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The map label for this gene is pyrE [H]

Identifier: 218883787

GI number: 218883787

Start: 443925

End: 444548

Strand: Reverse

Name: pyrE [H]

Synonym: DKAM_0476

Alternate gene names: 218883787

Gene position: 444548-443925 (Counterclockwise)

Preceding gene: 218883788

Following gene: 218883786

Centisome position: 32.56

GC content: 49.36

Gene sequence:

>624_bases
GTGTCATGGATAAGCATTGAGCTCTATAAGAGGGGCATGGTGAAGATAGGGAGGTTCAAGCTATCCTCAGGCATAGAGAG
CCCATTCTACATCGATATGCGGAGACTATACATGTACCCGGATCTCGCTAGGATGATCATCCTGGAGCTAACACGTAGAG
TAAAACTCGACGATATCGATGTAGTAGTGGGTGTCGCGACAGCAGGAATACCTCTAGCTACATATGTCTCATGCCTCACA
AATAAGCCGATGGCATATGTCAGAGTGGAAAAGAAGAACCATGGAACCAGCTCGCTGATCGAGGGAGATGTAACTGGCTT
GAGAACCCTGATACTTGACGATGTATCTACAACGGGCTCCTCGATACTGAAGGCTGTCGAAGCAATATTGGAAGCAGGCG
GGATACCGGTGAGAGTAGCTGTCCTCGTTGACAGGGAGCAGGGAGCTCGTGAGGTACTTGCTTCCAGGGGTATCGAGCTA
TTCCGGTTAATAACAGCGAGAGAGGTCTTCAGGGATCTCTACGCTCATGGATTAATCAACGAGGCAACATACCGTGAGCT
ACTTGAGTACCTTGACAAGTACTCAGCAGGAAGAACGGTGAACCAGGGTGTACTACCCGGCTAA

Upstream 100 bases:

>100_bases
GTGCCAATTACGAGATAGTAGGTAGGCTGATAACCGGTTCAAAGAATCCTGGTGAAGCAGCCAGGGAGGTTAGGGAGATA
CAGTTGAAGAGGTGGTCGAA

Downstream 100 bases:

>100_bases
GATAACTGGCTCCGAGAAGCTTAGCAAGACACTGTATCTAAAGAGGATCAAGATACTCAGCGATGGAATCAAGGAACCCT
TACCATTTCAATTCCTGCTG

Product: Orotate phosphoribosyltransferase, PyrE

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSWISIELYKRGMVKIGRFKLSSGIESPFYIDMRRLYMYPDLARMIILELTRRVKLDDIDVVVGVATAGIPLATYVSCLT
NKPMAYVRVEKKNHGTSSLIEGDVTGLRTLILDDVSTTGSSILKAVEAILEAGGIPVRVAVLVDREQGAREVLASRGIEL
FRLITAREVFRDLYAHGLINEATYRELLEYLDKYSAGRTVNQGVLPG

Sequences:

>Translated_207_residues
MSWISIELYKRGMVKIGRFKLSSGIESPFYIDMRRLYMYPDLARMIILELTRRVKLDDIDVVVGVATAGIPLATYVSCLT
NKPMAYVRVEKKNHGTSSLIEGDVTGLRTLILDDVSTTGSSILKAVEAILEAGGIPVRVAVLVDREQGAREVLASRGIEL
FRLITAREVFRDLYAHGLINEATYRELLEYLDKYSAGRTVNQGVLPG
>Mature_206_residues
SWISIELYKRGMVKIGRFKLSSGIESPFYIDMRRLYMYPDLARMIILELTRRVKLDDIDVVVGVATAGIPLATYVSCLTN
KPMAYVRVEKKNHGTSSLIEGDVTGLRTLILDDVSTTGSSILKAVEAILEAGGIPVRVAVLVDREQGAREVLASRGIELF
RLITAREVFRDLYAHGLINEATYRELLEYLDKYSAGRTVNQGVLPG

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=181, Percent_Identity=38.6740331491713, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1790073, Length=201, Percent_Identity=26.865671641791, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554964, Length=190, Percent_Identity=32.1052631578947, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17508631, Length=189, Percent_Identity=33.8624338624339, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323530, Length=205, Percent_Identity=28.2926829268293, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17933654, Length=194, Percent_Identity=34.020618556701, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23040; Mature: 22909

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWISIELYKRGMVKIGRFKLSSGIESPFYIDMRRLYMYPDLARMIILELTRRVKLDDID
CCEEEEHHHHCCHHHHHHEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHH
VVVGVATAGIPLATYVSCLTNKPMAYVRVEKKNHGTSSLIEGDVTGLRTLILDDVSTTGS
EEEEHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCHHHHHHHHHHHCCCCCHH
SILKAVEAILEAGGIPVRVAVLVDREQGAREVLASRGIELFRLITAREVFRDLYAHGLIN
HHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
EATYRELLEYLDKYSAGRTVNQGVLPG
HHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SWISIELYKRGMVKIGRFKLSSGIESPFYIDMRRLYMYPDLARMIILELTRRVKLDDID
CEEEEHHHHCCHHHHHHEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHH
VVVGVATAGIPLATYVSCLTNKPMAYVRVEKKNHGTSSLIEGDVTGLRTLILDDVSTTGS
EEEEHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCHHHHHHHHHHHCCCCCHH
SILKAVEAILEAGGIPVRVAVLVDREQGAREVLASRGIELFRLITAREVFRDLYAHGLIN
HHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
EATYRELLEYLDKYSAGRTVNQGVLPG
HHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12142413 [H]