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The map label for this gene is panB [H]

Identifier: 218883652

GI number: 218883652

Start: 314220

End: 315035

Strand: Reverse

Name: panB [H]

Synonym: DKAM_0341

Alternate gene names: 218883652

Gene position: 315035-314220 (Counterclockwise)

Preceding gene: 218883653

Following gene: 218883649

Centisome position: 23.08

GC content: 48.65

Gene sequence:

>816_bases
GTGTCAGCCTTGAATACAAGGGTAACGGTTAGAGACATAGTTAAGATGAAGGGGAAAGAGAAGATAGCAATGGTTACAGC
CTACGATTATACAACGGCTAAACTGGTTGATCAAGCAGGCATAGATATAATCCTCGTCGGTGACAGCGTTGGAATGGTTG
TCCATGGATTAGATACAACGATACCTGTAACAATGGATATGATGCTACTACACGTATCCAGCGTTGCAAGGGCAAGGCCC
CGCGCCCTTGTGGTAGGGGACATGCCATTCCTCAGCTATGAAACCAGTATCGAGGATGCAGTGAGGCATGCAGGCCTCCT
CATGAAGGCGGGCGCTGACGCCGTTAAGCTGGAGGGTGGATCCGAGTTAAGCGATGTGGTGAAAGTCCTTGTGAGAGCCG
GCATACCTGTCATGGGGCACATCGGGTTGACCCCTCAGAGATCATTCCTTATAGGTGGTTATAGGAAACGTGGTGTGAGC
GAGAGGGAGCGGGAGAAGATACTAGAGGATGCGAGGGAACTAGAGAGAGCCGGAGTGTTTTCAATGGTGATAGAGTATAC
GGCTGCTGATGTAGCCGACGAGGTAACAAGAGAGGTCAGTGTACCCACTATCTGCATTGGAAGCGGGCCTTTCTGTGATG
GACAGGTATTGGTTATTCATGACCTACTGGGATTAACAGAGAATCCACCTCCATTCGCTAAAGCCTATGCCGACTTAAGA
AGCATCATAGTTAACGCTGTCAAGGAATATGTTAGGGAAGTGAAGAACGGGGTTTTCCCAGAGGAAAAACACTACTTCTA
CTCTAAAAAACAGTAG

Upstream 100 bases:

>100_bases
CTCATCGAGGAAAGCAATAAGTCTAAGAATAGTGTCCAATAGCCATGAATTATATCCACTGGCTTAATTTAAATAGATCA
GAACCCAATAGTGAAACCCG

Downstream 100 bases:

>100_bases
GACTGCCTGCTTATTGATAAACTTTCAAGTGCTCCAGCTATTATGGATGCTTGGATGTGCTTTCCCACCGCAATGACGAC
CAAGCGGTACCCATCATGAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSALNTRVTVRDIVKMKGKEKIAMVTAYDYTTAKLVDQAGIDIILVGDSVGMVVHGLDTTIPVTMDMMLLHVSSVARARP
RALVVGDMPFLSYETSIEDAVRHAGLLMKAGADAVKLEGGSELSDVVKVLVRAGIPVMGHIGLTPQRSFLIGGYRKRGVS
EREREKILEDARELERAGVFSMVIEYTAADVADEVTREVSVPTICIGSGPFCDGQVLVIHDLLGLTENPPPFAKAYADLR
SIIVNAVKEYVREVKNGVFPEEKHYFYSKKQ

Sequences:

>Translated_271_residues
MSALNTRVTVRDIVKMKGKEKIAMVTAYDYTTAKLVDQAGIDIILVGDSVGMVVHGLDTTIPVTMDMMLLHVSSVARARP
RALVVGDMPFLSYETSIEDAVRHAGLLMKAGADAVKLEGGSELSDVVKVLVRAGIPVMGHIGLTPQRSFLIGGYRKRGVS
EREREKILEDARELERAGVFSMVIEYTAADVADEVTREVSVPTICIGSGPFCDGQVLVIHDLLGLTENPPPFAKAYADLR
SIIVNAVKEYVREVKNGVFPEEKHYFYSKKQ
>Mature_270_residues
SALNTRVTVRDIVKMKGKEKIAMVTAYDYTTAKLVDQAGIDIILVGDSVGMVVHGLDTTIPVTMDMMLLHVSSVARARPR
ALVVGDMPFLSYETSIEDAVRHAGLLMKAGADAVKLEGGSELSDVVKVLVRAGIPVMGHIGLTPQRSFLIGGYRKRGVSE
REREKILEDARELERAGVFSMVIEYTAADVADEVTREVSVPTICIGSGPFCDGQVLVIHDLLGLTENPPPFAKAYADLRS
IIVNAVKEYVREVKNGVFPEEKHYFYSKKQ

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=250, Percent_Identity=41.6, Blast_Score=210, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6319653, Length=259, Percent_Identity=38.996138996139, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 29637; Mature: 29506

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALNTRVTVRDIVKMKGKEKIAMVTAYDYTTAKLVDQAGIDIILVGDSVGMVVHGLDTT
CCCCCHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHCCCEEEEECCCCCEEEECCCCC
IPVTMDMMLLHVSSVARARPRALVVGDMPFLSYETSIEDAVRHAGLLMKAGADAVKLEGG
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHCCHHHHHHHHCHHEECCCCEEEECCC
SELSDVVKVLVRAGIPVMGHIGLTPQRSFLIGGYRKRGVSEREREKILEDARELERAGVF
CCHHHHHHHHHHCCCCEEECCCCCCCCHHEECCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SMVIEYTAADVADEVTREVSVPTICIGSGPFCDGQVLVIHDLLGLTENPPPFAKAYADLR
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHH
SIIVNAVKEYVREVKNGVFPEEKHYFYSKKQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SALNTRVTVRDIVKMKGKEKIAMVTAYDYTTAKLVDQAGIDIILVGDSVGMVVHGLDTT
CCCCHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHCCCEEEEECCCCCEEEECCCCC
IPVTMDMMLLHVSSVARARPRALVVGDMPFLSYETSIEDAVRHAGLLMKAGADAVKLEGG
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHCCHHHHHHHHCHHEECCCCEEEECCC
SELSDVVKVLVRAGIPVMGHIGLTPQRSFLIGGYRKRGVSEREREKILEDARELERAGVF
CCHHHHHHHHHHCCCCEEECCCCCCCCHHEECCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SMVIEYTAADVADEVTREVSVPTICIGSGPFCDGQVLVIHDLLGLTENPPPFAKAYADLR
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHH
SIIVNAVKEYVREVKNGVFPEEKHYFYSKKQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA