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The map label for this gene is nuoN [H]

Identifier: 218883720

GI number: 218883720

Start: 384568

End: 386160

Strand: Reverse

Name: nuoN [H]

Synonym: DKAM_0409

Alternate gene names: 218883720

Gene position: 386160-384568 (Counterclockwise)

Preceding gene: 218883721

Following gene: 218883719

Centisome position: 28.29

GC content: 46.01

Gene sequence:

>1593_bases
ATGCTGGATCAACTAGCATTTAAACTAGCTGGGTTAACAGTCCCATTGCTTGCACTCATAGCGTTCCTCATACCTTTACT
CGAGAAGCTCACGGGTAGTAGGGATGCCCCGAGGATCCTAACCTTATTGGGGTCGCTGATAGCTGTTTTTTCGTCTCTAA
TAATCCTCATTAGTGTATATACTAGTGGAGAGGTGATCCTATACCCATTTGGTGGATGGCCCCCACACTTCGGTATAGTC
TACGAGATAGATGAGTTAAACGCGTTGATTGGTGCATACATCGCATTAGCGATGCTTGCCGTGGTTGCGTACTCTACATG
GTATGGCCGCCACCTTGATGAACCTATATGGTACTATGTACTCCTCACAGGATTAGAGATAGGTATGCTTGGATGCGTTT
ACACAGGCGATGCATTCAACCTCTTCGTAATGCTCGAGGTTCTCAGCGTATCTGCCTACGGATTAGTGGCCTACCATAAG
AATAGGGCTGAGGCTGTTGAGGCAGCAGCGAAATACGCGTTAATCGGTGCTGTAGCCACGAATCTTTACTTTGCTGCATT
AGTAATAATTTACGTGACCCATGGCTCGTTGAACATGGGATTATTATCACTGATTGCACGACAATATAGTGGTGCAGGGG
AGGCAGTGAAATACGCTGGCTTAATAGCTGTAGCCTTCTCGCTATGGGTCTTCACTTATAAGTCAGCATTATTCCCCTCA
CACTTCTGGCTACCTGACGCACATCCGGAAGCCCCTACACCTGTTTCGGCAACTTTATCCGGTGTTCTTGTAAACGTGGG
AGTATATGCTGTAATGAGGTTCCTCTACACTATATTCGGTTCTGATAGTTACCTTGCTGAGTTCAGGGCGATCGTACTGG
CTACATTATTTATCCTGGGTTCTCTAAGCGGATTAATCGGGGCATTAATGATGATGATGCAGAAGGATGTGAAGAGGCTG
CTCGCATATAGTACCATTAGTCATATTGGGATAATATATATAGGATTATCAATAGGCTTCCTCTCAGGTGTTGGGGAAGC
TGTAAAGATAGCCATGGCAGGCGCCATAGCCCACATGATCTCACATGGTATAGCGAAGACGATGCTCTTCATGGCAAGCG
GGGTATTCATCGATGCCGCTGGCTCCAGGGACCTGGATGAATTAAGAGGAATTGGTAGAAATTATCCGATCGCCAGCTTT
GCATGGTTAACCGGGTTCCTTAGTCTCGCAGGGCTGCTGCCGTTCTTAGGCTTCTACTCTAAGCTACTAATATACTATGG
CTACACCTATTCAGGCTTCATACTTGGAGGCGTAATGATAATAGTGATATCAGCTCTCTCCCTACCGGGGTGCTTCAAGG
CCATATACTCGGTTATATTCAGCATTGGCAGGGAAAGGGAGAAAACCAATGCGGGAACAGCTGAGGTAATAATTTTTATC
ATGGCGATTACACTGGTGATCCTGGGCCTCCTGTTCCCATTCATGGAAAACATATTCATGAAGGCTTCGGAGGCTATTAC
AACCCGTGTAGGGGTATATGATTATTCTTTGAAAGCGTATCATGAAATACTAAGGATCTTAGGAGGTGGGTAG

