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The map label for this gene is pdxT [H]

Identifier: 218883792

GI number: 218883792

Start: 448707

End: 449306

Strand: Reverse

Name: pdxT [H]

Synonym: DKAM_0481

Alternate gene names: 218883792

Gene position: 449306-448707 (Counterclockwise)

Preceding gene: 218883793

Following gene: 218883791

Centisome position: 32.91

GC content: 47.67

Gene sequence:

>600_bases
ATGATTAAAATAGGCGTCTTAGCTCTGCAGGGAGACTACCTTGAGCATTACCAGGTGCTTAAATCGCTTGGAGGAGTTGA
ACCAGTGATCGTTAAGCATTCAAAGGAGCTGTCAGACATTGATGCATTGATAATACCAGGCGGTGAATCCACGACAATAG
GCTCATTAATAGAGAACAGGATGCTCAGCAAGCCCTTAGTGGAGTTTATGGAGACCGGTAAACCAGTTCTAGGCACCTGT
GCCGGCGCAATACTCCTGGCTAAAGAGGTAGTCGACAGGGTTGTTGGGGAGACAAAACAGTATACTCTCAGAGCAATGAA
TATCAGGATAATGAGGAACGCGTATGGGAGGCAAAGGGAATCATTCATTGCGACGGTCGATATTGAGGATATAGGGGAGG
TGCGGGCAGCGTTTATAAGAGCTCCTATAATACTCGAGGCGCGTGAGCCTGCGAGGATAACTGGTTACCTCAACGATTCC
TCGCTGGGTAGGCATGGTGTTGCTGCACGTGAGAAGAACCTGTACGCAGTGACATTCCATCCCGAGATAACGGGTGATAT
AAGGCTCTATAACTATATTATCCAGCAAGCCAAGAGATAG

Upstream 100 bases:

>100_bases
TTGAGGCGCAGAAAATGGTGTCTGAGAAAGCTTCAATGCTCGGGATAGATATCAGGAGGCTTAAACCCGAGGAGTTGATG
CAGGAGCGAGGTGTGTAGTC

Downstream 100 bases:

>100_bases
TGTGAGGAATGCATCTTGGGCCAGGATACATGGGTTTTTTCACGGTGTCTGAAATGCGGGTGGAGTGAGAAAGGGTTACA
TCTTAAATGCCCTGTATGTG

Product: glutamine amidotransferase subunit pdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIKIGVLALQGDYLEHYQVLKSLGGVEPVIVKHSKELSDIDALIIPGGESTTIGSLIENRMLSKPLVEFMETGKPVLGTC
AGAILLAKEVVDRVVGETKQYTLRAMNIRIMRNAYGRQRESFIATVDIEDIGEVRAAFIRAPIILEAREPARITGYLNDS
SLGRHGVAAREKNLYAVTFHPEITGDIRLYNYIIQQAKR

Sequences:

>Translated_199_residues
MIKIGVLALQGDYLEHYQVLKSLGGVEPVIVKHSKELSDIDALIIPGGESTTIGSLIENRMLSKPLVEFMETGKPVLGTC
AGAILLAKEVVDRVVGETKQYTLRAMNIRIMRNAYGRQRESFIATVDIEDIGEVRAAFIRAPIILEAREPARITGYLNDS
SLGRHGVAAREKNLYAVTFHPEITGDIRLYNYIIQQAKR
>Mature_199_residues
MIKIGVLALQGDYLEHYQVLKSLGGVEPVIVKHSKELSDIDALIIPGGESTTIGSLIENRMLSKPLVEFMETGKPVLGTC
AGAILLAKEVVDRVVGETKQYTLRAMNIRIMRNAYGRQRESFIATVDIEDIGEVRAAFIRAPIILEAREPARITGYLNDS
SLGRHGVAAREKNLYAVTFHPEITGDIRLYNYIIQQAKR

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS [H]

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=211, Percent_Identity=34.5971563981043, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323995, Length=213, Percent_Identity=32.3943661971831, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321048, Length=214, Percent_Identity=32.7102803738318, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002161
- InterPro:   IPR021196 [H]

Pfam domain/function: PF01174 SNO [H]

EC number: NA

Molecular weight: Translated: 22064; Mature: 22064

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS01236 PDXT_SNO_1 ; PS51130 PDXT_SNO_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIGVLALQGDYLEHYQVLKSLGGVEPVIVKHSKELSDIDALIIPGGESTTIGSLIENR
CEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHH
MLSKPLVEFMETGKPVLGTCAGAILLAKEVVDRVVGETKQYTLRAMNIRIMRNAYGRQRE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHHCCCCCC
SFIATVDIEDIGEVRAAFIRAPIILEAREPARITGYLNDSSLGRHGVAAREKNLYAVTFH
CEEEEECHHHHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEC
PEITGDIRLYNYIIQQAKR
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIKIGVLALQGDYLEHYQVLKSLGGVEPVIVKHSKELSDIDALIIPGGESTTIGSLIENR
CEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHH
MLSKPLVEFMETGKPVLGTCAGAILLAKEVVDRVVGETKQYTLRAMNIRIMRNAYGRQRE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHHCCCCCC
SFIATVDIEDIGEVRAAFIRAPIILEAREPARITGYLNDSSLGRHGVAAREKNLYAVTFH
CEEEEECHHHHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEC
PEITGDIRLYNYIIQQAKR
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA