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The map label for this gene is rotA [H]

Identifier: 218708710

GI number: 218708710

Start: 723786

End: 724334

Strand: Reverse

Name: rotA [H]

Synonym: VS_0689

Alternate gene names: 218708710

Gene position: 724334-723786 (Counterclockwise)

Preceding gene: 218708711

Following gene: 218708709

Centisome position: 21.95

GC content: 46.27

Gene sequence:

>549_bases
ATGGGACGCATTCTATCTTCTATTGCATTGATGTTGGTGAGCGTGAGCGTTTGGGCTGGTCCTAAAGTGAACGTAGAAAC
AACATTAGGCGGCTTCACTATCGAGCTAAACCAAGAGCAAGCACCGGTTAGTGTTGATAACTTTCTGAAATATGTTGCTG
ACGGTAGCTACGAAGGCACTCAGTTTCACCGTGTGATCCCGGGCTTTATGGCTCAGGGTGGTGGCTTCGATCAAGATATG
AAGCAATTAGCAACTTATGCGCCTATCAAAAATGAAGGCAGCAATGGCCTGAAGAATGATACTGCAACGATCGCAATGGC
TCGTACCAATGCACCAGATTCTGCAACTCGCCAGTTCTTCATTAACTTTGCAGACAACGATTTCTTAAACGCGAAAGGCG
GCAATCCTGGTTACGCTGTGTTTGGTAAAGTAACCGATGGTTTTGACGTTGTTCAGAAGATGGCAACCATTCCAACCAAG
CGAATGGGCCGCATGTCAGACATTCCTGTCGACCCAATCGTCATCACTAAAGTGACCCTTCTTAAGTAA

Upstream 100 bases:

>100_bases
ATGACGATATCGCAACGGTACTAAACGATGTTATCGACTTAGTTCTTAAAGAGATTGCCAATGACCAAGAGCTGCAAACT
TACATGAAGGAACGTTTCTA

Downstream 100 bases:

>100_bases
TCCAATGTAACGCCCTTATTCTTAAGGGCGTTTTCTATACAAGGACGCCCCATGTCATCAGGCACCCCCTCTCTTTCTTG
GATGCAAACTTTCCGCAGCT

Product: peptidyl-prolyl cis-trans isomerase A

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MGRILSSIALMLVSVSVWAGPKVNVETTLGGFTIELNQEQAPVSVDNFLKYVADGSYEGTQFHRVIPGFMAQGGGFDQDM
KQLATYAPIKNEGSNGLKNDTATIAMARTNAPDSATRQFFINFADNDFLNAKGGNPGYAVFGKVTDGFDVVQKMATIPTK
RMGRMSDIPVDPIVITKVTLLK

Sequences:

>Translated_182_residues
MGRILSSIALMLVSVSVWAGPKVNVETTLGGFTIELNQEQAPVSVDNFLKYVADGSYEGTQFHRVIPGFMAQGGGFDQDM
KQLATYAPIKNEGSNGLKNDTATIAMARTNAPDSATRQFFINFADNDFLNAKGGNPGYAVFGKVTDGFDVVQKMATIPTK
RMGRMSDIPVDPIVITKVTLLK
>Mature_181_residues
GRILSSIALMLVSVSVWAGPKVNVETTLGGFTIELNQEQAPVSVDNFLKYVADGSYEGTQFHRVIPGFMAQGGGFDQDMK
QLATYAPIKNEGSNGLKNDTATIAMARTNAPDSATRQFFINFADNDFLNAKGGNPGYAVFGKVTDGFDVVQKMATIPTKR
MGRMSDIPVDPIVITKVTLLK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7706339, Length=162, Percent_Identity=31.4814814814815, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI19557636, Length=158, Percent_Identity=30.379746835443, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI22547212, Length=161, Percent_Identity=29.1925465838509, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI7657473, Length=161, Percent_Identity=29.1925465838509, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI22547215, Length=161, Percent_Identity=29.1925465838509, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI64276486, Length=133, Percent_Identity=36.0902255639098, Blast_Score=67, Evalue=8e-12,
Organism=Escherichia coli, GI1789763, Length=181, Percent_Identity=48.0662983425414, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1786736, Length=161, Percent_Identity=52.1739130434783, Blast_Score=159, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI71980590, Length=144, Percent_Identity=36.1111111111111, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71980594, Length=158, Percent_Identity=34.8101265822785, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17506311, Length=162, Percent_Identity=31.4814814814815, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539496, Length=146, Percent_Identity=31.5068493150685, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532641, Length=162, Percent_Identity=25.3086419753086, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17986117, Length=167, Percent_Identity=31.1377245508982, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922376, Length=168, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24586038, Length=143, Percent_Identity=32.1678321678322, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 19630; Mature: 19499

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRILSSIALMLVSVSVWAGPKVNVETTLGGFTIELNQEQAPVSVDNFLKYVADGSYEGT
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCCCCCHHHHHHHHHCCCCCCC
QFHRVIPGFMAQGGGFDQDMKQLATYAPIKNEGSNGLKNDTATIAMARTNAPDSATRQFF
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEE
INFADNDFLNAKGGNPGYAVFGKVTDGFDVVQKMATIPTKRMGRMSDIPVDPIVITKVTL
EEECCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEEEE
LK
CC
>Mature Secondary Structure 
GRILSSIALMLVSVSVWAGPKVNVETTLGGFTIELNQEQAPVSVDNFLKYVADGSYEGT
CHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCCCCCHHHHHHHHHCCCCCCC
QFHRVIPGFMAQGGGFDQDMKQLATYAPIKNEGSNGLKNDTATIAMARTNAPDSATRQFF
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEE
INFADNDFLNAKGGNPGYAVFGKVTDGFDVVQKMATIPTKRMGRMSDIPVDPIVITKVTL
EEECCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEEEE
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]