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The map label for this gene is lig [H]

Identifier: 218883590

GI number: 218883590

Start: 258025

End: 259857

Strand: Reverse

Name: lig [H]

Synonym: DKAM_0279

Alternate gene names: 218883590

Gene position: 259857-258025 (Counterclockwise)

Preceding gene: 218883592

Following gene: 218883589

Centisome position: 19.03

GC content: 49.1

Gene sequence:

>1833_bases
ATGAGTATCGGGCAGGCAGATATCCCGGTAAGGCTTATCGCCGATGTATTCGAGCGTATAGAGGTAATAACCTCAAGGAT
CCAGATGACCGCGCTGCTGGTTACTCTATTCAAGCAGACCCCACCAGAGATCATTGATCGCGTTGTCTATCTAATACAGG
GCAGGCTATGGCCTGACTGGAAAGAACTCCCGGAGCTCGGGGTAGGCGAGAAGCTACTGGTTAAAGCTATAGCTCTGGCT
ACGAACAGTAGTGAGGCACAGGTTGAAGACCTAGCCAAAAGCCTCGGCGACCTGGGTAAAGTAGCCGAGCAATTAAAATC
GAGGATCAGCGGTAGACAGCAGGGTGTAACATTATTCAGCTTCACTCAGCAACAACCGGAATTAACGGTTAACAAAGTAT
ATGATACACTAGTAAGGGTGGCGATGGCTCAAGGCGAGGGAAGCAAGGATCTGAAAATAAGGCTTTTAGCCGGGCTCCTC
AGAGAGGCCTCACCCAAGGAGGCCAAGTTCATCGTGAGGTTTGCCGAAGGCAGGCTAAGGGTTGGAATAGGCGAGGCAAC
AGTCATGGATGCCCTAGCAATAACTTATGGGGGTGGGGCGGCTGCTAGACAGGTTATCGAGAGAGCGTATAATCTCAGAG
CGGATCTCGGGGAGGTAGCCAAGATACTTGCCTTGAATGGGTTGGAAGCCCTCAGGAATGTTAGGCCGGAGGTCGGGGTC
CCTATAAGGCCAATGCTGGCTGAGAGGCTCAGCGACCCCAAGGAGATACTTGAAAAAGTGGGTGGGGATGCATTCGTCGA
GTACAAGTATGATGGTGAGAGAGCCCAGATACATAAGAAGGATGATAAGGTATGGATTTTTTCCAGGAGGCTTGAAAACA
TAACCCACCAATACCCAGATGTCATTGAATTAGCTAGGGATCACTTAAAGGCTAGAGAAGTCATCGTGGAGGGTGAGATA
GTAGCATACGACCTGGATACAGGTGAGTTGAAGCCTTTCCAAGAGCTTATGCATAGAAAGAGAAAGCACGAGGTCCGCAC
AGCACTTAAGGAATACCCTGTGAAGGTCTTCCTGTTTGATCTATTATACGCGGATGGACGGGACTATACCATAGAACCCC
TCCCAGTTAGGAGGGGTAAACTATTGGAGATCGTTGATAGAACAGATGAGTTCACAGTGGCCGAGTATATAAGGACGAGC
GATGTAAAAGAGCTTGAAAAATTCTTCTTGAAAGCCATAAGCGATGGAGCGGAGGGTGTTATGGTTAAAGCTTTACATGA
TGGCTCAATATATCAGGCTGGCACAAGGGGCTGGCTATGGATAAAGTTCAAGCGTGACTACAGGAGCGAAATGATCGATA
CAGTAGACCTAGTCGTCGTAGGAGCCTTCTATGGGAAGGGGAGGAGAGGTGGGAAGTACGGTGCACTACTCATGGCTGCC
TATAACGATGAGAAAGACGTCTTCGAGACAGTATGCAAGGTGGGCTCCGGATTCAAGGACGAAGACCTAGACAAACTCCC
AGAGATACTGAAGCCGTATATAAGAGATAGGAAGTCGCCTCGCGTGGTAGCCGAGATGGAGCCCGATGTATGGGTTGAGC
CGGCACTTGTCGCTGAGATTATTGGGGCTGAATTAACTCTTTCACCAATACATACATGCGCCTACGGCAGGATTAAGAGC
GATGCAGGCTTATCCATAAGGTTCCCAAGATTCATAAGGTGGAGACCTGACAAGAGGCCTGAGGATGCTACAACTAGTAA
CGAGCTCGTTGAAATGTATAATCAGCAGTTAAAGAAGATAGCTGGAGAGAGTAAGCCGGTTGAGGAAGCCTAG

Upstream 100 bases:

>100_bases
CATCACCATATTATGGTTTATTTCAACAGGGGTTGAAAAACCTGGATTGTTGCTTTATATTGGAGTAAACCTAATAAATA
TACCTGGGATGGTGCACTCC

Downstream 100 bases:

>100_bases
CCACTCCATTGTACTTAGCGGGGGCAGGGTGCCTTAATGGTCTCTATAATAGTGTTCACTGGTCCTGCAGGCTCTGGTAA
GACAAGCCTGGTTAAAGCGT

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MSIGQADIPVRLIADVFERIEVITSRIQMTALLVTLFKQTPPEIIDRVVYLIQGRLWPDWKELPELGVGEKLLVKAIALA
TNSSEAQVEDLAKSLGDLGKVAEQLKSRISGRQQGVTLFSFTQQQPELTVNKVYDTLVRVAMAQGEGSKDLKIRLLAGLL
REASPKEAKFIVRFAEGRLRVGIGEATVMDALAITYGGGAAARQVIERAYNLRADLGEVAKILALNGLEALRNVRPEVGV
PIRPMLAERLSDPKEILEKVGGDAFVEYKYDGERAQIHKKDDKVWIFSRRLENITHQYPDVIELARDHLKAREVIVEGEI
VAYDLDTGELKPFQELMHRKRKHEVRTALKEYPVKVFLFDLLYADGRDYTIEPLPVRRGKLLEIVDRTDEFTVAEYIRTS
DVKELEKFFLKAISDGAEGVMVKALHDGSIYQAGTRGWLWIKFKRDYRSEMIDTVDLVVVGAFYGKGRRGGKYGALLMAA
YNDEKDVFETVCKVGSGFKDEDLDKLPEILKPYIRDRKSPRVVAEMEPDVWVEPALVAEIIGAELTLSPIHTCAYGRIKS
DAGLSIRFPRFIRWRPDKRPEDATTSNELVEMYNQQLKKIAGESKPVEEA

Sequences:

>Translated_610_residues
MSIGQADIPVRLIADVFERIEVITSRIQMTALLVTLFKQTPPEIIDRVVYLIQGRLWPDWKELPELGVGEKLLVKAIALA
TNSSEAQVEDLAKSLGDLGKVAEQLKSRISGRQQGVTLFSFTQQQPELTVNKVYDTLVRVAMAQGEGSKDLKIRLLAGLL
REASPKEAKFIVRFAEGRLRVGIGEATVMDALAITYGGGAAARQVIERAYNLRADLGEVAKILALNGLEALRNVRPEVGV
PIRPMLAERLSDPKEILEKVGGDAFVEYKYDGERAQIHKKDDKVWIFSRRLENITHQYPDVIELARDHLKAREVIVEGEI
VAYDLDTGELKPFQELMHRKRKHEVRTALKEYPVKVFLFDLLYADGRDYTIEPLPVRRGKLLEIVDRTDEFTVAEYIRTS
DVKELEKFFLKAISDGAEGVMVKALHDGSIYQAGTRGWLWIKFKRDYRSEMIDTVDLVVVGAFYGKGRRGGKYGALLMAA
YNDEKDVFETVCKVGSGFKDEDLDKLPEILKPYIRDRKSPRVVAEMEPDVWVEPALVAEIIGAELTLSPIHTCAYGRIKS
DAGLSIRFPRFIRWRPDKRPEDATTSNELVEMYNQQLKKIAGESKPVEEA
>Mature_609_residues
SIGQADIPVRLIADVFERIEVITSRIQMTALLVTLFKQTPPEIIDRVVYLIQGRLWPDWKELPELGVGEKLLVKAIALAT
NSSEAQVEDLAKSLGDLGKVAEQLKSRISGRQQGVTLFSFTQQQPELTVNKVYDTLVRVAMAQGEGSKDLKIRLLAGLLR
EASPKEAKFIVRFAEGRLRVGIGEATVMDALAITYGGGAAARQVIERAYNLRADLGEVAKILALNGLEALRNVRPEVGVP
IRPMLAERLSDPKEILEKVGGDAFVEYKYDGERAQIHKKDDKVWIFSRRLENITHQYPDVIELARDHLKAREVIVEGEIV
AYDLDTGELKPFQELMHRKRKHEVRTALKEYPVKVFLFDLLYADGRDYTIEPLPVRRGKLLEIVDRTDEFTVAEYIRTSD
VKELEKFFLKAISDGAEGVMVKALHDGSIYQAGTRGWLWIKFKRDYRSEMIDTVDLVVVGAFYGKGRRGGKYGALLMAAY
NDEKDVFETVCKVGSGFKDEDLDKLPEILKPYIRDRKSPRVVAEMEPDVWVEPALVAEIIGAELTLSPIHTCAYGRIKSD
AGLSIRFPRFIRWRPDKRPEDATTSNELVEMYNQQLKKIAGESKPVEEA

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=627, Percent_Identity=35.725677830941, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI73747829, Length=495, Percent_Identity=30.7070707070707, Blast_Score=173, Evalue=5e-43,
Organism=Homo sapiens, GI73747844, Length=495, Percent_Identity=30.7070707070707, Blast_Score=173, Evalue=5e-43,
Organism=Homo sapiens, GI46255052, Length=517, Percent_Identity=26.6924564796905, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI23199993, Length=517, Percent_Identity=26.6924564796905, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI148539894, Length=517, Percent_Identity=26.6924564796905, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71985269, Length=618, Percent_Identity=34.6278317152104, Blast_Score=328, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI71985278, Length=193, Percent_Identity=30.0518134715026, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320038, Length=625, Percent_Identity=35.68, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6324578, Length=245, Percent_Identity=29.7959183673469, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24762424, Length=618, Percent_Identity=35.9223300970874, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI85815841, Length=552, Percent_Identity=27.8985507246377, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24641829, Length=493, Percent_Identity=26.369168356998, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 68827; Mature: 68696

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS00333 DNA_LIGASE_A2 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIGQADIPVRLIADVFERIEVITSRIQMTALLVTLFKQTPPEIIDRVVYLIQGRLWPDW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCH
KELPELGVGEKLLVKAIALATNSSEAQVEDLAKSLGDLGKVAEQLKSRISGRQQGVTLFS
HHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEE
FTQQQPELTVNKVYDTLVRVAMAQGEGSKDLKIRLLAGLLREASPKEAKFIVRFAEGRLR
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHEEEEEECCEEE
VGIGEATVMDALAITYGGGAAARQVIERAYNLRADLGEVAKILALNGLEALRNVRPEVGV
EECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
PIRPMLAERLSDPKEILEKVGGDAFVEYKYDGERAQIHKKDDKVWIFSRRLENITHQYPD
CCHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCHHEEECCCCCEEEHHHHHHHHHHCCHH
VIELARDHLKAREVIVEGEIVAYDLDTGELKPFQELMHRKRKHEVRTALKEYPVKVFLFD
HHHHHHHHHHHHHEEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLYADGRDYTIEPLPVRRGKLLEIVDRTDEFTVAEYIRTSDVKELEKFFLKAISDGAEGV
HHHCCCCCCEECCCCCCCCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCE
MVKALHDGSIYQAGTRGWLWIKFKRDYRSEMIDTVDLVVVGAFYGKGRRGGKYGALLMAA
EEEEEECCCEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
YNDEKDVFETVCKVGSGFKDEDLDKLPEILKPYIRDRKSPRVVAEMEPDVWVEPALVAEI
ECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
IGAELTLSPIHTCAYGRIKSDAGLSIRFPRFIRWRPDKRPEDATTSNELVEMYNQQLKKI
HCCCCEECHHHHHHCCCCCCCCCCEEECCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHH
AGESKPVEEA
CCCCCCCCCC
>Mature Secondary Structure 
SIGQADIPVRLIADVFERIEVITSRIQMTALLVTLFKQTPPEIIDRVVYLIQGRLWPDW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCH
KELPELGVGEKLLVKAIALATNSSEAQVEDLAKSLGDLGKVAEQLKSRISGRQQGVTLFS
HHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEEE
FTQQQPELTVNKVYDTLVRVAMAQGEGSKDLKIRLLAGLLREASPKEAKFIVRFAEGRLR
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHEEEEEECCEEE
VGIGEATVMDALAITYGGGAAARQVIERAYNLRADLGEVAKILALNGLEALRNVRPEVGV
EECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
PIRPMLAERLSDPKEILEKVGGDAFVEYKYDGERAQIHKKDDKVWIFSRRLENITHQYPD
CCHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCHHEEECCCCCEEEHHHHHHHHHHCCHH
VIELARDHLKAREVIVEGEIVAYDLDTGELKPFQELMHRKRKHEVRTALKEYPVKVFLFD
HHHHHHHHHHHHHEEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLYADGRDYTIEPLPVRRGKLLEIVDRTDEFTVAEYIRTSDVKELEKFFLKAISDGAEGV
HHHCCCCCCEECCCCCCCCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCE
MVKALHDGSIYQAGTRGWLWIKFKRDYRSEMIDTVDLVVVGAFYGKGRRGGKYGALLMAA
EEEEEECCCEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
YNDEKDVFETVCKVGSGFKDEDLDKLPEILKPYIRDRKSPRVVAEMEPDVWVEPALVAEI
ECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
IGAELTLSPIHTCAYGRIKSDAGLSIRFPRFIRWRPDKRPEDATTSNELVEMYNQQLKKI
HCCCCEECHHHHHHCCCCCCCCCCEEECCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHH
AGESKPVEEA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA