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The map label for this gene is leuS [H]

Identifier: 218708739

GI number: 218708739

Start: 752576

End: 755152

Strand: Reverse

Name: leuS [H]

Synonym: VS_0718

Alternate gene names: 218708739

Gene position: 755152-752576 (Counterclockwise)

Preceding gene: 218708741

Following gene: 218708738

Centisome position: 22.89

GC content: 46.6

Gene sequence:

>2577_bases
ATGCAAGAACAATATAACCCGCAAGAGATTGAACAAAAGGTTCAACAACACTGGGATGACAGCGAGACTTTCGTTGTAAG
TGAAGACCCAAACAAAGAAAAATTCTACTGTCTTTCTATGTTCCCGTACCCAAGTGGCCGACTGCACATGGGTCACGTGC
GTAACTACACCATCGGTGATGTGGTATCTCGTTTCCAACGTCTACAAGGCAAAAACGTAATGCAGCCAATCGGTTGGGAT
GCATTCGGCCTACCTGCAGAAAACGCAGCTGTAAAAAACAACACAGCGCCTGCTCCGTGGACTTACGAAAACATCGAATA
CATGAAAAACCAGCTTAAACTTTTAGGCTTTGGTTACGACTGGAAACGTGAATTCGCAACATGTACTCCTGAGTACTACC
GTTGGGAACAAGAGTTCTTCACGAAGCTTTACGAACAAGGCCTAGTTTACAAGAAGACATCTTCTGTTAACTGGTGTCCA
AACGACCAAACTGTACTAGCGAACGAGCAAGTAGAAGACGGTTGCTGCTGGCGTTGTGACACTCCGGTAGAACAAAAGAA
GATCCCACAATGGTTCATTAAAATCACTGAGTACGCTCAAGAGCTACTAGACGATCTAGACAACCTTGAAGGTTGGCCTG
AAATGGTTAAGACCATGCAGCGCAACTGGATCGGTCGCTCTGAAGGTGTAGAGCTATCTTTCGCTGTTAACGGTGAAGAA
GCGCCACTAGAAGTGTATACAACTCGTCCAGACACACTAATGGGTGTTTCTTATGTTGGTATCGCTGCAGGTCACCCACT
TGCAGAGAAAGCATCAAAGAACAACCCAGAGCTTGCAGCATTCGTTGAAGAGTGTCGTAACACCAAAGTGGCTGAAGCAG
AACTAGCAACGATGGAAAAGAAAGGTATGGATACTGGCCTAACGGCTATCCACCCTCTTAACGGTCGTGTTGTTCCTGTT
TACGTAGCTAACTTCGTTCTTATGGATTACGGCACAGGTGCTGTAATGGCGGTTCCTGCTCACGATCAACGTGACTACGA
ATTCGCTACTAAGTACGGCATCGATATCATCCCAGTAATCAAGCCAGAAGATGGTTCTGAGCTAGATGTGTCTGAAGCGG
CTTACACTGAGAAAGGTGTACTGTTCGATTCTGGTGAATTCGATGGTCTTGCATTCCAAGAAGCATTCGATGCAATCGCT
GCGAAGCTTGAAGCTGAAGGCAAAGGTAAGAAAACAGTAAACTTCCGTCTACGTGACTGGGGCGTTTCTCGTCAGCGTTA
CTGGGGTGCTCCAATCCCAATGGTAACCACTGAAGACGGTGAAGTTCACCCTGTACCAGCAGACCAACTGCCCGTTATTC
TTCCAGAAGACGTGGTAATGGATGGCGTAACTAGCCCAATCAAGGCGGATAAGTCTTGGGCTGAAACCACATTCAATGGC
GAACCTGCGCTACGTGAAACTGATACGTTCGATACGTTCATGGAATCCTCATGGTACTACGCACGTTACTGTTCACCACA
AGCTGACGACATTCTAGATCCTGAGAAAGCAAACTACTGGTTGCCAGTTGACCAATACGTTGGTGGTATCGAGCACGCAT
GTATGCACCTGCTTTACTCTCGTTTCTTCCATAAGCTTCTACGTGATGCTGGTTACGTGACGTCTGACGAACCGTTCAAG
CAACTACTGTGTCAAGGCATGGTTCTGGCTGACGCGTTCTATCACGAAAACGAAAAAGGCACCAAAGAGTGGATTGCACC
GACTGACGTAACGGTTGAACGTGACGGTAAAGGTCGCATCGAAAAAGCAGTCGACGATCAAGGTCGTGAAGTTGAACACT
CAGGCATGATCAAAATGTCTAAGTCTAAGAACAACGGTATCGACCCACAAGAGATGGTAGACAAGTACGGCGCTGATACT
GTACGTCTATTCATGATGTTTGCATCGCCTGCAGACATGACGCTTGAGTGGCAAGAGTCTGGCGTTGAAGGGGCAAACCG
TTTCCTTAAGCGTGTTTGGAAACTGGTTCACGCTCACTCTTCTAAAGGTGCAGCTGAAACTGTTGATGCTTCTGCTCTAT
CTGGTAACCAGAAAGCACTTCGTCGTGATATCCACAAAACTATCGCGAAAGTAACGGACGATATCGGCCGTCGCCAAACG
TTCAACACAGCAATAGCTGCAATCATGGAGCTGATGAACAAGCTAGCGAAAGCGCCTCAAGAGTCTGTACAAGATCGTGC
AATTCTTGATGAAGCACTAAAAGCAGTTGTACGTATGCTTTACCCTATGACGCCACACATCTCTTACGAAATGTGGATTG
CGCTTGGTGAATCAAACGTAGACTCAGCAACATGGCCAACGTTTGACGAGAAAGCGCTAGTTGAAGACGAGAAGACTATC
GTTGTAATGATCAACGGTAAGCTACGTGCGAAGCTAACGGTTGCAGCTGATGCAACAGAAGAGCAAGTTCGTGAGCTTGG
TCTAAACGATGAGAACGCTAAGAAATTCCTAGATGGCTTAACTATCCGTAAAGTTATCTTCGTACCTGGTAAGCTTCTAA
ACATCGTTGCTAACTAA

Upstream 100 bases:

>100_bases
ATAACTATTGTTGTACTCAGCTCCTTTAGGTATTCTATGACGATCTGTAAGATTCTGTTCTAACCGAACTCACAAATTCC
AAGATAACCGGATACCATCG

Downstream 100 bases:

>100_bases
TTTCGTTAGCACAGTGCAATTACTGAAGAATTAACGAACTTAATTCATCAAAAGCAGTCTGTAGACAGGGTAGCTAATAG
CCCTGTCTACTTATTTAAGC

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 858; Mature: 858

Protein sequence:

>858_residues
MQEQYNPQEIEQKVQQHWDDSETFVVSEDPNKEKFYCLSMFPYPSGRLHMGHVRNYTIGDVVSRFQRLQGKNVMQPIGWD
AFGLPAENAAVKNNTAPAPWTYENIEYMKNQLKLLGFGYDWKREFATCTPEYYRWEQEFFTKLYEQGLVYKKTSSVNWCP
NDQTVLANEQVEDGCCWRCDTPVEQKKIPQWFIKITEYAQELLDDLDNLEGWPEMVKTMQRNWIGRSEGVELSFAVNGEE
APLEVYTTRPDTLMGVSYVGIAAGHPLAEKASKNNPELAAFVEECRNTKVAEAELATMEKKGMDTGLTAIHPLNGRVVPV
YVANFVLMDYGTGAVMAVPAHDQRDYEFATKYGIDIIPVIKPEDGSELDVSEAAYTEKGVLFDSGEFDGLAFQEAFDAIA
AKLEAEGKGKKTVNFRLRDWGVSRQRYWGAPIPMVTTEDGEVHPVPADQLPVILPEDVVMDGVTSPIKADKSWAETTFNG
EPALRETDTFDTFMESSWYYARYCSPQADDILDPEKANYWLPVDQYVGGIEHACMHLLYSRFFHKLLRDAGYVTSDEPFK
QLLCQGMVLADAFYHENEKGTKEWIAPTDVTVERDGKGRIEKAVDDQGREVEHSGMIKMSKSKNNGIDPQEMVDKYGADT
VRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVHAHSSKGAAETVDASALSGNQKALRRDIHKTIAKVTDDIGRRQT
FNTAIAAIMELMNKLAKAPQESVQDRAILDEALKAVVRMLYPMTPHISYEMWIALGESNVDSATWPTFDEKALVEDEKTI
VVMINGKLRAKLTVAADATEEQVRELGLNDENAKKFLDGLTIRKVIFVPGKLLNIVAN

Sequences:

>Translated_858_residues
MQEQYNPQEIEQKVQQHWDDSETFVVSEDPNKEKFYCLSMFPYPSGRLHMGHVRNYTIGDVVSRFQRLQGKNVMQPIGWD
AFGLPAENAAVKNNTAPAPWTYENIEYMKNQLKLLGFGYDWKREFATCTPEYYRWEQEFFTKLYEQGLVYKKTSSVNWCP
NDQTVLANEQVEDGCCWRCDTPVEQKKIPQWFIKITEYAQELLDDLDNLEGWPEMVKTMQRNWIGRSEGVELSFAVNGEE
APLEVYTTRPDTLMGVSYVGIAAGHPLAEKASKNNPELAAFVEECRNTKVAEAELATMEKKGMDTGLTAIHPLNGRVVPV
YVANFVLMDYGTGAVMAVPAHDQRDYEFATKYGIDIIPVIKPEDGSELDVSEAAYTEKGVLFDSGEFDGLAFQEAFDAIA
AKLEAEGKGKKTVNFRLRDWGVSRQRYWGAPIPMVTTEDGEVHPVPADQLPVILPEDVVMDGVTSPIKADKSWAETTFNG
EPALRETDTFDTFMESSWYYARYCSPQADDILDPEKANYWLPVDQYVGGIEHACMHLLYSRFFHKLLRDAGYVTSDEPFK
QLLCQGMVLADAFYHENEKGTKEWIAPTDVTVERDGKGRIEKAVDDQGREVEHSGMIKMSKSKNNGIDPQEMVDKYGADT
VRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVHAHSSKGAAETVDASALSGNQKALRRDIHKTIAKVTDDIGRRQT
FNTAIAAIMELMNKLAKAPQESVQDRAILDEALKAVVRMLYPMTPHISYEMWIALGESNVDSATWPTFDEKALVEDEKTI
VVMINGKLRAKLTVAADATEEQVRELGLNDENAKKFLDGLTIRKVIFVPGKLLNIVAN
>Mature_858_residues
MQEQYNPQEIEQKVQQHWDDSETFVVSEDPNKEKFYCLSMFPYPSGRLHMGHVRNYTIGDVVSRFQRLQGKNVMQPIGWD
AFGLPAENAAVKNNTAPAPWTYENIEYMKNQLKLLGFGYDWKREFATCTPEYYRWEQEFFTKLYEQGLVYKKTSSVNWCP
NDQTVLANEQVEDGCCWRCDTPVEQKKIPQWFIKITEYAQELLDDLDNLEGWPEMVKTMQRNWIGRSEGVELSFAVNGEE
APLEVYTTRPDTLMGVSYVGIAAGHPLAEKASKNNPELAAFVEECRNTKVAEAELATMEKKGMDTGLTAIHPLNGRVVPV
YVANFVLMDYGTGAVMAVPAHDQRDYEFATKYGIDIIPVIKPEDGSELDVSEAAYTEKGVLFDSGEFDGLAFQEAFDAIA
AKLEAEGKGKKTVNFRLRDWGVSRQRYWGAPIPMVTTEDGEVHPVPADQLPVILPEDVVMDGVTSPIKADKSWAETTFNG
EPALRETDTFDTFMESSWYYARYCSPQADDILDPEKANYWLPVDQYVGGIEHACMHLLYSRFFHKLLRDAGYVTSDEPFK
QLLCQGMVLADAFYHENEKGTKEWIAPTDVTVERDGKGRIEKAVDDQGREVEHSGMIKMSKSKNNGIDPQEMVDKYGADT
VRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVHAHSSKGAAETVDASALSGNQKALRRDIHKTIAKVTDDIGRRQT
FNTAIAAIMELMNKLAKAPQESVQDRAILDEALKAVVRMLYPMTPHISYEMWIALGESNVDSATWPTFDEKALVEDEKTI
VVMINGKLRAKLTVAADATEEQVRELGLNDENAKKFLDGLTIRKVIFVPGKLLNIVAN