Upstream 100 bases:

>100_bases
GATTGATGCTAAAACCGTGGAACCCAGCGGGGCCAGGAATGAGATATCTTTTGTTTTCGAAAAGTATTCCCGTAAAATAT
TCGAGTGAGAGGTGTAGATC

Downstream 100 bases:

>100_bases
ACTTGATAATCGAGGTAGTAATGGATCCATCCATTACAGCATTAATTATTCTAGCTGGAAGCGGGCTAACACTACTTGTA
GCAGCTACATTATACTATCT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLDQLAFKLAGLTVPLLALIAFLIPLLEKLTGSRDAPRILTLLGSLIAVFSSLIILISVYTSGEVILYPFGGWPPHFGIV
YEIDELNALIGAYIALAMLAVVAYSTWYGRHLDEPIWYYVLLTGLEIGMLGCVYTGDAFNLFVMLEVLSVSAYGLVAYHK
NRAEAVEAAAKYALIGAVATNLYFAALVIIYVTHGSLNMGLLSLIARQYSGAGEAVKYAGLIAVAFSLWVFTYKSALFPS
HFWLPDAHPEAPTPVSATLSGVLVNVGVYAVMRFLYTIFGSDSYLAEFRAIVLATLFILGSLSGLIGALMMMMQKDVKRL
LAYSTISHIGIIYIGLSIGFLSGVGEAVKIAMAGAIAHMISHGIAKTMLFMASGVFIDAAGSRDLDELRGIGRNYPIASF
AWLTGFLSLAGLLPFLGFYSKLLIYYGYTYSGFILGGVMIIVISALSLPGCFKAIYSVIFSIGREREKTNAGTAEVIIFI
MAITLVILGLLFPFMENIFMKASEAITTRVGVYDYSLKAYHEILRILGGG

Sequences:

>Translated_530_residues
MLDQLAFKLAGLTVPLLALIAFLIPLLEKLTGSRDAPRILTLLGSLIAVFSSLIILISVYTSGEVILYPFGGWPPHFGIV
YEIDELNALIGAYIALAMLAVVAYSTWYGRHLDEPIWYYVLLTGLEIGMLGCVYTGDAFNLFVMLEVLSVSAYGLVAYHK
NRAEAVEAAAKYALIGAVATNLYFAALVIIYVTHGSLNMGLLSLIARQYSGAGEAVKYAGLIAVAFSLWVFTYKSALFPS
HFWLPDAHPEAPTPVSATLSGVLVNVGVYAVMRFLYTIFGSDSYLAEFRAIVLATLFILGSLSGLIGALMMMMQKDVKRL
LAYSTISHIGIIYIGLSIGFLSGVGEAVKIAMAGAIAHMISHGIAKTMLFMASGVFIDAAGSRDLDELRGIGRNYPIASF
AWLTGFLSLAGLLPFLGFYSKLLIYYGYTYSGFILGGVMIIVISALSLPGCFKAIYSVIFSIGREREKTNAGTAEVIIFI
MAITLVILGLLFPFMENIFMKASEAITTRVGVYDYSLKAYHEILRILGGG
>Mature_530_residues
MLDQLAFKLAGLTVPLLALIAFLIPLLEKLTGSRDAPRILTLLGSLIAVFSSLIILISVYTSGEVILYPFGGWPPHFGIV
YEIDELNALIGAYIALAMLAVVAYSTWYGRHLDEPIWYYVLLTGLEIGMLGCVYTGDAFNLFVMLEVLSVSAYGLVAYHK
NRAEAVEAAAKYALIGAVATNLYFAALVIIYVTHGSLNMGLLSLIARQYSGAGEAVKYAGLIAVAFSLWVFTYKSALFPS
HFWLPDAHPEAPTPVSATLSGVLVNVGVYAVMRFLYTIFGSDSYLAEFRAIVLATLFILGSLSGLIGALMMMMQKDVKRL
LAYSTISHIGIIYIGLSIGFLSGVGEAVKIAMAGAIAHMISHGIAKTMLFMASGVFIDAAGSRDLDELRGIGRNYPIASF
AWLTGFLSLAGLLPFLGFYSKLLIYYGYTYSGFILGGVMIIVISALSLPGCFKAIYSVIFSIGREREKTNAGTAEVIIFI
MAITLVILGLLFPFMENIFMKASEAITTRVGVYDYSLKAYHEILRILGGG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=328, Percent_Identity=28.3536585365854, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI251831116, Length=351, Percent_Identity=26.7806267806268, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1788831, Length=292, Percent_Identity=34.2465753424658, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788829, Length=423, Percent_Identity=29.3144208037825, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788827, Length=381, Percent_Identity=25.7217847769029, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI2367154, Length=438, Percent_Identity=26.4840182648402, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788613, Length=326, Percent_Identity=27.9141104294479, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI145693160, Length=344, Percent_Identity=29.9418604651163, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788614, Length=360, Percent_Identity=28.0555555555556, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 57321; Mature: 57321

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQLAFKLAGLTVPLLALIAFLIPLLEKLTGSRDAPRILTLLGSLIAVFSSLIILISVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
TSGEVILYPFGGWPPHFGIVYEIDELNALIGAYIALAMLAVVAYSTWYGRHLDEPIWYYV
CCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LLTGLEIGMLGCVYTGDAFNLFVMLEVLSVSAYGLVAYHKNRAEAVEAAAKYALIGAVAT
HHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHECCCHHHHHHHHHHHHHHHHHHH
NLYFAALVIIYVTHGSLNMGLLSLIARQYSGAGEAVKYAGLIAVAFSLWVFTYKSALFPS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
HFWLPDAHPEAPTPVSATLSGVLVNVGVYAVMRFLYTIFGSDSYLAEFRAIVLATLFILG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SLSGLIGALMMMMQKDVKRLLAYSTISHIGIIYIGLSIGFLSGVGEAVKIAMAGAIAHMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SHGIAKTMLFMASGVFIDAAGSRDLDELRGIGRNYPIASFAWLTGFLSLAGLLPFLGFYS
HHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KLLIYYGYTYSGFILGGVMIIVISALSLPGCFKAIYSVIFSIGREREKTNAGTAEVIIFI
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHH
MAITLVILGLLFPFMENIFMKASEAITTRVGVYDYSLKAYHEILRILGGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDQLAFKLAGLTVPLLALIAFLIPLLEKLTGSRDAPRILTLLGSLIAVFSSLIILISVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
TSGEVILYPFGGWPPHFGIVYEIDELNALIGAYIALAMLAVVAYSTWYGRHLDEPIWYYV
CCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LLTGLEIGMLGCVYTGDAFNLFVMLEVLSVSAYGLVAYHKNRAEAVEAAAKYALIGAVAT
HHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHECCCHHHHHHHHHHHHHHHHHHH
NLYFAALVIIYVTHGSLNMGLLSLIARQYSGAGEAVKYAGLIAVAFSLWVFTYKSALFPS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
HFWLPDAHPEAPTPVSATLSGVLVNVGVYAVMRFLYTIFGSDSYLAEFRAIVLATLFILG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
SLSGLIGALMMMMQKDVKRLLAYSTISHIGIIYIGLSIGFLSGVGEAVKIAMAGAIAHMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SHGIAKTMLFMASGVFIDAAGSRDLDELRGIGRNYPIASFAWLTGFLSLAGLLPFLGFYS
HHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KLLIYYGYTYSGFILGGVMIIVISALSLPGCFKAIYSVIFSIGREREKTNAGTAEVIIFI
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHH
MAITLVILGLLFPFMENIFMKASEAITTRVGVYDYSLKAYHEILRILGGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA