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The map label for this gene is nqrB [H]

Identifier: 218708715

GI number: 218708715

Start: 728470

End: 729714

Strand: Direct

Name: nqrB [H]

Synonym: VS_0694

Alternate gene names: 218708715

Gene position: 728470-729714 (Clockwise)

Preceding gene: 218708714

Following gene: 218708716

Centisome position: 22.08

GC content: 47.39

Gene sequence:

>1245_bases
ATGGGCCTTAAAAAGTTTCTTGAAGACATCGAGCATCATTTTGAGCCAGGTGGTAAACACGAGAAGTGGTTTGCGCTTTA
CGAAGCAGCAGCGACAGTGTTTTACACGCCAGGTCTTATCACAAAGAAAAGCTCACACGTTCGTGATAGCGTTGATTTAA
AACGTATCATGATCATGGTTTGGTTCGCGGTATTTCCAGCAATGTTCTGGGGTATGTACAACGCGGGTGGCCAAGCTATC
GCTGCACTTAACCATATGTACGCAGGCGCTGAACTAGCATCTGTTATCAGTGGTAACTGGCACTACTGGCTAACCGAAAT
GCTTGGAGCCTCCTTAGGGCCCGATGCAGGTGTTGGCAGCAAGATGCTACTTGGTGCGACATACTTCCTACCTATCTATG
CAACGGTATTCATCGTTGGTGGTTTCTGGGAAGTTCTGTTCTGTATGGTGCGTAAGCACGAAGTAAACGAAGGTTTCTTT
GTTACTTCTATCTTATTTGCGCTTATCGTTCCGCCAACGCTTCCTCTATGGCAAGCAGCACTAGGTATTACCTTCGGTGT
TGTTGTGGCGAAAGAGATCTTCGGTGGTACGGGTCGTAACTTCCTGAACCCTGCACTTGCTGGCCGTGCGTTCCTATTCT
TTGCATACCCTGCACAGATTTCAGGTGACGTAGTTTGGACTGCTGCAGATGGTTTCTCTGGTGCAACTGCTCTTAGCCAA
TGGGCTCAAGGCGGCGGTAGCGCACTAATGAACGTGACATCAGGTGAAGCAATCACTTGGATGGACGCATTCATTGGTAA
CATCCCAGGTTCTATCGGTGAAGTATCAACTCTAGCACTTATGATTGGTGCAGCGATGATCGTTTACATGCGTATCGCTT
CATGGCGCATCATTGCTGGTGTAATGATCGGTATGATTGCTGTGTCTACACTGTTCAACGTGATCGGTTCTGATACTAAC
GCAATGTTCAGCATGCCTTGGCATTGGCACCTAGTTCTAGGTGGCTTCGCATTCGGTATGTTCTTCATGGCGACTGACCC
AGTATCAGCTTCATTTACCAATAAAGGTAAGTGGTGGTACGGCATCCTAATCGGCGCAATGTGTGTAATGATTCGTGTAG
TTAACCCTGCATACCCAGAAGGCATGATGCTTGCGATTCTATTCGCAAACCTATTTGCTCCTCTGTTTGACCACGTTGTA
ATCGAGAAGAACATTAAGCGGAGACTAGCGCGCTATGGCAAGTAA

Upstream 100 bases:

>100_bases
AAGATGTAGCATTGTGTACCTTTGTATGTCCAGGTAAGTACGAGTACGGTCAACTACTTCGTGAATGCCTAGATACGATT
GAGAAGGAAGGGTAATTTTC

Downstream 100 bases:

>100_bases
TAACGATAGCATTAAAAAGACGCTGTTTGTTGTTATCGCATTGAGCCTAGTGTGCTCAATCATCGTTTCAACAGCTGCAG
TTGTTCTTAAACCTAAGCAG

Product: Na(+)-translocating NADH-quinone reductase subunit B

Products: NA

Alternate protein names: Na(+)-NQR subunit B; Na(+)-translocating NQR subunit B; NQR complex subunit B; NQR-1 subunit B [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MGLKKFLEDIEHHFEPGGKHEKWFALYEAAATVFYTPGLITKKSSHVRDSVDLKRIMIMVWFAVFPAMFWGMYNAGGQAI
AALNHMYAGAELASVISGNWHYWLTEMLGASLGPDAGVGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFF
VTSILFALIVPPTLPLWQAALGITFGVVVAKEIFGGTGRNFLNPALAGRAFLFFAYPAQISGDVVWTAADGFSGATALSQ
WAQGGGSALMNVTSGEAITWMDAFIGNIPGSIGEVSTLALMIGAAMIVYMRIASWRIIAGVMIGMIAVSTLFNVIGSDTN
AMFSMPWHWHLVLGGFAFGMFFMATDPVSASFTNKGKWWYGILIGAMCVMIRVVNPAYPEGMMLAILFANLFAPLFDHVV
IEKNIKRRLARYGK

Sequences:

>Translated_414_residues
MGLKKFLEDIEHHFEPGGKHEKWFALYEAAATVFYTPGLITKKSSHVRDSVDLKRIMIMVWFAVFPAMFWGMYNAGGQAI
AALNHMYAGAELASVISGNWHYWLTEMLGASLGPDAGVGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFF
VTSILFALIVPPTLPLWQAALGITFGVVVAKEIFGGTGRNFLNPALAGRAFLFFAYPAQISGDVVWTAADGFSGATALSQ
WAQGGGSALMNVTSGEAITWMDAFIGNIPGSIGEVSTLALMIGAAMIVYMRIASWRIIAGVMIGMIAVSTLFNVIGSDTN
AMFSMPWHWHLVLGGFAFGMFFMATDPVSASFTNKGKWWYGILIGAMCVMIRVVNPAYPEGMMLAILFANLFAPLFDHVV
IEKNIKRRLARYGK
>Mature_413_residues
GLKKFLEDIEHHFEPGGKHEKWFALYEAAATVFYTPGLITKKSSHVRDSVDLKRIMIMVWFAVFPAMFWGMYNAGGQAIA
ALNHMYAGAELASVISGNWHYWLTEMLGASLGPDAGVGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFV
TSILFALIVPPTLPLWQAALGITFGVVVAKEIFGGTGRNFLNPALAGRAFLFFAYPAQISGDVVWTAADGFSGATALSQW
AQGGGSALMNVTSGEAITWMDAFIGNIPGSIGEVSTLALMIGAAMIVYMRIASWRIIAGVMIGMIAVSTLFNVIGSDTNA
MFSMPWHWHLVLGGFAFGMFFMATDPVSASFTNKGKWWYGILIGAMCVMIRVVNPAYPEGMMLAILFANLFAPLFDHVVI
EKNIKRRLARYGK

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. NqrA to nqrE are probably involved in the second step, the conversion of ubisemiqui

COG id: COG1805

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrB/rnfD family [H]

Homologues:

Organism=Escherichia coli, GI1787917, Length=292, Percent_Identity=26.7123287671233, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010966
- InterPro:   IPR004338 [H]

Pfam domain/function: PF03116 NQR2_RnfD_RnfE [H]

EC number: NA

Molecular weight: Translated: 45238; Mature: 45107

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKKFLEDIEHHFEPGGKHEKWFALYEAAATVFYTPGLITKKSSHVRDSVDLKRIMIMV
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHH
WFAVFPAMFWGMYNAGGQAIAALNHMYAGAELASVISGNWHYWLTEMLGASLGPDAGVGS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCC
KMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFALIVPPTLPLWQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHH
LGITFGVVVAKEIFGGTGRNFLNPALAGRAFLFFAYPAQISGDVVWTAADGFSGATALSQ
HHHHHHHHHHHHHHCCCCCHHCCHHHHCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHH
WAQGGGSALMNVTSGEAITWMDAFIGNIPGSIGEVSTLALMIGAAMIVYMRIASWRIIAG
HHCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMIGMIAVSTLFNVIGSDTNAMFSMPWHWHLVLGGFAFGMFFMATDPVSASFTNKGKWWY
HHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
GILIGAMCVMIRVVNPAYPEGMMLAILFANLFAPLFDHVVIEKNIKRRLARYGK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GLKKFLEDIEHHFEPGGKHEKWFALYEAAATVFYTPGLITKKSSHVRDSVDLKRIMIMV
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHH
WFAVFPAMFWGMYNAGGQAIAALNHMYAGAELASVISGNWHYWLTEMLGASLGPDAGVGS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCC
KMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFALIVPPTLPLWQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHH
LGITFGVVVAKEIFGGTGRNFLNPALAGRAFLFFAYPAQISGDVVWTAADGFSGATALSQ
HHHHHHHHHHHHHHCCCCCHHCCHHHHCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHH
WAQGGGSALMNVTSGEAITWMDAFIGNIPGSIGEVSTLALMIGAAMIVYMRIASWRIIAG
HHCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMIGMIAVSTLFNVIGSDTNAMFSMPWHWHLVLGGFAFGMFFMATDPVSASFTNKGKWWY
HHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
GILIGAMCVMIRVVNPAYPEGMMLAILFANLFAPLFDHVVIEKNIKRRLARYGK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10077658; 10952301 [H]