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The map label for this gene is yfjR [H]

Identifier: 218883986

GI number: 218883986

Start: 646652

End: 647515

Strand: Direct

Name: yfjR [H]

Synonym: DKAM_0675

Alternate gene names: 218883986

Gene position: 646652-647515 (Clockwise)

Preceding gene: 218883985

Following gene: 218883987

Centisome position: 47.37

GC content: 46.18

Gene sequence:

>864_bases
ATGGTTTACAGGATCCTTGTTGCTGGAGCTGGTTTAATGGGTTCTTCACTGGTTAGATGCCTGGTATCCCGTGGTGTCGA
GGTATTTGTCTACAATAGAAACGTTGAGAAGGCTAGATCCCTTTGCTCCACCGTTAAATGCAGGGTCATTGAGGATCTAG
ATTTAATGGATAACGTTGATGCATCGGTGATCTTCTTATTTGACGATCAAGCCGTCATGGACTTCGTTTACACGCTCATA
GAGAAGAACTTGTTAAGCAGGACGGGTATTCTAATGAATTCTTCAACCATAAGCCCGGAGACTAGTTTAATGCTGTATAG
GATGGTTAGAGATCATGGGGGCATATACATTGAAGCACCTGTCTACGGGAGTACCGATGAAGCCTCAGCATGCAACCTTG
TCACAATGCTCGCGGGGGATTCAGGGATAGAGGGTAAGGCTAGGGAGATAGCGCTACTCTACTCTAGTAAAATATACTGG
GTTGGCGGGATCCCTAAGGCCATGGCTTTAAAACTGGCGTTGAATAATATAGGTCTCATAATGCCGGCTGTGCTAGCGGA
ATCCCTAGCACTCTTAGACTCGTATGACGTTGACCAGGAATTATTTAGTGAGATAGCCGGGAATCTATGGTTTGGAAACA
TGGTTAGGAGATATCTTGCTAGGATTAACATACCCGTTTCGACACCTAGGTTCACAATCAGCGGGGCCGCAAAGGATTAC
AGGGTTATCTCTTACACCCTTATGTTGAAAGGATATACGCCGCTACTCTCCCAGGCAGCCTCGAACTTTTACTCTCTAGC
AGCCAGGGACGTGCCTGGTGAAGACTATCCAAGGGCTGCATTGTACTATAGAAGGAAAACCTAG

Upstream 100 bases:

>100_bases
GCGTATCTCACAACTAACGGATCACAGCAGTGGAGGACGTTAACCTGAACCATTATAAACTGTTTAACTACTACTTCATA
GATATGGGTGGGATCTATTT

Downstream 100 bases:

>100_bases
AGCCAGAGACACGGGGTATTAAGTGTGGAAATAAGGCTCCTAGTTCCCGTGGATGTTATAATAGACTCCCGAGAGGATAG
TAAGCACCCTGAGTTCAGGG

Product: 6-phosphogluconate dehydrogenase, NAD-binding

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MVYRILVAGAGLMGSSLVRCLVSRGVEVFVYNRNVEKARSLCSTVKCRVIEDLDLMDNVDASVIFLFDDQAVMDFVYTLI
EKNLLSRTGILMNSSTISPETSLMLYRMVRDHGGIYIEAPVYGSTDEASACNLVTMLAGDSGIEGKAREIALLYSSKIYW
VGGIPKAMALKLALNNIGLIMPAVLAESLALLDSYDVDQELFSEIAGNLWFGNMVRRYLARINIPVSTPRFTISGAAKDY
RVISYTLMLKGYTPLLSQAASNFYSLAARDVPGEDYPRAALYYRRKT

Sequences:

>Translated_287_residues
MVYRILVAGAGLMGSSLVRCLVSRGVEVFVYNRNVEKARSLCSTVKCRVIEDLDLMDNVDASVIFLFDDQAVMDFVYTLI
EKNLLSRTGILMNSSTISPETSLMLYRMVRDHGGIYIEAPVYGSTDEASACNLVTMLAGDSGIEGKAREIALLYSSKIYW
VGGIPKAMALKLALNNIGLIMPAVLAESLALLDSYDVDQELFSEIAGNLWFGNMVRRYLARINIPVSTPRFTISGAAKDY
RVISYTLMLKGYTPLLSQAASNFYSLAARDVPGEDYPRAALYYRRKT
>Mature_287_residues
MVYRILVAGAGLMGSSLVRCLVSRGVEVFVYNRNVEKARSLCSTVKCRVIEDLDLMDNVDASVIFLFDDQAVMDFVYTLI
EKNLLSRTGILMNSSTISPETSLMLYRMVRDHGGIYIEAPVYGSTDEASACNLVTMLAGDSGIEGKAREIALLYSSKIYW
VGGIPKAMALKLALNNIGLIMPAVLAESLALLDSYDVDQELFSEIAGNLWFGNMVRRYLARINIPVSTPRFTISGAAKDY
RVISYTLMLKGYTPLLSQAASNFYSLAARDVPGEDYPRAALYYRRKT

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Drosophila melanogaster, GI28574115, Length=193, Percent_Identity=27.4611398963731, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31746; Mature: 31746

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYRILVAGAGLMGSSLVRCLVSRGVEVFVYNRNVEKARSLCSTVKCRVIEDLDLMDNVD
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHCCCC
ASVIFLFDDQAVMDFVYTLIEKNLLSRTGILMNSSTISPETSLMLYRMVRDHGGIYIEAP
CEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEEC
VYGSTDEASACNLVTMLAGDSGIEGKAREIALLYSSKIYWVGGIPKAMALKLALNNIGLI
CCCCCCCCCCCCEEEEEECCCCCCCCHHEEEEEEECEEEEECCCCHHHHHHHHHHCCHHH
MPAVLAESLALLDSYDVDQELFSEIAGNLWFGNMVRRYLARINIPVSTPRFTISGAAKDY
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCE
RVISYTLMLKGYTPLLSQAASNFYSLAARDVPGEDYPRAALYYRRKT
EEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEECCC
>Mature Secondary Structure
MVYRILVAGAGLMGSSLVRCLVSRGVEVFVYNRNVEKARSLCSTVKCRVIEDLDLMDNVD
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHCCCC
ASVIFLFDDQAVMDFVYTLIEKNLLSRTGILMNSSTISPETSLMLYRMVRDHGGIYIEAP
CEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEEC
VYGSTDEASACNLVTMLAGDSGIEGKAREIALLYSSKIYWVGGIPKAMALKLALNNIGLI
CCCCCCCCCCCCEEEEEECCCCCCCCHHEEEEEEECEEEEECCCCHHHHHHHHHHCCHHH
MPAVLAESLALLDSYDVDQELFSEIAGNLWFGNMVRRYLARINIPVSTPRFTISGAAKDY
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCE
RVISYTLMLKGYTPLLSQAASNFYSLAARDVPGEDYPRAALYYRRKT
EEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]