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The map label for this gene is malF [H]

Identifier: 218883715

GI number: 218883715

Start: 377372

End: 378709

Strand: Reverse

Name: malF [H]

Synonym: DKAM_0404

Alternate gene names: 218883715

Gene position: 378709-377372 (Counterclockwise)

Preceding gene: 218883716

Following gene: 218883714

Centisome position: 27.74

GC content: 43.05

Gene sequence:

>1338_bases
ATGATATTCTCCATAGGAATAGCATTTACAGATGCTACTCAGAAGAATATATCGCCTAGTCCAGAAGCCATAGACAACTG
GGAGAACGCGATCCGGTGTGCTATTGACCTTAGAGACAAGCCGGAGTACAAGGAGAAAGCCTCCGGCCTAGTGAATTCCA
CAGTAAGTAGCCTAATGGATGTATATGGCAACTTGACAGGGATAAAAAACTATATCGAGGCCGGTGGAGACTTCTTGAAT
ACGCCTCTAAGCTACTTAAGAGGGTTAGATCGATCTGTTAGGACATCAATGAGGGTGTCAGAAGAGTTCAAGATGTACTT
CAACTGCACTGAGCTAGGTTACCCCACAAGTATATCACCGATCTCTAAAGAAATGCTTGAGAAATTTGATGCATTAAGTA
CGTTGAGTGGAAGGCTTCAAAACCCAGGTGCATATGATAATAAGACCTACTATAATGAGGTTGTCACCGGATACAATATA
ACCATTGAGCTGGTAAACTACTTTAAGCGTCTTGAGACGGATTACGAGGGATATATGAATGAATTCATAGCGAGTGCCCA
GAATGAACTGGATTCACTACAGTTGAAGTTCATAGGGTTGGAGAACTTCAAGAAGCTATTCTATGACCCGAGATTCTATA
ACTCACTATATAAGACAATGCTCTTTGTTGCTACTAGTGTGCCACTCAAGGTTGGATTCGGCGTTCTACTCGCCTTATTT
TATTCCTCGGAGCTCCTCAGAGGCAGGAGAATAATGAGAGCCCTCATTATTGTTCCATGGGCAATGCCATTTCTACTCTC
AGCACTCACATGGAAGTTCCTGTTTATACCTAATGGCCAACTAGGCCAATTACTCGGGCTTAACATGAATAACAGGGAGT
GGGATGCATTCCTAGTGTACAACCTATTTGAGACGTGGCTTGCTTACCCATTCATAATGACTATAACACAAGGTGCTCTT
GGAGGAGTACCAAAAGAAGTCATAGAGGCATCATATATTGATGGTGCTAATGCCTTTACAAGACTAAGGAAAGTGATATT
TCCATTGATCAAGAAGCCCGTGCTGGTGGCATCGATCTCGACGACAGGTGCCTCGTTACAGGCATTCCTTGTACCGCTGA
CGCTTAATGGTGCAGGTCCCACTGGATACATTTGTGCACCGTTTGTCGGTTGCACAGCAGGGTATATGAATGAGATGCTG
ATAGTGTTCGGATATTATAGGGTCATAATTGACCAGGAATACGGATATGCTGCCTCAATCTACCTTGTTATCCTGGCAAT
AATACTGGTTTATGTAACTCTATGGCTTAAACTACTAATGGAGAAAGGTGGTAAGTAG

Upstream 100 bases:

>100_bases
TGGGATGAGCTCTAAGAGGAAAATCCTTTTTCCCACACGCATCGCGCTTATCTTGGTAATGCCGGCATTGATCTTGTATT
TATTTTTTAATACATGGCCA

Downstream 100 bases:

>100_bases
TATGGTTTCAGTCTCTCTAACTATACTTAAAATAACCCTTACAATTATAGGGGTCTTTATAGCCGTCGCCATCGCTTATC
CACTAATATACATAGTGGCA

Product: sugar ABC transporter permease

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MIFSIGIAFTDATQKNISPSPEAIDNWENAIRCAIDLRDKPEYKEKASGLVNSTVSSLMDVYGNLTGIKNYIEAGGDFLN
TPLSYLRGLDRSVRTSMRVSEEFKMYFNCTELGYPTSISPISKEMLEKFDALSTLSGRLQNPGAYDNKTYYNEVVTGYNI
TIELVNYFKRLETDYEGYMNEFIASAQNELDSLQLKFIGLENFKKLFYDPRFYNSLYKTMLFVATSVPLKVGFGVLLALF
YSSELLRGRRIMRALIIVPWAMPFLLSALTWKFLFIPNGQLGQLLGLNMNNREWDAFLVYNLFETWLAYPFIMTITQGAL
GGVPKEVIEASYIDGANAFTRLRKVIFPLIKKPVLVASISTTGASLQAFLVPLTLNGAGPTGYICAPFVGCTAGYMNEML
IVFGYYRVIIDQEYGYAASIYLVILAIILVYVTLWLKLLMEKGGK

