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The map label for this gene is purB [H]

Identifier: 218883841

GI number: 218883841

Start: 497739

End: 499082

Strand: Direct

Name: purB [H]

Synonym: DKAM_0530

Alternate gene names: 218883841

Gene position: 497739-499082 (Clockwise)

Preceding gene: 218883840

Following gene: 218883842

Centisome position: 36.46

GC content: 46.88

Gene sequence:

>1344_bases
ATGTCTGGAAGCATTTGCCCACTTGAATGGAGATATGGTTCAACCGAGATGAGAAGGGTGTTTTCCAGGAGAAATATAGT
GGATACATACATAGCCGTGGAAGCAGCTATATTAAAGGGCCTCGAGGAGGCTGGATTGGCACCTAGTGGTTGCTGGAGTA
AGGTAGAATCATTGAAAGGGACACTAGATCCCGCCGAGGTTGATAGGCTTGAATCCAGGATAGGACACGATATAGCCGCA
ATCGCCGAAATAATATCGAACAGGATTGATGAGCCATGTAGTAGATTCGTGCACCTAGGGGCCACCAGCTACGATATAGT
TGACACAACGTGGGCTCTAATACTTGGCGAGGCATTAAAGCTCACTAGGGCCAGGCTTATAGAGGTGATTAAACTACTGG
CCAGCCTGGTTGAGAAATACCGTGATACCATTGTGGTGGGTAGAACCCATGCCAGGCATGCATTACCGATTACATTCGGG
TTTAAGATGGCTAACTACGCCTATGAGTTAACGAGATCGCTTCAAAGGCTCAGGGAACTCAATGAACGCGTCGTTAGGAG
CAAGATATCGGGTGCTGTGGGAACCATGGCTGGCTGGCTGAAGCAGGGATTAATAGTTGAGAATACTGCTTCAAGCCATT
TAGGCCTCAAGCCCCACTTGATAACGACTCAGGTTGCACCCAGGGATGGCTTCGCCGAGGTGATTGCTGACCTAGCTATA
CTGGCTTCACAGCTAGAGAGATTGTCACTGGAGGTAAGGGAGTTGATGAGGGATGAGATCTCCGAGGTGTACATCGATAC
CGGTGAGGTGGGTAGCAGTGCAATGCCCCATAAGAGAAACCCATCTGTACCTGAAAGAGTATGCGGTTTATCAAGGGTCA
TAAGGGGGTTGCTCGTTTCATCCATGGAGAACATAGCTTTAATGCATGAGAGGGACCTCACTAATAGTAGCTCCGAGAGA
GTGATCATACCACACGTCTTCCTCATAATTGATCAAATGCTGCTCGACACAATAAGTATTCTTAGAGGGCTGCGTGTAGA
TGAGGTAAGCACTAGTAGAAACCTTGAGTTAACGCAAGGCAGGATAATGAGTGAATGCATTATGGTGAAACTGGTGCTTA
GAAAAGGCTACTCGAGGATCCAGGCACATGAGTTACTTAGAAAGCTGAGCAGGGAAAGTATACGGGAGAACAAGCATTTA
AGAGAGATACTGATGGAGAACGGGTTATATGATGATGAGATAGGCGAGTGCTTTGACTACTCGAAATACCTTGGCAACTA
CTCTGAGCTCATAGATAGAGTCTTGAAATATGTTAGAGAGCAGATTGAAGTAGGGAGCATGTGA

Upstream 100 bases:

>100_bases
TTTCGGGAAACCAATCAGTATGGGGAGGAGGATCGCTATCGAGATACATGAATGCATTGAATCAGGTTGCTTACAGGAGA
TAGTCACGTGAGGTGTTCGC

Downstream 100 bases:

>100_bases
TTATGAACATCGATGAATTACTCAGGGGCTATGATACAGGTAACCTAGCGATCGGCACGCTTGCAAGTCACTCGGCACTA
CAGATAATGCATGGAGCTAA

Product: Adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSGSICPLEWRYGSTEMRRVFSRRNIVDTYIAVEAAILKGLEEAGLAPSGCWSKVESLKGTLDPAEVDRLESRIGHDIAA
IAEIISNRIDEPCSRFVHLGATSYDIVDTTWALILGEALKLTRARLIEVIKLLASLVEKYRDTIVVGRTHARHALPITFG
FKMANYAYELTRSLQRLRELNERVVRSKISGAVGTMAGWLKQGLIVENTASSHLGLKPHLITTQVAPRDGFAEVIADLAI
LASQLERLSLEVRELMRDEISEVYIDTGEVGSSAMPHKRNPSVPERVCGLSRVIRGLLVSSMENIALMHERDLTNSSSER
VIIPHVFLIIDQMLLDTISILRGLRVDEVSTSRNLELTQGRIMSECIMVKLVLRKGYSRIQAHELLRKLSRESIRENKHL
REILMENGLYDDEIGECFDYSKYLGNYSELIDRVLKYVREQIEVGSM

Sequences:

>Translated_447_residues
MSGSICPLEWRYGSTEMRRVFSRRNIVDTYIAVEAAILKGLEEAGLAPSGCWSKVESLKGTLDPAEVDRLESRIGHDIAA
IAEIISNRIDEPCSRFVHLGATSYDIVDTTWALILGEALKLTRARLIEVIKLLASLVEKYRDTIVVGRTHARHALPITFG
FKMANYAYELTRSLQRLRELNERVVRSKISGAVGTMAGWLKQGLIVENTASSHLGLKPHLITTQVAPRDGFAEVIADLAI
LASQLERLSLEVRELMRDEISEVYIDTGEVGSSAMPHKRNPSVPERVCGLSRVIRGLLVSSMENIALMHERDLTNSSSER
VIIPHVFLIIDQMLLDTISILRGLRVDEVSTSRNLELTQGRIMSECIMVKLVLRKGYSRIQAHELLRKLSRESIRENKHL
REILMENGLYDDEIGECFDYSKYLGNYSELIDRVLKYVREQIEVGSM
>Mature_446_residues
SGSICPLEWRYGSTEMRRVFSRRNIVDTYIAVEAAILKGLEEAGLAPSGCWSKVESLKGTLDPAEVDRLESRIGHDIAAI
AEIISNRIDEPCSRFVHLGATSYDIVDTTWALILGEALKLTRARLIEVIKLLASLVEKYRDTIVVGRTHARHALPITFGF
KMANYAYELTRSLQRLRELNERVVRSKISGAVGTMAGWLKQGLIVENTASSHLGLKPHLITTQVAPRDGFAEVIADLAIL
ASQLERLSLEVRELMRDEISEVYIDTGEVGSSAMPHKRNPSVPERVCGLSRVIRGLLVSSMENIALMHERDLTNSSSERV
IIPHVFLIIDQMLLDTISILRGLRVDEVSTSRNLELTQGRIMSECIMVKLVLRKGYSRIQAHELLRKLSRESIRENKHLR
EILMENGLYDDEIGECFDYSKYLGNYSELIDRVLKYVREQIEVGSM

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=451, Percent_Identity=26.1640798226164, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI183227688, Length=387, Percent_Identity=26.6149870801034, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1787376, Length=419, Percent_Identity=28.4009546539379, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17508577, Length=337, Percent_Identity=27.5964391691395, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=304, Percent_Identity=26.9736842105263, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323391, Length=405, Percent_Identity=26.6666666666667, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647570, Length=472, Percent_Identity=27.7542372881356, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24662684, Length=184, Percent_Identity=32.0652173913043, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50374; Mature: 50243

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSICPLEWRYGSTEMRRVFSRRNIVDTYIAVEAAILKGLEEAGLAPSGCWSKVESLKG
CCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
TLDPAEVDRLESRIGHDIAAIAEIISNRIDEPCSRFVHLGATSYDIVDTTWALILGEALK
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LTRARLIEVIKLLASLVEKYRDTIVVGRTHARHALPITFGFKMANYAYELTRSLQRLREL
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
NERVVRSKISGAVGTMAGWLKQGLIVENTASSHLGLKPHLITTQVAPRDGFAEVIADLAI
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHH
LASQLERLSLEVRELMRDEISEVYIDTGEVGSSAMPHKRNPSVPERVCGLSRVIRGLLVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SMENIALMHERDLTNSSSERVIIPHVFLIIDQMLLDTISILRGLRVDEVSTSRNLELTQG
HHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RIMSECIMVKLVLRKGYSRIQAHELLRKLSRESIRENKHLREILMENGLYDDEIGECFDY
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SKYLGNYSELIDRVLKYVREQIEVGSM
HHHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGSICPLEWRYGSTEMRRVFSRRNIVDTYIAVEAAILKGLEEAGLAPSGCWSKVESLKG
CCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
TLDPAEVDRLESRIGHDIAAIAEIISNRIDEPCSRFVHLGATSYDIVDTTWALILGEALK
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LTRARLIEVIKLLASLVEKYRDTIVVGRTHARHALPITFGFKMANYAYELTRSLQRLREL
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
NERVVRSKISGAVGTMAGWLKQGLIVENTASSHLGLKPHLITTQVAPRDGFAEVIADLAI
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHH
LASQLERLSLEVRELMRDEISEVYIDTGEVGSSAMPHKRNPSVPERVCGLSRVIRGLLVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SMENIALMHERDLTNSSSERVIIPHVFLIIDQMLLDTISILRGLRVDEVSTSRNLELTQG
HHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RIMSECIMVKLVLRKGYSRIQAHELLRKLSRESIRENKHLREILMENGLYDDEIGECFDY
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SKYLGNYSELIDRVLKYVREQIEVGSM
HHHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]