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The map label for this gene is rnhB

Identifier: 218710304

GI number: 218710304

Start: 2507539

End: 2508159

Strand: Reverse

Name: rnhB

Synonym: VS_2338

Alternate gene names: 218710304

Gene position: 2508159-2507539 (Counterclockwise)

Preceding gene: 218710305

Following gene: 218710303

Centisome position: 76.02

GC content: 45.89

Gene sequence:

>621_bases
ATGGCAGTAAAAGAGAAAAAAGAATTTCCTCCTTTTGAGTATCCTCAAGGCTACCAGTTATTCGCTGGTGTTGATGAAGT
GGGGCGTGGGCCGTTAGTTGGTGATGTAGTTACCGCCGCGGTTATCCTCGATCCGAATAACCCAATCGAAGGCTTGAACG
ATTCCAAAAAACTGTCTGAGAAAAAGCGCCTTGCTCTGCTTCCTGAAATTAAAGAGAAAGCCTTGGCATGGTCTGTGGGT
CGTTGTTCCCCACAAGAAATTGATGAGTTGAATATCTTACAAGCGACTATGGTAGCTATGCAGCGTGCTATTGCCGGGCT
GAGTGTTCAACCTGATATGGCGCTAATAGATGGAAACCGAGTCCCTGAGCTACCGATGGCCGGTCTGGCTATTGTGAAAG
GGGATTTACGGGTGGCTGAAATCAGCGCAGCGTCTATCATCGCTAAAGTGGTTCGTGACCAAGAGATGGAAGAGCTTGAT
AAGCTTCATCCAGAGTTTGGTTTTGCTAAACATAAAGGTTACCCGACCAAAGCGCATTTTGAAGCGATTGAAAAACATGG
CGTAACCGAGCATTACCGTAAAAGCTTTAAGCCAGTAAAACGTATTCTTGGCATAGATTAG

Upstream 100 bases:

>100_bases
GGCAGCTAACGCAGTTCTGAAATTGATCGGTCGAGAGTGTGTTGAATCATAAAGTGCTTCTTTCAAAAGCATTTGCTTAA
AAGTTACAAAGAGAATTGTC

Downstream 100 bases:

>100_bases
AGCGAATTGAATAAGCTCTATATAGATTAGAGCTTTACGTAAACTAGAGCTTAGCATCGAATAAAGTTGCTTAGTGACGT
TAGAAAAAAATAGAATACAC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MAVKEKKEFPPFEYPQGYQLFAGVDEVGRGPLVGDVVTAAVILDPNNPIEGLNDSKKLSEKKRLALLPEIKEKALAWSVG
RCSPQEIDELNILQATMVAMQRAIAGLSVQPDMALIDGNRVPELPMAGLAIVKGDLRVAEISAASIIAKVVRDQEMEELD
KLHPEFGFAKHKGYPTKAHFEAIEKHGVTEHYRKSFKPVKRILGID

Sequences:

>Translated_206_residues
MAVKEKKEFPPFEYPQGYQLFAGVDEVGRGPLVGDVVTAAVILDPNNPIEGLNDSKKLSEKKRLALLPEIKEKALAWSVG
RCSPQEIDELNILQATMVAMQRAIAGLSVQPDMALIDGNRVPELPMAGLAIVKGDLRVAEISAASIIAKVVRDQEMEELD
KLHPEFGFAKHKGYPTKAHFEAIEKHGVTEHYRKSFKPVKRILGID
>Mature_205_residues
AVKEKKEFPPFEYPQGYQLFAGVDEVGRGPLVGDVVTAAVILDPNNPIEGLNDSKKLSEKKRLALLPEIKEKALAWSVGR
CSPQEIDELNILQATMVAMQRAIAGLSVQPDMALIDGNRVPELPMAGLAIVKGDLRVAEISAASIIAKVVRDQEMEELDK
LHPEFGFAKHKGYPTKAHFEAIEKHGVTEHYRKSFKPVKRILGID

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=194, Percent_Identity=70.1030927835051, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_VIBSL (B7VIQ4)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417925.1
- GeneID:   7161879
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2338
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22720; Mature: 22588

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKEKKEFPPFEYPQGYQLFAGVDEVGRGPLVGDVVTAAVILDPNNPIEGLNDSKKLSE
CCCCCCCCCCCCCCCCCCEEEECHHHHCCCCCHHHHHEEEEEECCCCCCCCCCCHHHHHH
KKRLALLPEIKEKALAWSVGRCSPQEIDELNILQATMVAMQRAIAGLSVQPDMALIDGNR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
VPELPMAGLAIVKGDLRVAEISAASIIAKVVRDQEMEELDKLHPEFGFAKHKGYPTKAHF
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHH
EAIEKHGVTEHYRKSFKPVKRILGID
HHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVKEKKEFPPFEYPQGYQLFAGVDEVGRGPLVGDVVTAAVILDPNNPIEGLNDSKKLSE
CCCCCCCCCCCCCCCCCEEEECHHHHCCCCCHHHHHEEEEEECCCCCCCCCCCHHHHHH
KKRLALLPEIKEKALAWSVGRCSPQEIDELNILQATMVAMQRAIAGLSVQPDMALIDGNR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
VPELPMAGLAIVKGDLRVAEISAASIIAKVVRDQEMEELDKLHPEFGFAKHKGYPTKAHF
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHH
EAIEKHGVTEHYRKSFKPVKRILGID
HHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA