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The map label for this gene is glnB [H]

Identifier: 218710299

GI number: 218710299

Start: 2500571

End: 2500915

Strand: Reverse

Name: glnB [H]

Synonym: VS_2333

Alternate gene names: 218710299

Gene position: 2500915-2500571 (Counterclockwise)

Preceding gene: 218710300

Following gene: 218710298

Centisome position: 75.8

GC content: 41.45

Gene sequence:

>345_bases
ATGAACATGAAAAAGATTGAAGCCATTATCAAGCCATTCAAACTTGATGATGTACGTGAAGCACTTGCAGAAGTGGGTAT
TACGGGTATGACAGTATCTGAAGTTAAAGGCTTTGGACGTCAAAAAGGTCATACTGAGCTATACCGTGGCGCAGAGTACA
TGGTCGATTTTTTACCTAAAGTGAAATTGGAAATTGTTGTGACCGACGAAGTGGCTGACCAATGTGTTGATACCATTATC
GAAACGGCTCAAACAGGTAAAATTGGTGACGGTAAGATCTTCATTACTGAAGTTGAACGTGTGGTACGTATTCGTACTGG
CGAAGAAGACGAAGACGCCGTATAA

Upstream 100 bases:

>100_bases
CAAGATGATAAATTTCGAGCCAGAGCTTTAATGCTCTGGCTTTTTTCATTGAATGTTTACTATAGCTAACCGATAATGAG
CTATTCGCACAGTTTAAGGG

Downstream 100 bases:

>100_bases
AAAAACTATTACCTTAAAAGGAGCGTTTATCGCTCCTTTTTCATTTCTGGTAGGGTGCTGTATGTTTAAGAAAACCATCA
TTGTTATCACGCTATTGATA

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MNMKKIEAIIKPFKLDDVREALAEVGITGMTVSEVKGFGRQKGHTELYRGAEYMVDFLPKVKLEIVVTDEVADQCVDTII
ETAQTGKIGDGKIFITEVERVVRIRTGEEDEDAV

Sequences:

>Translated_114_residues
MNMKKIEAIIKPFKLDDVREALAEVGITGMTVSEVKGFGRQKGHTELYRGAEYMVDFLPKVKLEIVVTDEVADQCVDTII
ETAQTGKIGDGKIFITEVERVVRIRTGEEDEDAV
>Mature_114_residues
MNMKKIEAIIKPFKLDDVREALAEVGITGMTVSEVKGFGRQKGHTELYRGAEYMVDFLPKVKLEIVVTDEVADQCVDTII
ETAQTGKIGDGKIFITEVERVVRIRTGEEDEDAV

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=84.8214285714286, Blast_Score=194, Evalue=1e-51,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=64.2857142857143, Blast_Score=158, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12717; Mature: 12717

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKKIEAIIKPFKLDDVREALAEVGITGMTVSEVKGFGRQKGHTELYRGAEYMVDFLPK
CCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
VKLEIVVTDEVADQCVDTIIETAQTGKIGDGKIFITEVERVVRIRTGEEDEDAV
CEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNMKKIEAIIKPFKLDDVREALAEVGITGMTVSEVKGFGRQKGHTELYRGAEYMVDFLPK
CCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
VKLEIVVTDEVADQCVDTIIETAQTGKIGDGKIFITEVERVVRIRTGEEDEDAV
CEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]