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The map label for this gene is bar [H]

Identifier: 218709273

GI number: 218709273

Start: 1377304

End: 1377810

Strand: Direct

Name: bar [H]

Synonym: VS_1280

Alternate gene names: 218709273

Gene position: 1377304-1377810 (Clockwise)

Preceding gene: 218709272

Following gene: 218709274

Centisome position: 41.75

GC content: 41.22

Gene sequence:

>507_bases
ATGGAAATCAGAGTAGGGACATTACCTGATATTGCAGGCATCACCGATATCTTCAACTTTTATATTGAACATACCAATGC
GCGCTTTGAAGAAGAGAAGTTGTCGTTGGAAAATCGCCAGCAGTGGTTTTCTCAATTTTCTAGTCAAAGTAAATATCAAC
TTTATGTCGCGACAGAAGGTGATGCGTTATTAGGCTTCGCGTGTTCTCAGCAATACCGGGTTATGTCGGCATTTGAAGAT
ACCGCAGAGGTCACTATTTATCTTGCGACAGAAGCTCAAGGTAAAGGCTTAGGCTCTAAGCTTTATTCTCAGCTATTTGC
ATCGATTAGTGATTATGGTGTTCACCGCGTACTCTCAGGTGTTGCTTTACCTAATGAGGCTTCAATAGCACTTCATAAAC
GTTTTGGATTTCGAGAAGTCGGAGTGTTCAATGAATATGCGAAGAAAAACGGTCAATACATCAGTTCAATGTGGTTAGAG
AAGGCGTTAACCAAGGAACCAGATTGA

Upstream 100 bases:

>100_bases
GATGGTGATCGTGATGGTATTCCTTGCGAGAGTCAGTTCTGCAGTCACTATTGATGCCTGTTTACTAGTCATTCAAAATA
GACAACTAAAAGGACATTAC

Downstream 100 bases:

>100_bases
TTAGGATGGTTTAAACACGATGCAATACTCTTGGTACTTAAATTACGTATTGCTCAATGTTTGATTTAGAAACAAAGGAC
ACAATATGGAAGTAAGGTTA

Product: putative acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MEIRVGTLPDIAGITDIFNFYIEHTNARFEEEKLSLENRQQWFSQFSSQSKYQLYVATEGDALLGFACSQQYRVMSAFED
TAEVTIYLATEAQGKGLGSKLYSQLFASISDYGVHRVLSGVALPNEASIALHKRFGFREVGVFNEYAKKNGQYISSMWLE
KALTKEPD

Sequences:

>Translated_168_residues
MEIRVGTLPDIAGITDIFNFYIEHTNARFEEEKLSLENRQQWFSQFSSQSKYQLYVATEGDALLGFACSQQYRVMSAFED
TAEVTIYLATEAQGKGLGSKLYSQLFASISDYGVHRVLSGVALPNEASIALHKRFGFREVGVFNEYAKKNGQYISSMWLE
KALTKEPD
>Mature_168_residues
MEIRVGTLPDIAGITDIFNFYIEHTNARFEEEKLSLENRQQWFSQFSSQSKYQLYVATEGDALLGFACSQQYRVMSAFED
TAEVTIYLATEAQGKGLGSKLYSQLFASISDYGVHRVLSGVALPNEASIALHKRFGFREVGVFNEYAKKNGQYISSMWLE
KALTKEPD

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=170, Percent_Identity=30, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 18992; Mature: 18992

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRVGTLPDIAGITDIFNFYIEHTNARFEEEKLSLENRQQWFSQFSSQSKYQLYVATEG
CEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
DALLGFACSQQYRVMSAFEDTAEVTIYLATEAQGKGLGSKLYSQLFASISDYGVHRVLSG
CEEEEHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VALPNEASIALHKRFGFREVGVFNEYAKKNGQYISSMWLEKALTKEPD
CCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEIRVGTLPDIAGITDIFNFYIEHTNARFEEEKLSLENRQQWFSQFSSQSKYQLYVATEG
CEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
DALLGFACSQQYRVMSAFEDTAEVTIYLATEAQGKGLGSKLYSQLFASISDYGVHRVLSG
CEEEEHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VALPNEASIALHKRFGFREVGVFNEYAKKNGQYISSMWLEKALTKEPD
CCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916276; 12000953; 2160942 [H]