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The map label for this gene is tilS

Identifier: 218710301

GI number: 218710301

Start: 2501410

End: 2502780

Strand: Reverse

Name: tilS

Synonym: VS_2335

Alternate gene names: 218710301

Gene position: 2502780-2501410 (Counterclockwise)

Preceding gene: 218710302

Following gene: 218710300

Centisome position: 75.86

GC content: 45.66

Gene sequence:

>1371_bases
ATGACTCACTTAATCGAAACCTTCACATCTGTACTTCATCAAAGCGCACTCAAGCCTAGTCGATTGATCGTGGCTTTCAG
TGGCGGTGTTGATTCTCGAGTTTTGTTAGAGTTAGCAGCGCAGTACGCGCAAACGCATGGCATTGAGTGCTGCGCGGTGC
ACGTTCATCACGGTCTCAGTAAGAATGCTGATCTTTGGGCTGAGCAATGCCAAACATGGTGTGATGCTTTGTCGGTTTCG
TTAGCAGTAGAAAGGGTCTCGTTGGATATCAACTGTGGTGAGAGCGTTGAAAAGCTTGCTCGAGATGCTCGATACCAAGC
TTTTCAGCAGCACATCAGACAGGGCGATGTGCTTGTTACGGGTCAACACATTGACGATCAACTTGAAACTTTCTTGCTGG
CATTAAAACGTGGCAGTGGTCCGAAAGGACTCTCTTCAATGGCGAAAGTGATGTCGTTTGGCGAAGCGTTTATTGTGAGA
CCTCTGCTGTCTGTGACTCGATTAGACATCGAAGCGTCGGCTCACGATATGGGGTTAACTTGGGTCGAAGATGAGAGTAA
TCAGGATCTGCGCTTTGATCGAAATTTCATTCGTCATCAAGTCACTCCGACGTTGACAGAGCGCTGGCCTAGCTTTCGAG
AATCGGTCTGTCGCAGTGCTCAATTATGTGCAGAACAAGAGTCGCTACTCGACGAACTGCTTGAGTCTCACTTACAACAA
GCTTTGGGAGGCTGTAACAGTAAGAGTAACAGTAAGAGTAACAGTAAGAGCCAGAGCTTAAGCATTGATTCGTTATCTCA
GCATTCTGATTTGTTACGAGCACGTTTGATCAGAATGTGGCTCAGCCATTGCAATCAACCGATGCCAAGCCAAAAGCAGC
TAAAACTTATTTGGGATGAAGTGGCTTGTGCTCAGGCAGATGCCAATCCTAAGCTGGTGTTGAATGATGTTGAGATTAGG
CGCTTTAATAATCAGCTCTATTTGGTACAAGACACCAAAGATTTGTCGAGCTGGAAAAGTGAAATCTTGATTGATGAGAA
TTTATTATTGCCTGATGGTTTAGGTGAGATCCATTTAAAAGCGGTGTCGAGTGGTAGTGCATCTCATAACCGAGATGTAC
AACGTTTTTCCCTTACTAAAGCCAACGGAACCTTACGAGTGATCTTTAACCCTGAAGGAGTGTCGGCTCACCCAGTTGGT
CGTGGTCATAGTCGAAAACTCAAGAAGCTTTTTCAGGAATACCAAGTACCCAGCTGGTTAAGACGTAGAACGCCGATATT
AATGGATGGCGATCGAGTGATCGCTGTTTTAGGCCTATTCGTAGATAAAAACTACGAAGGTCAAGATTGTGAAGCGCTTT
GGAGCAAGTAG

Upstream 100 bases:

>100_bases
TTTCCGTAACAAGTTGATGAAAGGGTTGGACTCAGTGCCAACCCTTTTTTATTATTAAAACATTCTCCTTTCTCATTAGT
ACACACGGCTCACTAACCCC

Downstream 100 bases:

>100_bases
AAAAAGTTTGTGTCACAATTCACCGTCACTTTAATAAAAATAATTAATGGAATATGCAATGAAGAAAATTACACTAGGAT
TAGTTCTTGGATTTGGACTA

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTHLIETFTSVLHQSALKPSRLIVAFSGGVDSRVLLELAAQYAQTHGIECCAVHVHHGLSKNADLWAEQCQTWCDALSVS
LAVERVSLDINCGESVEKLARDARYQAFQQHIRQGDVLVTGQHIDDQLETFLLALKRGSGPKGLSSMAKVMSFGEAFIVR
PLLSVTRLDIEASAHDMGLTWVEDESNQDLRFDRNFIRHQVTPTLTERWPSFRESVCRSAQLCAEQESLLDELLESHLQQ
ALGGCNSKSNSKSNSKSQSLSIDSLSQHSDLLRARLIRMWLSHCNQPMPSQKQLKLIWDEVACAQADANPKLVLNDVEIR
RFNNQLYLVQDTKDLSSWKSEILIDENLLLPDGLGEIHLKAVSSGSASHNRDVQRFSLTKANGTLRVIFNPEGVSAHPVG
RGHSRKLKKLFQEYQVPSWLRRRTPILMDGDRVIAVLGLFVDKNYEGQDCEALWSK

Sequences:

>Translated_456_residues
MTHLIETFTSVLHQSALKPSRLIVAFSGGVDSRVLLELAAQYAQTHGIECCAVHVHHGLSKNADLWAEQCQTWCDALSVS
LAVERVSLDINCGESVEKLARDARYQAFQQHIRQGDVLVTGQHIDDQLETFLLALKRGSGPKGLSSMAKVMSFGEAFIVR
PLLSVTRLDIEASAHDMGLTWVEDESNQDLRFDRNFIRHQVTPTLTERWPSFRESVCRSAQLCAEQESLLDELLESHLQQ
ALGGCNSKSNSKSNSKSQSLSIDSLSQHSDLLRARLIRMWLSHCNQPMPSQKQLKLIWDEVACAQADANPKLVLNDVEIR
RFNNQLYLVQDTKDLSSWKSEILIDENLLLPDGLGEIHLKAVSSGSASHNRDVQRFSLTKANGTLRVIFNPEGVSAHPVG
RGHSRKLKKLFQEYQVPSWLRRRTPILMDGDRVIAVLGLFVDKNYEGQDCEALWSK
>Mature_455_residues
THLIETFTSVLHQSALKPSRLIVAFSGGVDSRVLLELAAQYAQTHGIECCAVHVHHGLSKNADLWAEQCQTWCDALSVSL
AVERVSLDINCGESVEKLARDARYQAFQQHIRQGDVLVTGQHIDDQLETFLLALKRGSGPKGLSSMAKVMSFGEAFIVRP
LLSVTRLDIEASAHDMGLTWVEDESNQDLRFDRNFIRHQVTPTLTERWPSFRESVCRSAQLCAEQESLLDELLESHLQQA
LGGCNSKSNSKSNSKSQSLSIDSLSQHSDLLRARLIRMWLSHCNQPMPSQKQLKLIWDEVACAQADANPKLVLNDVEIRR
FNNQLYLVQDTKDLSSWKSEILIDENLLLPDGLGEIHLKAVSSGSASHNRDVQRFSLTKANGTLRVIFNPEGVSAHPVGR
GHSRKLKKLFQEYQVPSWLRRRTPILMDGDRVIAVLGLFVDKNYEGQDCEALWSK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=457, Percent_Identity=43.9824945295405, Blast_Score=322, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_VIBSL (B7VIQ1)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417922.1
- GeneID:   7161876
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2335
- HOGENOM:   HBG668702
- OMA:   WWIKSVA
- ProtClustDB:   CLSK874732
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 51170; Mature: 51039

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLIETFTSVLHQSALKPSRLIVAFSGGVDSRVLLELAAQYAQTHGIECCAVHVHHGLS
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCC
KNADLWAEQCQTWCDALSVSLAVERVSLDINCGESVEKLARDARYQAFQQHIRQGDVLVT
CCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
GQHIDDQLETFLLALKRGSGPKGLSSMAKVMSFGEAFIVRPLLSVTRLDIEASAHDMGLT
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
WVEDESNQDLRFDRNFIRHQVTPTLTERWPSFRESVCRSAQLCAEQESLLDELLESHLQQ
EEECCCCCCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGGCNSKSNSKSNSKSQSLSIDSLSQHSDLLRARLIRMWLSHCNQPMPSQKQLKLIWDE
HHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VACAQADANPKLVLNDVEIRRFNNQLYLVQDTKDLSSWKSEILIDENLLLPDGLGEIHLK
HHHHCCCCCCCEEEECEEEEEECCEEEEEECCHHHHHHHHHEEECCCEECCCCCCCEEEE
AVSSGSASHNRDVQRFSLTKANGTLRVIFNPEGVSAHPVGRGHSRKLKKLFQEYQVPSWL
EECCCCCCCCCCHHHEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
RRRTPILMDGDRVIAVLGLFVDKNYEGQDCEALWSK
HHCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHCCC
>Mature Secondary Structure 
THLIETFTSVLHQSALKPSRLIVAFSGGVDSRVLLELAAQYAQTHGIECCAVHVHHGLS
CHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCC
KNADLWAEQCQTWCDALSVSLAVERVSLDINCGESVEKLARDARYQAFQQHIRQGDVLVT
CCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
GQHIDDQLETFLLALKRGSGPKGLSSMAKVMSFGEAFIVRPLLSVTRLDIEASAHDMGLT
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
WVEDESNQDLRFDRNFIRHQVTPTLTERWPSFRESVCRSAQLCAEQESLLDELLESHLQQ
EEECCCCCCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGGCNSKSNSKSNSKSQSLSIDSLSQHSDLLRARLIRMWLSHCNQPMPSQKQLKLIWDE
HHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VACAQADANPKLVLNDVEIRRFNNQLYLVQDTKDLSSWKSEILIDENLLLPDGLGEIHLK
HHHHCCCCCCCEEEECEEEEEECCEEEEEECCHHHHHHHHHEEECCCEECCCCCCCEEEE
AVSSGSASHNRDVQRFSLTKANGTLRVIFNPEGVSAHPVGRGHSRKLKKLFQEYQVPSWL
EECCCCCCCCCCHHHEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
RRRTPILMDGDRVIAVLGLFVDKNYEGQDCEALWSK
HHCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA