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The map label for this gene is ubiF [H]

Identifier: 218708746

GI number: 218708746

Start: 762006

End: 763181

Strand: Direct

Name: ubiF [H]

Synonym: VS_0725

Alternate gene names: 218708746

Gene position: 762006-763181 (Clockwise)

Preceding gene: 218708740

Following gene: 218708754

Centisome position: 23.1

GC content: 44.9

Gene sequence:

>1176_bases
ATGAACAGTTACGATATTGTAGTCGTCGGTGGCGGCATGGTTGGTGCAGCAACAGCAATCGGTTTTGCCAAGCAAGGTCT
GAGTGTTGCGGTGATCGAAGGTTTTGCCCCACAAGCTTTTGATGAGTCGCAAGCGATGGACATTCGTGTGTCAGCGATTT
CTCAAGCATCGGTCGATTTGCTTGAAGATCTTGGTGCATGGCAAAGTATTGCAGCAATGCGAGTGTGTTCTTATAAACGT
TTAGAGACGTGGGAGCACCCAGAGTGTCGTACTCGGTTTGATGCTGGATCACTGGACCTTCCTCGTTTGGGCTATATCGT
TGAGAACAGACTCATTCAATTAGGTTTATGGTCTCAGTTTGATGCCTACAACAATCTCGAACTGTTATGCCCAGAAAAGC
TCGATAAGATTGAGTTTGGTGAAACCAATATCGTAAAGCTTCAATCAGGGGCTGAGTTATCTGCGAAGTGGGTGGTTGGT
GCTGATGGTGCGAATTCAGCCGTTCGACAACAAGCTGGAATTGGCATCACGGCTTGGGATTATCGACAACACTGTATGCT
TATCAATGTAGAAACAGAACAACCTCAGCAAGATATTACTTGGCAGCAATTTCTACCAAGTGGCCCTCGTTCGTTTCTGC
CTTTGTGTTCTCTAACCTCTGACGATCAACAAGTCGGGCAGGGCTCGTTAGTTTGGTATGATTCTCCGCTGCGTATTAAG
CAACTGTCAGCAATGACCCCTGAAAAACTACGTAACGAAGTACTTACTCACTTTCCTAAAGAGTTAGGTGATATCAAAGT
CTTGAATTCGGGTTCTTTTCCTCTGACACGACGTCATGCTCAATCATACTCGAAGAATAACTGTATTTTAGTCGGGGACT
CTGCGCATACGATTAACCCTTTGGCTGGACAGGGGGTTAACTTAGGCTTCAAAGATGTATCAGTACTGTTGAATGTCACC
AAAGAGAAAGGTGAATTGAATCAATCTGTGGCGAGACGCTATGAAGTGATCAGAAGAGGTGACAATCTAATGATGCAAAG
TGGCATGGACTTTTTCTACAAAACGTTTAGCAATGACATTGGCCCACTTAAGTTTGTGCGAAATGCAGCACTAAAGCTAG
CAGAAAACTCAGGGCCGATTAAATCACAGGTGCTTAAGTACGCTTTAGGGTTGTGA

Upstream 100 bases:

>100_bases
AAGGGATAACCCTTTTTTCGAATAAGGTTTTTGGTTACAAGTTCGTTATGAAAAAGTACAATCAAAAACAGTCAAAGAAT
CAAACTGATAAGTACAAAAT

Downstream 100 bases:

>100_bases
TTGGTTACTCCAAGTTATCTAGTTATAAAAGAAGGAGAGCTGAGGCTCTCCTTTTTTGATTTCATGATAGTGCTTAGTGC
TATTGCGTCACACAAACAGG

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNSYDIVVVGGGMVGAATAIGFAKQGLSVAVIEGFAPQAFDESQAMDIRVSAISQASVDLLEDLGAWQSIAAMRVCSYKR
LETWEHPECRTRFDAGSLDLPRLGYIVENRLIQLGLWSQFDAYNNLELLCPEKLDKIEFGETNIVKLQSGAELSAKWVVG
ADGANSAVRQQAGIGITAWDYRQHCMLINVETEQPQQDITWQQFLPSGPRSFLPLCSLTSDDQQVGQGSLVWYDSPLRIK
QLSAMTPEKLRNEVLTHFPKELGDIKVLNSGSFPLTRRHAQSYSKNNCILVGDSAHTINPLAGQGVNLGFKDVSVLLNVT
KEKGELNQSVARRYEVIRRGDNLMMQSGMDFFYKTFSNDIGPLKFVRNAALKLAENSGPIKSQVLKYALGL

Sequences:

>Translated_391_residues
MNSYDIVVVGGGMVGAATAIGFAKQGLSVAVIEGFAPQAFDESQAMDIRVSAISQASVDLLEDLGAWQSIAAMRVCSYKR
LETWEHPECRTRFDAGSLDLPRLGYIVENRLIQLGLWSQFDAYNNLELLCPEKLDKIEFGETNIVKLQSGAELSAKWVVG
ADGANSAVRQQAGIGITAWDYRQHCMLINVETEQPQQDITWQQFLPSGPRSFLPLCSLTSDDQQVGQGSLVWYDSPLRIK
QLSAMTPEKLRNEVLTHFPKELGDIKVLNSGSFPLTRRHAQSYSKNNCILVGDSAHTINPLAGQGVNLGFKDVSVLLNVT
KEKGELNQSVARRYEVIRRGDNLMMQSGMDFFYKTFSNDIGPLKFVRNAALKLAENSGPIKSQVLKYALGL
>Mature_391_residues
MNSYDIVVVGGGMVGAATAIGFAKQGLSVAVIEGFAPQAFDESQAMDIRVSAISQASVDLLEDLGAWQSIAAMRVCSYKR
LETWEHPECRTRFDAGSLDLPRLGYIVENRLIQLGLWSQFDAYNNLELLCPEKLDKIEFGETNIVKLQSGAELSAKWVVG
ADGANSAVRQQAGIGITAWDYRQHCMLINVETEQPQQDITWQQFLPSGPRSFLPLCSLTSDDQQVGQGSLVWYDSPLRIK
QLSAMTPEKLRNEVLTHFPKELGDIKVLNSGSFPLTRRHAQSYSKNNCILVGDSAHTINPLAGQGVNLGFKDVSVLLNVT
KEKGELNQSVARRYEVIRRGDNLMMQSGMDFFYKTFSNDIGPLKFVRNAALKLAENSGPIKSQVLKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=397, Percent_Identity=29.4710327455919, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI32967307, Length=351, Percent_Identity=28.4900284900285, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1786883, Length=393, Percent_Identity=49.8727735368957, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1789273, Length=399, Percent_Identity=36.8421052631579, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789274, Length=399, Percent_Identity=28.5714285714286, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25146631, Length=441, Percent_Identity=26.984126984127, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321694, Length=464, Percent_Identity=23.4913793103448, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19920760, Length=438, Percent_Identity=27.6255707762557, Blast_Score=151, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 43168; Mature: 43168

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSYDIVVVGGGMVGAATAIGFAKQGLSVAVIEGFAPQAFDESQAMDIRVSAISQASVDL
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHH
LEDLGAWQSIAAMRVCSYKRLETWEHPECRTRFDAGSLDLPRLGYIVENRLIQLGLWSQF
HHHHCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHCCEEEEECCHHC
DAYNNLELLCPEKLDKIEFGETNIVKLQSGAELSAKWVVGADGANSAVRQQAGIGITAWD
CCCCCCEEECCCCCCCEECCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHCCCCEEEEC
YRQHCMLINVETEQPQQDITWQQFLPSGPRSFLPLCSLTSDDQQVGQGSLVWYDSPLRIK
CCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHCCCCCHHHCCCCCEEEECCCCHHH
QLSAMTPEKLRNEVLTHFPKELGDIKVLNSGSFPLTRRHAQSYSKNNCILVGDSAHTINP
HHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCCEEEECCCCCCCCC
LAGQGVNLGFKDVSVLLNVTKEKGELNQSVARRYEVIRRGDNLMMQSGMDFFYKTFSNDI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHCCCC
GPLKFVRNAALKLAENSGPIKSQVLKYALGL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNSYDIVVVGGGMVGAATAIGFAKQGLSVAVIEGFAPQAFDESQAMDIRVSAISQASVDL
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHH
LEDLGAWQSIAAMRVCSYKRLETWEHPECRTRFDAGSLDLPRLGYIVENRLIQLGLWSQF
HHHHCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHCCEEEEECCHHC
DAYNNLELLCPEKLDKIEFGETNIVKLQSGAELSAKWVVGADGANSAVRQQAGIGITAWD
CCCCCCEEECCCCCCCEECCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHCCCCEEEEC
YRQHCMLINVETEQPQQDITWQQFLPSGPRSFLPLCSLTSDDQQVGQGSLVWYDSPLRIK
CCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHCCCCCHHHCCCCCEEEECCCCHHH
QLSAMTPEKLRNEVLTHFPKELGDIKVLNSGSFPLTRRHAQSYSKNNCILVGDSAHTINP
HHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCCEEEECCCCCCCCC
LAGQGVNLGFKDVSVLLNVTKEKGELNQSVARRYEVIRRGDNLMMQSGMDFFYKTFSNDI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHCCCC
GPLKFVRNAALKLAENSGPIKSQVLKYALGL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]