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The map label for this gene is garR [H]

Identifier: 218709241

GI number: 218709241

Start: 1343232

End: 1344113

Strand: Direct

Name: garR [H]

Synonym: VS_1248

Alternate gene names: 218709241

Gene position: 1343232-1344113 (Clockwise)

Preceding gene: 218709240

Following gene: 218709244

Centisome position: 40.71

GC content: 47.62

Gene sequence:

>882_bases
ATGACTAAACCTGTAATCGGTTTCATTGGCCTAGGTCTTATGGGCGGCAACATGGTTGAAAACCTGCAAACACGCGGTTA
CCACGTAAACGTAATGGATCTAAGCGAAGAAGCGATGGCTCGTGTTACTGCTCGTGGCAATGCTACAGCTTTCACTTCCG
CTAAAGAGCTTGCAGCTGCAAGTGATATTGTTCAGTTTTGCCTTACCACCTCTGCTGTTGTTGAAAAAATCGTTTACGGC
GAAGACGGCGTTCTAGCGGGCATCAAAGAAGGTGCAGTACTCGTAGACTTCGGTACGTCTATCCCTGCTTCTACTAAGCA
AATCGGCGCAGCTCTTGCTGAAAAAGGCGCGGGCATGATCGACGCACCTCTAGGTCGTACTCCAGCCCATGCTAAAGATG
GTCTTCTGAACATCATGGCTGCTGGCGACATGGAAACTTTCAACAAAGTTAAACCTGTTCTTGAAGAGCAAGGCGAAAAC
GTATTCCACCTTGGCGCTCTAGGTGCTGGTCACGTGACTAAGCTTGTAAACAACTTCATGGGTATGACAACGGTTGCGAC
TATGTCTCAAGCTTTCGCTGTCGCTCAACGCGCTGGTGTTGATGGTCAGCAATTGTTTGACATTATGTCTGCAGGTCCAT
CTAACTCTCCGTTCATGCAATTCTGTAAATTCTACGCGGTAGACGGCGAAGAGAAGCTAGGTTTCTCTGTTGCTAACGCA
AACAAAGATCTTGGTTACTTCCTTGCTCTTTGCGAAGAGCTAGGTACTGAGTCTCTAATCGCTCAAGGTACCGCAACAAG
CCTACAAGCTGCTGTAGATGCTGGCCTTGGTCAAAACGACGTACCAGTAATCTTCGACTACTTCGCTAAACTAGAGAAGT
AA

Upstream 100 bases:

>100_bases
GCTGGATCACGCACGAAAGTCCTAAAACGGTTGCCTTAGGGGATTCGGCTAGTTATTATAATGAGCATATAATCATACAA
TAACATATTTGGAGTTGAAC

Downstream 100 bases:

>100_bases
TCACGTCGTTTAGACACGGTTGGATAAAGAACCTGCTCATTTGAGTGGGTTTTTTTATGCTTGTTTTTAGACATTCTTGG
AACGATATTTTTTAAGGAAG

Product: 2-hydroxy-3-oxopropionate reductase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTKPVIGFIGLGLMGGNMVENLQTRGYHVNVMDLSEEAMARVTARGNATAFTSAKELAAASDIVQFCLTTSAVVEKIVYG
EDGVLAGIKEGAVLVDFGTSIPASTKQIGAALAEKGAGMIDAPLGRTPAHAKDGLLNIMAAGDMETFNKVKPVLEEQGEN
VFHLGALGAGHVTKLVNNFMGMTTVATMSQAFAVAQRAGVDGQQLFDIMSAGPSNSPFMQFCKFYAVDGEEKLGFSVANA
NKDLGYFLALCEELGTESLIAQGTATSLQAAVDAGLGQNDVPVIFDYFAKLEK

Sequences:

>Translated_293_residues
MTKPVIGFIGLGLMGGNMVENLQTRGYHVNVMDLSEEAMARVTARGNATAFTSAKELAAASDIVQFCLTTSAVVEKIVYG
EDGVLAGIKEGAVLVDFGTSIPASTKQIGAALAEKGAGMIDAPLGRTPAHAKDGLLNIMAAGDMETFNKVKPVLEEQGEN
VFHLGALGAGHVTKLVNNFMGMTTVATMSQAFAVAQRAGVDGQQLFDIMSAGPSNSPFMQFCKFYAVDGEEKLGFSVANA
NKDLGYFLALCEELGTESLIAQGTATSLQAAVDAGLGQNDVPVIFDYFAKLEK
>Mature_292_residues
TKPVIGFIGLGLMGGNMVENLQTRGYHVNVMDLSEEAMARVTARGNATAFTSAKELAAASDIVQFCLTTSAVVEKIVYGE
DGVLAGIKEGAVLVDFGTSIPASTKQIGAALAEKGAGMIDAPLGRTPAHAKDGLLNIMAAGDMETFNKVKPVLEEQGENV
FHLGALGAGHVTKLVNNFMGMTTVATMSQAFAVAQRAGVDGQQLFDIMSAGPSNSPFMQFCKFYAVDGEEKLGFSVANAN
KDLGYFLALCEELGTESLIAQGTATSLQAAVDAGLGQNDVPVIFDYFAKLEK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=27.4305555555556, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI23308751, Length=213, Percent_Identity=30.5164319248826, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI145693186, Length=288, Percent_Identity=34.375, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1790315, Length=282, Percent_Identity=28.0141843971631, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1786719, Length=243, Percent_Identity=28.8065843621399, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789092, Length=287, Percent_Identity=26.1324041811847, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788341, Length=211, Percent_Identity=26.0663507109005, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17557316, Length=205, Percent_Identity=30.2439024390244, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24655230, Length=294, Percent_Identity=31.6326530612245, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922568, Length=294, Percent_Identity=31.6326530612245, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=22.6950354609929, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.60 [H]

Molecular weight: Translated: 30645; Mature: 30514

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPVIGFIGLGLMGGNMVENLQTRGYHVNVMDLSEEAMARVTARGNATAFTSAKELAAA
CCCCHHHHHHHHHCCCHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHH
SDIVQFCLTTSAVVEKIVYGEDGVLAGIKEGAVLVDFGTSIPASTKQIGAALAEKGAGMI
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCE
DAPLGRTPAHAKDGLLNIMAAGDMETFNKVKPVLEEQGENVFHLGALGAGHVTKLVNNFM
ECCCCCCCCCHHCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
GMTTVATMSQAFAVAQRAGVDGQQLFDIMSAGPSNSPFMQFCKFYAVDGEEKLGFSVANA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHCEEEECC
NKDLGYFLALCEELGTESLIAQGTATSLQAAVDAGLGQNDVPVIFDYFAKLEK
CCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TKPVIGFIGLGLMGGNMVENLQTRGYHVNVMDLSEEAMARVTARGNATAFTSAKELAAA
CCCHHHHHHHHHCCCHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHH
SDIVQFCLTTSAVVEKIVYGEDGVLAGIKEGAVLVDFGTSIPASTKQIGAALAEKGAGMI
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCE
DAPLGRTPAHAKDGLLNIMAAGDMETFNKVKPVLEEQGENVFHLGALGAGHVTKLVNNFM
ECCCCCCCCCHHCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
GMTTVATMSQAFAVAQRAGVDGQQLFDIMSAGPSNSPFMQFCKFYAVDGEEKLGFSVANA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHCEEEECC
NKDLGYFLALCEELGTESLIAQGTATSLQAAVDAGLGQNDVPVIFDYFAKLEK
CCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]