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=887, Percent_Identity=35.9639233370913, Blast_Score=523, Evalue=1e-148,
Organism=Homo sapiens, GI5454158, Length=389, Percent_Identity=24.9357326478149, Blast_Score=100, Evalue=1e-20,
Organism=Homo sapiens, GI46852147, Length=178, Percent_Identity=26.9662921348315, Blast_Score=71, Evalue=5e-12,
Organism=Escherichia coli, GI1786861, Length=858, Percent_Identity=74.3589743589744, Blast_Score=1358, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=377, Percent_Identity=25.7294429708223, Blast_Score=119, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71997517, Length=813, Percent_Identity=32.4723247232472, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71997510, Length=813, Percent_Identity=32.4723247232472, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI212645227, Length=383, Percent_Identity=30.8093994778068, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71980946, Length=154, Percent_Identity=31.1688311688312, Blast_Score=74, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554638, Length=86, Percent_Identity=38.3720930232558, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=847, Percent_Identity=36.3636363636364, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6321531, Length=386, Percent_Identity=25.3886010362694, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6325217, Length=156, Percent_Identity=28.8461538461538, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21355409, Length=883, Percent_Identity=33.8618346545866, Blast_Score=447, Evalue=1e-125,
Organism=Drosophila melanogaster, GI281366294, Length=163, Percent_Identity=31.9018404907975, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96922; Mature: 96922

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEQYNPQEIEQKVQQHWDDSETFVVSEDPNKEKFYCLSMFPYPSGRLHMGHVRNYTIGD
CCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEECCCCCCCHHH
VVSRFQRLQGKNVMQPIGWDAFGLPAENAAVKNNTAPAPWTYENIEYMKNQLKLLGFGYD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCC
WKREFATCTPEYYRWEQEFFTKLYEQGLVYKKTSSVNWCPNDQTVLANEQVEDGCCWRCD
HHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCCCC
TPVEQKKIPQWFIKITEYAQELLDDLDNLEGWPEMVKTMQRNWIGRSEGVELSFAVNGEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCC
APLEVYTTRPDTLMGVSYVGIAAGHPLAEKASKNNPELAAFVEECRNTKVAEAELATMEK
CCEEEEECCCCCCCCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGMDTGLTAIHPLNGRVVPVYVANFVLMDYGTGAVMAVPAHDQRDYEFATKYGIDIIPVI
CCCCCCCCEEECCCCCEEEEEEHEEEEEECCCCEEEEECCCCCCCCHHHHHCCCEEEEEE
KPEDGSELDVSEAAYTEKGVLFDSGEFDGLAFQEAFDAIAAKLEAEGKGKKTVNFRLRDW
CCCCCCCCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
GVSRQRYWGAPIPMVTTEDGEVHPVPADQLPVILPEDVVMDGVTSPIKADKSWAETTFNG
CCCCHHCCCCCCCEEECCCCCCCCCCCCCCCEECCCHHHHCCCCCCCCCCCCHHHCCCCC
EPALRETDTFDTFMESSWYYARYCSPQADDILDPEKANYWLPVDQYVGGIEHACMHLLYS
CCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEHHHHHCHHHHHHHHHHHH
RFFHKLLRDAGYVTSDEPFKQLLCQGMVLADAFYHENEKGTKEWIAPTDVTVERDGKGRI
HHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCCEEEECCCCCCH
EKAVDDQGREVEHSGMIKMSKSKNNGIDPQEMVDKYGADTVRLFMMFASPADMTLEWQES
HHHHCCCCCEECCCCCEEEECCCCCCCCHHHHHHHHCCHHEEEEHHHCCCCCCEEEEHHC
GVEGANRFLKRVWKLVHAHSSKGAAETVDASALSGNQKALRRDIHKTIAKVTDDIGRRQT
CCHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FNTAIAAIMELMNKLAKAPQESVQDRAILDEALKAVVRMLYPMTPHISYEMWIALGESNV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
DSATWPTFDEKALVEDEKTIVVMINGKLRAKLTVAADATEEQVRELGLNDENAKKFLDGL
CCCCCCCCCCCCEECCCCEEEEEECCCEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHCH
TIRKVIFVPGKLLNIVAN
HHHHHEECCHHHHHHHCC
>Mature Secondary Structure
MQEQYNPQEIEQKVQQHWDDSETFVVSEDPNKEKFYCLSMFPYPSGRLHMGHVRNYTIGD
CCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEECCCCCCCHHH
VVSRFQRLQGKNVMQPIGWDAFGLPAENAAVKNNTAPAPWTYENIEYMKNQLKLLGFGYD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCC
WKREFATCTPEYYRWEQEFFTKLYEQGLVYKKTSSVNWCPNDQTVLANEQVEDGCCWRCD
HHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCCCC
TPVEQKKIPQWFIKITEYAQELLDDLDNLEGWPEMVKTMQRNWIGRSEGVELSFAVNGEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCC
APLEVYTTRPDTLMGVSYVGIAAGHPLAEKASKNNPELAAFVEECRNTKVAEAELATMEK
CCEEEEECCCCCCCCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGMDTGLTAIHPLNGRVVPVYVANFVLMDYGTGAVMAVPAHDQRDYEFATKYGIDIIPVI
CCCCCCCCEEECCCCCEEEEEEHEEEEEECCCCEEEEECCCCCCCCHHHHHCCCEEEEEE
KPEDGSELDVSEAAYTEKGVLFDSGEFDGLAFQEAFDAIAAKLEAEGKGKKTVNFRLRDW
CCCCCCCCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
GVSRQRYWGAPIPMVTTEDGEVHPVPADQLPVILPEDVVMDGVTSPIKADKSWAETTFNG
CCCCHHCCCCCCCEEECCCCCCCCCCCCCCCEECCCHHHHCCCCCCCCCCCCHHHCCCCC
EPALRETDTFDTFMESSWYYARYCSPQADDILDPEKANYWLPVDQYVGGIEHACMHLLYS
CCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEHHHHHCHHHHHHHHHHHH
RFFHKLLRDAGYVTSDEPFKQLLCQGMVLADAFYHENEKGTKEWIAPTDVTVERDGKGRI
HHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCCEEEECCCCCCH
EKAVDDQGREVEHSGMIKMSKSKNNGIDPQEMVDKYGADTVRLFMMFASPADMTLEWQES
HHHHCCCCCEECCCCCEEEECCCCCCCCHHHHHHHHCCHHEEEEHHHCCCCCCEEEEHHC
GVEGANRFLKRVWKLVHAHSSKGAAETVDASALSGNQKALRRDIHKTIAKVTDDIGRRQT
CCHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FNTAIAAIMELMNKLAKAPQESVQDRAILDEALKAVVRMLYPMTPHISYEMWIALGESNV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
DSATWPTFDEKALVEDEKTIVVMINGKLRAKLTVAADATEEQVRELGLNDENAKKFLDGL
CCCCCCCCCCCCEECCCCEEEEEECCCEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHCH
TIRKVIFVPGKLLNIVAN
HHHHHEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA