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The map label for this gene is gltX [H]

Identifier: 218708771

GI number: 218708771

Start: 792752

End: 794179

Strand: Direct

Name: gltX [H]

Synonym: VS_0769

Alternate gene names: 218708771

Gene position: 792752-794179 (Clockwise)

Preceding gene: 218708768

Following gene: 218708789

Centisome position: 24.03

GC content: 45.87

Gene sequence:

>1428_bases
ATGACGGTTAAAACTCGTTTTGCTCCTAGCCCAACTGGCTATCTTCACGTTGGTGGTGCACGTACTGCACTTTACTCTTG
GCTATTCGCTAAGAATCAAGGTGGTGAATTCGTTCTGCGTATCGAAGACACGGACCTTGAGCGTAACTCTCAAGAAGCGG
TTGATGCAATTCTAGAAGGCATGCAATGGATGGGTATGGAATGGGATGAAGGTCCTTACTACCAATCTAAGCGTTTTGAC
CGTTACAACGAAATGGTTGATAAGCTACTTGCTGAAGACAAAGCTTTCAAATGCTATGCGTCTAAAGAGCTGCTTGACGA
AATTCGTGCAGAGCAAGAAGAAAACAAAGAGATGGCTCGTTACGATGCGAACCACCCTAAAATTGTTGCAGCAAACGAAG
CAGCAAAAGAAGGTGATGCGTGCGTTATCCGTTTCCGTAACCCTAAAGAAGGCAGCGTAGTCTTTGATGACCAAATTCGT
GGTCGCATTGAAATTTCTAACAGCCAACTTGATGACCTAATCATTCGTCGTACAGACGGTGCACCAACATACAACTTCGT
TGTTGTAGTGGATGACTGGGACATGGGTATTACACACGTTGTACGTGGTGAAGACCACATCAACAACACACCTCGTCAAA
TCAACATCTATGAAGCACTAGGCGCGCCAGTTCCAACTTTCGCTCACTGTGCAATGATTCTTGGTGATGACGGTGCGAAA
CTTTCTAAGCGTCACGGTGCTGTTTCTGTAATGCAATACCGCGACGAAGGTTACCTACCAAATGCACTAAACAACTACCT
AGTTCGTTTAGGTTGGTCTCACGGTGACCAAGAGATCTTCTCTCAAGAAGAGATGATTGAATTCTTCAGCCTGAACGCTA
TCAGCAAGTCTGCTTCTGCATTCAACACTGATAAGCTACTTTGGTTGAACAACCACTACATCAAGACTTCTGAGCCTGAG
TACGTTGCTAAATACCTGCAATGGCACCTAGATGCACAAAAGATCGATACAACAAATGGCCCGGCTATCACTGAAGTGAT
CACGCTAGTTGGCGAGCGTTGTAATACGCTTATCGAACTTGCTGAACAGTCTCGTTACTTCTACGAAGATTTCTCTGAGT
TTGAAGCTGGTGCAGCGAAGAAGCACCTACGTGGTGTTGCTAAAGGACCACTAGAGCTTGCTCTTGCTAAGGTTGAAGCG
CTTGAGGATTTCACTACTGCAAACATCAAAGATGGTGTGATTGCAGCAGTATGTGAAGAGCTAGAGGTAGGCATGGGTAA
AATCGGTATGCCACTTCGCGTAGCAGTAACAGGTGGCGGCCAGTCTCCTTCTGTTGATGCAGTGATGGAGCTTGTTGGTA
AAGAGCGCGTAATCGCTCGCATCAAGATGGCTCTTGAGTTCATCGCTGAGCGTGAAGCTAATGCTTAA

Upstream 100 bases:

>100_bases
GTGGTGATAAAATAGCGCCCAAAATTTAGTGATATTTTTCTGTTGCAGGCTTTGTAATGGCAATCACTAAAAGAATCACC
TACATAATCAAGGTATTTAA

Downstream 100 bases:

>100_bases
TTGAGCTTTTGCTAGATTCAAAAAAGGGTCAGTAACTTGGTTACTGACCCTTTTTATTTGTCTCGAGTTTTAGCTAATAA
ATAGGCTTAAATCTTTTTGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAILEGMQWMGMEWDEGPYYQSKRFD
RYNEMVDKLLAEDKAFKCYASKELLDEIRAEQEENKEMARYDANHPKIVAANEAAKEGDACVIRFRNPKEGSVVFDDQIR
GRIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITHVVRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAK
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMIEFFSLNAISKSASAFNTDKLLWLNNHYIKTSEPE
YVAKYLQWHLDAQKIDTTNGPAITEVITLVGERCNTLIELAEQSRYFYEDFSEFEAGAAKKHLRGVAKGPLELALAKVEA
LEDFTTANIKDGVIAAVCEELEVGMGKIGMPLRVAVTGGGQSPSVDAVMELVGKERVIARIKMALEFIAEREANA

Sequences:

>Translated_475_residues
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAILEGMQWMGMEWDEGPYYQSKRFD
RYNEMVDKLLAEDKAFKCYASKELLDEIRAEQEENKEMARYDANHPKIVAANEAAKEGDACVIRFRNPKEGSVVFDDQIR
GRIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITHVVRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAK
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMIEFFSLNAISKSASAFNTDKLLWLNNHYIKTSEPE
YVAKYLQWHLDAQKIDTTNGPAITEVITLVGERCNTLIELAEQSRYFYEDFSEFEAGAAKKHLRGVAKGPLELALAKVEA
LEDFTTANIKDGVIAAVCEELEVGMGKIGMPLRVAVTGGGQSPSVDAVMELVGKERVIARIKMALEFIAEREANA
>Mature_474_residues
TVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAILEGMQWMGMEWDEGPYYQSKRFDR
YNEMVDKLLAEDKAFKCYASKELLDEIRAEQEENKEMARYDANHPKIVAANEAAKEGDACVIRFRNPKEGSVVFDDQIRG
RIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITHVVRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAKL
SKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMIEFFSLNAISKSASAFNTDKLLWLNNHYIKTSEPEY
VAKYLQWHLDAQKIDTTNGPAITEVITLVGERCNTLIELAEQSRYFYEDFSEFEAGAAKKHLRGVAKGPLELALAKVEAL
EDFTTANIKDGVIAAVCEELEVGMGKIGMPLRVAVTGGGQSPSVDAVMELVGKERVIARIKMALEFIAEREANA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=492, Percent_Identity=30.6910569105691, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI62241042, Length=335, Percent_Identity=24.7761194029851, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788743, Length=472, Percent_Identity=67.7966101694915, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=35.6275303643725, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786895, Length=204, Percent_Identity=26.4705882352941, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=489, Percent_Identity=29.038854805726, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17511007, Length=329, Percent_Identity=25.2279635258359, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=494, Percent_Identity=30.7692307692308, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6321192, Length=215, Percent_Identity=28.8372093023256, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=214, Percent_Identity=26.1682242990654, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=30.9090909090909, Blast_Score=231, Evalue=9e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53271; Mature: 53140

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHHHHHHH
MQWMGMEWDEGPYYQSKRFDRYNEMVDKLLAEDKAFKCYASKELLDEIRAEQEENKEMAR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHH
YDANHPKIVAANEAAKEGDACVIRFRNPKEGSVVFDDQIRGRIEISNSQLDDLIIRRTDG
HCCCCCEEEECCCCCCCCCEEEEEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEECCC
APTYNFVVVVDDWDMGITHVVRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAK
CCCEEEEEEEECCCCCCEEEECCCHHCCCCCCEEEEHHHHCCCCCHHHHEEEEECCCCCH
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMIEFFSLNAISKSASA
HHHHCCCEEEEEECCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
FNTDKLLWLNNHYIKTSEPEYVAKYLQWHLDAQKIDTTNGPAITEVITLVGERCNTLIEL
CCCCEEEEEECCEECCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AEQSRYFYEDFSEFEAGAAKKHLRGVAKGPLELALAKVEALEDFTTANIKDGVIAAVCEE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LEVGMGKIGMPLRVAVTGGGQSPSVDAVMELVGKERVIARIKMALEFIAEREANA
HHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAILEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHHHHHHH
MQWMGMEWDEGPYYQSKRFDRYNEMVDKLLAEDKAFKCYASKELLDEIRAEQEENKEMAR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHH
YDANHPKIVAANEAAKEGDACVIRFRNPKEGSVVFDDQIRGRIEISNSQLDDLIIRRTDG
HCCCCCEEEECCCCCCCCCEEEEEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEECCC
APTYNFVVVVDDWDMGITHVVRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAK
CCCEEEEEEEECCCCCCEEEECCCHHCCCCCCEEEEHHHHCCCCCHHHHEEEEECCCCCH
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMIEFFSLNAISKSASA
HHHHCCCEEEEEECCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
FNTDKLLWLNNHYIKTSEPEYVAKYLQWHLDAQKIDTTNGPAITEVITLVGERCNTLIEL
CCCCEEEEEECCEECCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AEQSRYFYEDFSEFEAGAAKKHLRGVAKGPLELALAKVEALEDFTTANIKDGVIAAVCEE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LEVGMGKIGMPLRVAVTGGGQSPSVDAVMELVGKERVIARIKMALEFIAEREANA
HHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA