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The map label for this gene is dinB [H]

Identifier: 218708723

GI number: 218708723

Start: 735949

End: 737025

Strand: Direct

Name: dinB [H]

Synonym: VS_0702

Alternate gene names: 218708723

Gene position: 735949-737025 (Clockwise)

Preceding gene: 218708721

Following gene: 218708725

Centisome position: 22.31

GC content: 45.22

Gene sequence:

>1077_bases
ATGTGTAGTGCGGGTGAAGAGAAAGTAAGAAAGATAATCCACGTTGATATGGACTGTTTTTACGCAGCTGTTGAAATGAG
AGATAATCCTGCTTACCGAAATCGACCACTTGCAGTAGGGGGGCATGAAAAGCAGCGTGGCGTTTTGAGTACCTGTAATT
ACGAAGCTCGCAAGTTTGGTATTCGTTCTGCAATGCCGACGGGAAAGGCGTTGCAGCTTTGTCCCAATCTGTTGGTTGTT
CCTGGGAGGATGTCGGTCTACGTTGAGATATCGAAAAAGATTCGCGAGATTTTTTCTCGATATACATCCATCATTGAACC
TCTGTCTTTAGATGAAGCTTTTCTTGACGTCACGGATTCGACGCTGTGTCACGGTTCTGCGACCCTGATTGCTGAGTCAA
TTCGTCGTGATATTTGGAACGAGTTAAATCTCACGGCTTCAGCGGGTGCCGCTCCGATTAAATTTTTGGCGAAGGTGGCC
TCGGATCTGAATAAGCCCAATGGTCAGTTTGTTATTCCTCCACAGGATGTTCAATCAGTGATTGATGAGCTGCCACTCGA
AAAAATCCCAGGCGTTGGCAAGGTCAGTATTGAAAAGCTCCATCAAGCTGGCTTCTTTACTTGTAAGGACATTAAGGAAT
CTGACTATCGAGATCTATTGCTCCGGTTTGGCCGTCAAGGCGCATCACTCTGGAAGCGCAGCCATGGTATTGATGACAGA
GAAGTTATTATTGAGCGTGAACGAAAATCAGTAGGCGTAGAGCGAACTTTTACTCAGAATATTTCTACTTACGCAGAATG
TTGGCAGGTAATAGAAGACAAGCTTTTTCCTGAGCTTGAAGCACGCCTAGAGAAAGCTAGCCCAACTAAAGCTATCATCA
AGCAGGGGATTAAGCTCAAGTTTGCCGACTTCCAGCAAACCACTATTGAGCACATACACGCCTCTCTCGATCGAGAGCAC
TTTAAAGAACTGTTGAGTGAGATACTCAAAAGGCAGCAAGGGCGAGAAATACGCCTACTTGGTTTAAGCGTAATGCTACA
ACCCAAAGACCAAATGCGTCAATTGAGTTTCTTTTAG

Upstream 100 bases:

>100_bases
TGAAGTTAAAGCTATTTAGCTGGGCTCCAGATCATTTTAAATCTGAGAATAATTTATTATACTGTTTATAAATACAGTTG
TAAGGTTGAGTTATTCCTAG

Downstream 100 bases:

>100_bases
GGAAAGGCGCTGAGAGTTTAGATTTAGAGGTGTTGCTGGAAGTGTTAGAGTTTGAATAGCGACTAGACTCCACAGCCTAG
CCAGTTCTATATCAATACGT

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MCSAGEEKVRKIIHVDMDCFYAAVEMRDNPAYRNRPLAVGGHEKQRGVLSTCNYEARKFGIRSAMPTGKALQLCPNLLVV
PGRMSVYVEISKKIREIFSRYTSIIEPLSLDEAFLDVTDSTLCHGSATLIAESIRRDIWNELNLTASAGAAPIKFLAKVA
SDLNKPNGQFVIPPQDVQSVIDELPLEKIPGVGKVSIEKLHQAGFFTCKDIKESDYRDLLLRFGRQGASLWKRSHGIDDR
EVIIERERKSVGVERTFTQNISTYAECWQVIEDKLFPELEARLEKASPTKAIIKQGIKLKFADFQQTTIEHIHASLDREH
FKELLSEILKRQQGREIRLLGLSVMLQPKDQMRQLSFF

Sequences:

>Translated_358_residues
MCSAGEEKVRKIIHVDMDCFYAAVEMRDNPAYRNRPLAVGGHEKQRGVLSTCNYEARKFGIRSAMPTGKALQLCPNLLVV
PGRMSVYVEISKKIREIFSRYTSIIEPLSLDEAFLDVTDSTLCHGSATLIAESIRRDIWNELNLTASAGAAPIKFLAKVA
SDLNKPNGQFVIPPQDVQSVIDELPLEKIPGVGKVSIEKLHQAGFFTCKDIKESDYRDLLLRFGRQGASLWKRSHGIDDR
EVIIERERKSVGVERTFTQNISTYAECWQVIEDKLFPELEARLEKASPTKAIIKQGIKLKFADFQQTTIEHIHASLDREH
FKELLSEILKRQQGREIRLLGLSVMLQPKDQMRQLSFF
>Mature_358_residues
MCSAGEEKVRKIIHVDMDCFYAAVEMRDNPAYRNRPLAVGGHEKQRGVLSTCNYEARKFGIRSAMPTGKALQLCPNLLVV
PGRMSVYVEISKKIREIFSRYTSIIEPLSLDEAFLDVTDSTLCHGSATLIAESIRRDIWNELNLTASAGAAPIKFLAKVA
SDLNKPNGQFVIPPQDVQSVIDELPLEKIPGVGKVSIEKLHQAGFFTCKDIKESDYRDLLLRFGRQGASLWKRSHGIDDR
EVIIERERKSVGVERTFTQNISTYAECWQVIEDKLFPELEARLEKASPTKAIIKQGIKLKFADFQQTTIEHIHASLDREH
FKELLSEILKRQQGREIRLLGLSVMLQPKDQMRQLSFF

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI7705344, Length=431, Percent_Identity=31.0904872389791, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI154350220, Length=303, Percent_Identity=31.6831683168317, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI84043967, Length=300, Percent_Identity=30.3333333333333, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI7706681, Length=301, Percent_Identity=30.2325581395349, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI5729982, Length=126, Percent_Identity=38.0952380952381, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1786425, Length=351, Percent_Identity=60.6837606837607, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1787432, Length=210, Percent_Identity=25.7142857142857, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193205700, Length=403, Percent_Identity=30.5210918114144, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17537959, Length=307, Percent_Identity=27.3615635179153, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193205702, Length=348, Percent_Identity=27.0114942528736, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115534089, Length=326, Percent_Identity=25.7668711656442, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324921, Length=205, Percent_Identity=27.3170731707317, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19923006, Length=314, Percent_Identity=30.2547770700637, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21355641, Length=288, Percent_Identity=28.8194444444444, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644984, Length=288, Percent_Identity=28.8194444444444, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24668444, Length=119, Percent_Identity=37.8151260504202, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40632; Mature: 40632

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSAGEEKVRKIIHVDMDCFYAAVEMRDNPAYRNRPLAVGGHEKQRGVLSTCNYEARKFG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHCCHHHHCCHHHHHHH
IRSAMPTGKALQLCPNLLVVPGRMSVYVEISKKIREIFSRYTSIIEPLSLDEAFLDVTDS
HHHCCCCCHHHHHCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
TLCHGSATLIAESIRRDIWNELNLTASAGAAPIKFLAKVASDLNKPNGQFVIPPQDVQSV
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHH
IDELPLEKIPGVGKVSIEKLHQAGFFTCKDIKESDYRDLLLRFGRQGASLWKRSHGIDDR
HHHCCHHHCCCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHH
EVIIERERKSVGVERTFTQNISTYAECWQVIEDKLFPELEARLEKASPTKAIIKQGIKLK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHCCCEEE
FADFQQTTIEHIHASLDREHFKELLSEILKRQQGREIRLLGLSVMLQPKDQMRQLSFF
ECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHHHHHHCCC
>Mature Secondary Structure
MCSAGEEKVRKIIHVDMDCFYAAVEMRDNPAYRNRPLAVGGHEKQRGVLSTCNYEARKFG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHCCHHHHCCHHHHHHH
IRSAMPTGKALQLCPNLLVVPGRMSVYVEISKKIREIFSRYTSIIEPLSLDEAFLDVTDS
HHHCCCCCHHHHHCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
TLCHGSATLIAESIRRDIWNELNLTASAGAAPIKFLAKVASDLNKPNGQFVIPPQDVQSV
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHH
IDELPLEKIPGVGKVSIEKLHQAGFFTCKDIKESDYRDLLLRFGRQGASLWKRSHGIDDR
HHHCCHHHCCCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHH
EVIIERERKSVGVERTFTQNISTYAECWQVIEDKLFPELEARLEKASPTKAIIKQGIKLK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHCCCEEE
FADFQQTTIEHIHASLDREHFKELLSEILKRQQGREIRLLGLSVMLQPKDQMRQLSFF
ECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]