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The map label for this gene is proW [H]

Identifier: 218709173

GI number: 218709173

Start: 1248076

End: 1249170

Strand: Direct

Name: proW [H]

Synonym: VS_1180

Alternate gene names: 218709173

Gene position: 1248076-1249170 (Clockwise)

Preceding gene: 218709172

Following gene: 218709174

Centisome position: 37.83

GC content: 47.12

Gene sequence:

>1095_bases
ATGTCGTCTGAACAAACAAATAATGACCCATGGTCTCAATCAGCTCCAGCAGCTCAGCCATCAAGTGATCCTTGGGGTCA
AGCAGCTGATACCTCACCATCAGCAGATTGGTTAAGCAGCGAAACAGTCGAAACGACACCTTTTGATCCACTTAACCCTT
TTGCAGAAGCAGTTTTGCCCGTCGACACTTGGGTAGAGTCTGGACTGAATTGGTTAGTTGAGCACGGACGTCCATTGTTT
CAAGCAATCCGTGTGCCTATCGATTTCATTCTAAGTTCATTTGAAACGGCGTTGGTGTCGACACCTGCACCATTCATGTT
GATCATTTTGTTTTTAGTTGCATGGCAGTTTTCGAACCTGAAGCTTGGTTTGGCTACAGCCATTTCTTTGACTTTTATTG
GCTTGATTGGCGCTTGGTCTGGAGCGATGACCACGCTTTCACTGGTCATGACATCGGTATTCTTCTGTCTGCTGATCGGC
TTACCCATGGGGATATGGCTCGCCCGCAGCAATACCGCGGCTAAATTCGTACGCCCAATTCTCGACGCCATGCAAACGAC
GCCAGCCTTCGTTTATCTTGTACCCATCGTAATGTTGTTTGGTATCGGTAACGTTCCGGGTGTGGTGGTGACGATCATAT
TCGCACTTCCTCCTGTTGTACGTTTGACTATCTTGGGTATTCAGCAAGTACCGGAAGATCTGATCGAAGCGGGTCACTCA
TTTGGTGCCAGCAAAAAGCAGATGCTTTACCGAATTCAACTACCACTTGCACTGCCAACCATCATGGCGGGCGTGAACCA
AACACTGATGCTGTCGCTATCGATGGTGGTTATCGCATCAATGATCGCGGTAGGTGGATTAGGTCAAATGGTACTGAGAG
GTATTGGCCGACTGGATATGGGATTAGCCGCTGTTGGTGGCCTAGGCATTGTTATTCTTGCAATCCTACTTGACCGCATT
ACCCAAGTTCTCGGGGTAAATGCAGGTAATACAAAATTACGCTGGTATCACATGGGGCCTGTTTCCCTTGTGCTAAAAGC
TTTAAATCGCGGTAAACAATCAGAACTACAACTAAGGAAAAACACCAATGAATAA

Upstream 100 bases:

>100_bases
CGGTGTGCCTTACTCCGTTCCTGTTGTGGATGAGCAAGGTAATTACTTTGGCGTGGTAACTAAGTCACGACTGTTACAAA
CATTGGATAAGGGGTAGATC

Downstream 100 bases:

>100_bases
TTCATGGAAGAGTAAGCTAGCCGTTAGCATCGTTTCGACACTGGCTGTATCAACGAACGTGTGGGCTGCAAGCTTACCGG
GTGAAGGTGTATCAGTTCAG

Product: glycine betaine/L-proline transport system permease ProW

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSSEQTNNDPWSQSAPAAQPSSDPWGQAADTSPSADWLSSETVETTPFDPLNPFAEAVLPVDTWVESGLNWLVEHGRPLF
QAIRVPIDFILSSFETALVSTPAPFMLIILFLVAWQFSNLKLGLATAISLTFIGLIGAWSGAMTTLSLVMTSVFFCLLIG
LPMGIWLARSNTAAKFVRPILDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPEDLIEAGHS
FGASKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRI
TQVLGVNAGNTKLRWYHMGPVSLVLKALNRGKQSELQLRKNTNE

Sequences:

>Translated_364_residues
MSSEQTNNDPWSQSAPAAQPSSDPWGQAADTSPSADWLSSETVETTPFDPLNPFAEAVLPVDTWVESGLNWLVEHGRPLF
QAIRVPIDFILSSFETALVSTPAPFMLIILFLVAWQFSNLKLGLATAISLTFIGLIGAWSGAMTTLSLVMTSVFFCLLIG
LPMGIWLARSNTAAKFVRPILDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPEDLIEAGHS
FGASKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRI
TQVLGVNAGNTKLRWYHMGPVSLVLKALNRGKQSELQLRKNTNE
>Mature_363_residues
SSEQTNNDPWSQSAPAAQPSSDPWGQAADTSPSADWLSSETVETTPFDPLNPFAEAVLPVDTWVESGLNWLVEHGRPLFQ
AIRVPIDFILSSFETALVSTPAPFMLIILFLVAWQFSNLKLGLATAISLTFIGLIGAWSGAMTTLSLVMTSVFFCLLIGL
PMGIWLARSNTAAKFVRPILDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPEDLIEAGHSF
GASKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLAAVGGLGIVILAILLDRIT
QVLGVNAGNTKLRWYHMGPVSLVLKALNRGKQSELQLRKNTNE

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=348, Percent_Identity=62.9310344827586, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=29.2307692307692, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=33.1168831168831, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788449, Length=187, Percent_Identity=28.3422459893048, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 39147; Mature: 39016

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEQTNNDPWSQSAPAAQPSSDPWGQAADTSPSADWLSSETVETTPFDPLNPFAEAVLP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHH
VDTWVESGLNWLVEHGRPLFQAIRVPIDFILSSFETALVSTPAPFMLIILFLVAWQFSNL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
KLGLATAISLTFIGLIGAWSGAMTTLSLVMTSVFFCLLIGLPMGIWLARSNTAAKFVRPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHH
LDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPEDLIEAGHS
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
FGASKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDM
CCCHHHHHHHHEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHH
GLAAVGGLGIVILAILLDRITQVLGVNAGNTKLRWYHMGPVSLVLKALNRGKQSELQLRK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHC
NTNE
CCCC
>Mature Secondary Structure 
SSEQTNNDPWSQSAPAAQPSSDPWGQAADTSPSADWLSSETVETTPFDPLNPFAEAVLP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHH
VDTWVESGLNWLVEHGRPLFQAIRVPIDFILSSFETALVSTPAPFMLIILFLVAWQFSNL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
KLGLATAISLTFIGLIGAWSGAMTTLSLVMTSVFFCLLIGLPMGIWLARSNTAAKFVRPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHH
LDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPVVRLTILGIQQVPEDLIEAGHS
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
FGASKKQMLYRIQLPLALPTIMAGVNQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDM
CCCHHHHHHHHEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHH
GLAAVGGLGIVILAILLDRITQVLGVNAGNTKLRWYHMGPVSLVLKALNRGKQSELQLRK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHC
NTNE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]