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The map label for this gene is malE [H]

Identifier: 218883716

GI number: 218883716

Start: 378836

End: 380383

Strand: Reverse

Name: malE [H]

Synonym: DKAM_0405

Alternate gene names: 218883716

Gene position: 380383-378836 (Counterclockwise)

Preceding gene: 218883717

Following gene: 218883715

Centisome position: 27.86

GC content: 45.8

Gene sequence:

>1548_bases
ATGACGTTAACAGCTATAACTAAAACCTCGGCCATCATAATTGTTATCATCGCAATCATAGCGGTGGCCGGTGGAGTACT
CCTATTGACTCAACAACCAGCCCAGCCACCATCAACAACCACTACTACAACAACCCAGACGTCCACCACAACAACTATGA
CAACCACCACTACCACAACTACTACGACAACCACTACACCGGTGGGCTCTACAATAACAATTGGTAATGTCACCATAAGG
GTGCCTGAGGAGTTTGCTAAGTTTGTTGAGAATGTCAAGACAGGTAATGTCAAGGTGACGATATACTTTGGGCATGCATT
AAACCCTGAGGAAAGAGACTCATTCATTAAAGTCATAGGGGCCTTTGAACAAGAATATCCAGGAATAGATGTCGTAGAGA
AGAGATATGGGGGAATGGGTGAGCTTCAAAGCGCGGTCATAGCTGCTGCATCGCTTCCACCATCACAAAGGGAAAGCCTT
GTTGGCCAGGCTCCCGACGTGTTTAACTGGGCCCATGACTGGATTGGTTGGTTTGCTGATTCCGGGTACCTTGTGTCGCT
TGAAGATTATATAGGATATGATGCAGTTGACGATATATCTCAGAACACACTTCCATCAGCTATGAGCTCTGTTACGTATC
TGGGTAAGACGTATGGCCTCCCATATGCTGGTGAGGCATTAGCACTCTATGTGAATACAGGGCTGGTTCCAAACCCGCCG
ACAACATTCAATGAGTTAAAGACCTTAATGGAGCAGTTCTATAAGCCGAGCGAGGGTAAATATGGGATAGCCGGTCAGAT
CGCGGGCCAATACCATACTAATCCCTGGGTTACAGCGTTCGGTGGTTTCTACTATGATGACGTGGGGAAGGAACTAGGCT
ACTTAAAGCCTGAGACAGCCGAGGGCCTCAAGTTCTTCGTGGCCAACATCCTACGGTACATGGATGTATCCGACCTCGGC
AACGATTATCAGAGGAGACTATTTGGTGAGGGTAAGACCCCATTCTATATATCTGGGCCATGGGATGTCAAGTACGCTGT
TGAAACATTAGGTGTCAACAACTTCACTGTTGTTCCACTACCATCGATTAATGATAGAATACCTAAGCCGTGGAGTGGAT
TTAGAACAATATATGTCTCGGTTATGGCTACGGCTGGAGGTAAGGAGAGAACATATGCTAGTATATTGTTTGCATTATAC
ATCGCCCTAGATGATAAAGCTATTCTAACACTTGTCAACGAGCTTGGATACATCCCAGTCAAGCCGAGTGTAGCTGATTA
TGTGAGGACAAACATAAATGCAAACCCGCTCTACAAGATAGTACTAGGATTCTATGAACAGCTAGGAAAAAGTGTTCCAA
TGCCGAAGGACGCGAATATGCAGAAGGTCTGGGGAGCCGATACGTATCTCCAAGCAATATGGAAGGCGTATTCAGATGCT
ATAAGCTCTGGTAAATCACCAGATGATGCTGTGCAAGCTGCGATCGCTCAGGTGGATAAGGCGCTCAGTGACGCCTATAA
CGATATATCTGCAAAGATAAAGAAATAA

Upstream 100 bases:

>100_bases
ACATTCTTTATTTAGCATTCTAAATGCAACCCATAGATTTTTACAAAGATTAAATATATAACCTTGGTTTACTTATAGGC
ATTATTAGGGGGTATACCTC

Downstream 100 bases:

>100_bases
TAGTTTAGGGATTAAGGACCGGTGGATGGGATGAGCTCTAAGAGGAAAATCCTTTTTCCCACACGCATCGCGCTTATCTT
GGTAATGCCGGCATTGATCT

Product: Cyclomaltodextrin binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTLTAITKTSAIIIVIIAIIAVAGGVLLLTQQPAQPPSTTTTTTTQTSTTTTMTTTTTTTTTTTTTPVGSTITIGNVTIR
VPEEFAKFVENVKTGNVKVTIYFGHALNPEERDSFIKVIGAFEQEYPGIDVVEKRYGGMGELQSAVIAAASLPPSQRESL
VGQAPDVFNWAHDWIGWFADSGYLVSLEDYIGYDAVDDISQNTLPSAMSSVTYLGKTYGLPYAGEALALYVNTGLVPNPP
TTFNELKTLMEQFYKPSEGKYGIAGQIAGQYHTNPWVTAFGGFYYDDVGKELGYLKPETAEGLKFFVANILRYMDVSDLG
NDYQRRLFGEGKTPFYISGPWDVKYAVETLGVNNFTVVPLPSINDRIPKPWSGFRTIYVSVMATAGGKERTYASILFALY
IALDDKAILTLVNELGYIPVKPSVADYVRTNINANPLYKIVLGFYEQLGKSVPMPKDANMQKVWGADTYLQAIWKAYSDA
ISSGKSPDDAVQAAIAQVDKALSDAYNDISAKIKK

Sequences:

>Translated_515_residues
MTLTAITKTSAIIIVIIAIIAVAGGVLLLTQQPAQPPSTTTTTTTQTSTTTTMTTTTTTTTTTTTTPVGSTITIGNVTIR
VPEEFAKFVENVKTGNVKVTIYFGHALNPEERDSFIKVIGAFEQEYPGIDVVEKRYGGMGELQSAVIAAASLPPSQRESL
VGQAPDVFNWAHDWIGWFADSGYLVSLEDYIGYDAVDDISQNTLPSAMSSVTYLGKTYGLPYAGEALALYVNTGLVPNPP
TTFNELKTLMEQFYKPSEGKYGIAGQIAGQYHTNPWVTAFGGFYYDDVGKELGYLKPETAEGLKFFVANILRYMDVSDLG
NDYQRRLFGEGKTPFYISGPWDVKYAVETLGVNNFTVVPLPSINDRIPKPWSGFRTIYVSVMATAGGKERTYASILFALY
IALDDKAILTLVNELGYIPVKPSVADYVRTNINANPLYKIVLGFYEQLGKSVPMPKDANMQKVWGADTYLQAIWKAYSDA
ISSGKSPDDAVQAAIAQVDKALSDAYNDISAKIKK
>Mature_514_residues
TLTAITKTSAIIIVIIAIIAVAGGVLLLTQQPAQPPSTTTTTTTQTSTTTTMTTTTTTTTTTTTTPVGSTITIGNVTIRV
PEEFAKFVENVKTGNVKVTIYFGHALNPEERDSFIKVIGAFEQEYPGIDVVEKRYGGMGELQSAVIAAASLPPSQRESLV
GQAPDVFNWAHDWIGWFADSGYLVSLEDYIGYDAVDDISQNTLPSAMSSVTYLGKTYGLPYAGEALALYVNTGLVPNPPT
TFNELKTLMEQFYKPSEGKYGIAGQIAGQYHTNPWVTAFGGFYYDDVGKELGYLKPETAEGLKFFVANILRYMDVSDLGN
DYQRRLFGEGKTPFYISGPWDVKYAVETLGVNNFTVVPLPSINDRIPKPWSGFRTIYVSVMATAGGKERTYASILFALYI
ALDDKAILTLVNELGYIPVKPSVADYVRTNINANPLYKIVLGFYEQLGKSVPMPKDANMQKVWGADTYLQAIWKAYSDAI
SSGKSPDDAVQAAIAQVDKALSDAYNDISAKIKK

Specific function: Involved in an abc transport system for maltotriose. Binds maltotriose much more tightly than maltose [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=349, Percent_Identity=26.9340974212034, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 56110; Mature: 55979

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTAITKTSAIIIVIIAIIAVAGGVLLLTQQPAQPPSTTTTTTTQTSTTTTMTTTTTTT
CEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEE
TTTTTTPVGSTITIGNVTIRVPEEFAKFVENVKTGNVKVTIYFGHALNPEERDSFIKVIG
EEEECCCCCCEEEECCEEEECCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
AFEQEYPGIDVVEKRYGGMGELQSAVIAAASLPPSQRESLVGQAPDVFNWAHDWIGWFAD
HHHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCC
SGYLVSLEDYIGYDAVDDISQNTLPSAMSSVTYLGKTYGLPYAGEALALYVNTGLVPNPP
CCEEEEEHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC
TTFNELKTLMEQFYKPSEGKYGIAGQIAGQYHTNPWVTAFGGFYYDDVGKELGYLKPETA
CCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHCCCCCCHH
EGLKFFVANILRYMDVSDLGNDYQRRLFGEGKTPFYISGPWDVKYAVETLGVNNFTVVPL
HHHHHHHHHHHHHHCHHHCCCHHHHHHHCCCCCCEEEECCCCCEEHHHHCCCCCEEEEEC
PSINDRIPKPWSGFRTIYVSVMATAGGKERTYASILFALYIALDDKAILTLVNELGYIPV
CCCCCCCCCCCCCHHHEEEEEHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
KPSVADYVRTNINANPLYKIVLGFYEQLGKSVPMPKDANMQKVWGADTYLQAIWKAYSDA
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
ISSGKSPDDAVQAAIAQVDKALSDAYNDISAKIKK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLTAITKTSAIIIVIIAIIAVAGGVLLLTQQPAQPPSTTTTTTTQTSTTTTMTTTTTTT
EEEEECCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEE
TTTTTTPVGSTITIGNVTIRVPEEFAKFVENVKTGNVKVTIYFGHALNPEERDSFIKVIG
EEEECCCCCCEEEECCEEEECCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
AFEQEYPGIDVVEKRYGGMGELQSAVIAAASLPPSQRESLVGQAPDVFNWAHDWIGWFAD
HHHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCC
SGYLVSLEDYIGYDAVDDISQNTLPSAMSSVTYLGKTYGLPYAGEALALYVNTGLVPNPP
CCEEEEEHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC
TTFNELKTLMEQFYKPSEGKYGIAGQIAGQYHTNPWVTAFGGFYYDDVGKELGYLKPETA
CCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHCCCCCCHH
EGLKFFVANILRYMDVSDLGNDYQRRLFGEGKTPFYISGPWDVKYAVETLGVNNFTVVPL
HHHHHHHHHHHHHHCHHHCCCHHHHHHHCCCCCCEEEECCCCCEEHHHHCCCCCEEEEEC
PSINDRIPKPWSGFRTIYVSVMATAGGKERTYASILFALYIALDDKAILTLVNELGYIPV
CCCCCCCCCCCCCHHHEEEEEHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
KPSVADYVRTNINANPLYKIVLGFYEQLGKSVPMPKDANMQKVWGADTYLQAIWKAYSDA
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
ISSGKSPDDAVQAAIAQVDKALSDAYNDISAKIKK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11162100 [H]