Sequences:

>Translated_445_residues
MIFSIGIAFTDATQKNISPSPEAIDNWENAIRCAIDLRDKPEYKEKASGLVNSTVSSLMDVYGNLTGIKNYIEAGGDFLN
TPLSYLRGLDRSVRTSMRVSEEFKMYFNCTELGYPTSISPISKEMLEKFDALSTLSGRLQNPGAYDNKTYYNEVVTGYNI
TIELVNYFKRLETDYEGYMNEFIASAQNELDSLQLKFIGLENFKKLFYDPRFYNSLYKTMLFVATSVPLKVGFGVLLALF
YSSELLRGRRIMRALIIVPWAMPFLLSALTWKFLFIPNGQLGQLLGLNMNNREWDAFLVYNLFETWLAYPFIMTITQGAL
GGVPKEVIEASYIDGANAFTRLRKVIFPLIKKPVLVASISTTGASLQAFLVPLTLNGAGPTGYICAPFVGCTAGYMNEML
IVFGYYRVIIDQEYGYAASIYLVILAIILVYVTLWLKLLMEKGGK
>Mature_445_residues
MIFSIGIAFTDATQKNISPSPEAIDNWENAIRCAIDLRDKPEYKEKASGLVNSTVSSLMDVYGNLTGIKNYIEAGGDFLN
TPLSYLRGLDRSVRTSMRVSEEFKMYFNCTELGYPTSISPISKEMLEKFDALSTLSGRLQNPGAYDNKTYYNEVVTGYNI
TIELVNYFKRLETDYEGYMNEFIASAQNELDSLQLKFIGLENFKKLFYDPRFYNSLYKTMLFVATSVPLKVGFGVLLALF
YSSELLRGRRIMRALIIVPWAMPFLLSALTWKFLFIPNGQLGQLLGLNMNNREWDAFLVYNLFETWLAYPFIMTITQGAL
GGVPKEVIEASYIDGANAFTRLRKVIFPLIKKPVLVASISTTGASLQAFLVPLTLNGAGPTGYICAPFVGCTAGYMNEML
IVFGYYRVIIDQEYGYAASIYLVILAIILVYVTLWLKLLMEKGGK

Specific function: Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790465, Length=247, Percent_Identity=29.9595141700405, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787570, Length=179, Percent_Identity=29.0502793296089, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789861, Length=164, Percent_Identity=30.4878048780488, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 50104; Mature: 50104

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSIGIAFTDATQKNISPSPEAIDNWENAIRCAIDLRDKPEYKEKASGLVNSTVSSLMD
CEEEEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VYGNLTGIKNYIEAGGDFLNTPLSYLRGLDRSVRTSMRVSEEFKMYFNCTELGYPTSISP
HHHCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEHHCCCCCCCCH
ISKEMLEKFDALSTLSGRLQNPGAYDNKTYYNEVVTGYNITIELVNYFKRLETDYEGYMN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHH
EFIASAQNELDSLQLKFIGLENFKKLFYDPRFYNSLYKTMLFVATSVPLKVGFGVLLALF
HHHHHHHHHHHHHEEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YSSELLRGRRIMRALIIVPWAMPFLLSALTWKFLFIPNGQLGQLLGLNMNNREWDAFLVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHCCCCCCCCCHHHHHHH
NLFETWLAYPFIMTITQGALGGVPKEVIEASYIDGANAFTRLRKVIFPLIKKPVLVASIS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEC
TTGASLQAFLVPLTLNGAGPTGYICAPFVGCTAGYMNEMLIVFGYYRVIIDQEYGYAASI
CCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHH
YLVILAIILVYVTLWLKLLMEKGGK
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIFSIGIAFTDATQKNISPSPEAIDNWENAIRCAIDLRDKPEYKEKASGLVNSTVSSLMD
CEEEEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VYGNLTGIKNYIEAGGDFLNTPLSYLRGLDRSVRTSMRVSEEFKMYFNCTELGYPTSISP
HHHCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEHHCCCCCCCCH
ISKEMLEKFDALSTLSGRLQNPGAYDNKTYYNEVVTGYNITIELVNYFKRLETDYEGYMN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHH
EFIASAQNELDSLQLKFIGLENFKKLFYDPRFYNSLYKTMLFVATSVPLKVGFGVLLALF
HHHHHHHHHHHHHEEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YSSELLRGRRIMRALIIVPWAMPFLLSALTWKFLFIPNGQLGQLLGLNMNNREWDAFLVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHCCCCCCCCCHHHHHHH
NLFETWLAYPFIMTITQGALGGVPKEVIEASYIDGANAFTRLRKVIFPLIKKPVLVASIS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEC
TTGASLQAFLVPLTLNGAGPTGYICAPFVGCTAGYMNEMLIVFGYYRVIIDQEYGYAASI
CCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHH
YLVILAIILVYVTLWLKLLMEKGGK
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]