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The map label for this gene is dnaQ [H]

Identifier: 218710290

GI number: 218710290

Start: 2492966

End: 2493733

Strand: Reverse

Name: dnaQ [H]

Synonym: VS_2324

Alternate gene names: 218710290

Gene position: 2493733-2492966 (Counterclockwise)

Preceding gene: 218710292

Following gene: 218710289

Centisome position: 75.58

GC content: 45.83

Gene sequence:

>768_bases
ATGAATACCAGTAGCACTCCAGAACAAAACACGAACGAAAAAAACAGTTCGGACGAAAATAAGCGCATCATTGTACTCGA
TACCGAAACCACCGGTATGAATACAGAAGGTGGCCCTCACTATATGGGGCATCGCATCGTTGAAATTGGTGCGGTAGAGA
TCATCAACCGTAAGCTGACCGGGCGTCACTTTCACGTCTATATCAAGCCCGATCGTGCGATTCAAGAAGAGGCAATTGGC
GTTCACGGTATTACCGATGAATTTTTGATTGATAAACCAGAATACCAAGACATACATAAAGAGTTTCTCGACTTTATTAA
AGGTGCGGAGCTGGTGGCTCATAACGCGCCCTTCGATACGGGCTTTATGGACTATGAGTTTGAGAAACTTAACCCTGCTA
TAGGTAAAACGGATGACTACTGTAAAGTTACCGATACCTTGGCGATGGCGAAGAAGATTTTCCCAGGGAAAAGAAACAAT
CTAGATATCTTATGTGACCGTTATGGTATTGATAACTCACACCGTACTCTTCACGGCGCATTGCTCGATGCGGAGATTCT
AGCTGACGTCTACTTATTGATGACAGGTGGGCAAACTTCGCTGCAATTTAACGCTGGCCAACAAGAAGGCGAAGGCGAAA
GCATACGAAGAGCAGAAAGTGGTCGAAAATCCCTAAAGGTTTTGCGAGCTACGGCCGATGAAGTAGAAGCGCATCAAAGT
CGTTTAGACCTCGTCGAGAAAAGCGGAAGCTGCCTCTGGCGTCAGTAG

Upstream 100 bases:

>100_bases
CCTAAACAAGAACCATCAGTGAAAATTTCAACTTGTTTCGTCATGATTTGATACTATTACCTCAAGCACATTATCGGCAT
AGTCTGACACAGTTATCCTT

Downstream 100 bases:

>100_bases
GGAGAAAGTATGTTAAGGGTATTGGCTACCGGTTGTTTTTTGCTGTTGAGCTTTGGCTTACACGCAGCAACAGAATCTGT
AATGAGTTTATTGGATAATC

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNTSSTPEQNTNEKNSSDENKRIIVLDTETTGMNTEGGPHYMGHRIVEIGAVEIINRKLTGRHFHVYIKPDRAIQEEAIG
VHGITDEFLIDKPEYQDIHKEFLDFIKGAELVAHNAPFDTGFMDYEFEKLNPAIGKTDDYCKVTDTLAMAKKIFPGKRNN
LDILCDRYGIDNSHRTLHGALLDAEILADVYLLMTGGQTSLQFNAGQQEGEGESIRRAESGRKSLKVLRATADEVEAHQS
RLDLVEKSGSCLWRQ

Sequences:

>Translated_255_residues
MNTSSTPEQNTNEKNSSDENKRIIVLDTETTGMNTEGGPHYMGHRIVEIGAVEIINRKLTGRHFHVYIKPDRAIQEEAIG
VHGITDEFLIDKPEYQDIHKEFLDFIKGAELVAHNAPFDTGFMDYEFEKLNPAIGKTDDYCKVTDTLAMAKKIFPGKRNN
LDILCDRYGIDNSHRTLHGALLDAEILADVYLLMTGGQTSLQFNAGQQEGEGESIRRAESGRKSLKVLRATADEVEAHQS
RLDLVEKSGSCLWRQ
>Mature_255_residues
MNTSSTPEQNTNEKNSSDENKRIIVLDTETTGMNTEGGPHYMGHRIVEIGAVEIINRKLTGRHFHVYIKPDRAIQEEAIG
VHGITDEFLIDKPEYQDIHKEFLDFIKGAELVAHNAPFDTGFMDYEFEKLNPAIGKTDDYCKVTDTLAMAKKIFPGKRNN
LDILCDRYGIDNSHRTLHGALLDAEILADVYLLMTGGQTSLQFNAGQQEGEGESIRRAESGRKSLKVLRATADEVEAHQS
RLDLVEKSGSCLWRQ

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=238, Percent_Identity=63.0252100840336, Blast_Score=309, Evalue=1e-85,
Organism=Escherichia coli, GI1788149, Length=188, Percent_Identity=29.7872340425532, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 28636; Mature: 28636

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSSTPEQNTNEKNSSDENKRIIVLDTETTGMNTEGGPHYMGHRIVEIGAVEIINRKLT
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHCCEEEEHHHHHHHHHCCC
GRHFHVYIKPDRAIQEEAIGVHGITDEFLIDKPEYQDIHKEFLDFIKGAELVAHNAPFDT
CCEEEEEECCCHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCCCCC
GFMDYEFEKLNPAIGKTDDYCKVTDTLAMAKKIFPGKRNNLDILCDRYGIDNSHRTLHGA
CCCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHH
LLDAEILADVYLLMTGGQTSLQFNAGQQEGEGESIRRAESGRKSLKVLRATADEVEAHQS
HHHHHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLDLVEKSGSCLWRQ
HHHHHHCCCCEECCC
>Mature Secondary Structure
MNTSSTPEQNTNEKNSSDENKRIIVLDTETTGMNTEGGPHYMGHRIVEIGAVEIINRKLT
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHCCEEEEHHHHHHHHHCCC
GRHFHVYIKPDRAIQEEAIGVHGITDEFLIDKPEYQDIHKEFLDFIKGAELVAHNAPFDT
CCEEEEEECCCHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCCCCC
GFMDYEFEKLNPAIGKTDDYCKVTDTLAMAKKIFPGKRNNLDILCDRYGIDNSHRTLHGA
CCCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHH
LLDAEILADVYLLMTGGQTSLQFNAGQQEGEGESIRRAESGRKSLKVLRATADEVEAHQS
HHHHHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLDLVEKSGSCLWRQ
HHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3023634; 6316347; 3540531; 9278503; 1575709 [H]