALETIYELSEGDMRRAINILQTAAALGEVVEEAVYKVLGMAHPREVREMINTAL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AGNFTEARNKLRTLMIEYGLSGLDIVKQIHREIFSQDVKIPDEIRVLIADLAGEIQFRLV
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEE
EGADDEIQLNAFLARLALIGKKFKTG
CCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEEVELLWAEKYRPRTLDEVVNQKEVVVRLKKFVEEKNIPHMLFAGPPGTGKTTIAHCL
CCCHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
AHDLYGDDYRKYMLELNASDERKIEVIRGKVKEFARTRVVGDVPFKIVLLDEADNMTADA
HHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
QQALRRLMELYSATTRFILTANYPSKIIEPIQSRTAIFRFSPLSREDVVGRLKYICNAEK
HHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCHH
IECAEKALETIYELSEGDMRRAINILQTAAALGEVVEEAVYKVLGMAHPREVREMINTAL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AGNFTEARNKLRTLMIEYGLSGLDIVKQIHREIFSQDVKIPDEIRVLIADLAGEIQFRLV
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEE
EGADDEIQLNAFLARLALIGKKFKTG
CCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA