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The map label for this gene is smpB [H]

Identifier: 218708663

GI number: 218708663

Start: 669229

End: 669783

Strand: Reverse

Name: smpB [H]

Synonym: VS_0642

Alternate gene names: 218708663

Gene position: 669783-669229 (Counterclockwise)

Preceding gene: 218708666

Following gene: 218708658

Centisome position: 20.3

GC content: 44.32

Gene sequence:

>555_bases
TTGTATTGGGCCAGAGCGCAAATAAAAAGGATTCCCCAACTAGGGTGCAATCCGTATAATGAGGCCATTATGGCAAAGAA
TAAATCAAAATCAAAAGCCGGTAGCAATACCATTGCGCTTAATAAGAAAGCTCGCCACGAATATTTCATCGATGATGAGA
TAGAAGCGGGGCTTGAGCTACAAGGCTGGGAAGTAAAATCCCTACGTGAAGGCAAAACCAATATCGCAGAAAGCTACGTC
TACATCCGAGACGGCGAAGCATTCATCAGTGGTATGACGATCACTCCGCTAACTCAAGCGAGTACTCATATCGTGGCGAA
CCCAACACGCATCCGTAAACTACTAATGTCGAGAAAAGAACTCGACAACCTTATCGGTCGTATTAACCGTGAAGGCATGA
CGCTTGTCGCAACCGCACTTTATTGGTCTCGCTCTTGGGCGAAGATTAAAGTTGGCGTAGCGAAAGGCAAAAAGCTGCAC
GATAAACGTACTGATATGAAAGAAAAAGATTGGGCGAGAGATAAAGCACGAATTATGAAGAGTAATTTGCGTTAA

Upstream 100 bases:

>100_bases
ATCTGGTCGGCACTAAACGACACTAATGCTGAACGAGTAACCTTTGGCATATAGACTCCTGTTAAAGACAGCGTTACATT
AACACAAACTGCAATTTTAA

Downstream 100 bases:

>100_bases
TTTAAGCACTTAAACGCACAGTGCTAGACAGGCAAAGCTTTTCTGGTACTATGCAAATAACACTTGGGGCTGATTTAGGA
TTCGACAGGAATTTTGAAGT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MYWARAQIKRIPQLGCNPYNEAIMAKNKSKSKAGSNTIALNKKARHEYFIDDEIEAGLELQGWEVKSLREGKTNIAESYV
YIRDGEAFISGMTITPLTQASTHIVANPTRIRKLLMSRKELDNLIGRINREGMTLVATALYWSRSWAKIKVGVAKGKKLH
DKRTDMKEKDWARDKARIMKSNLR

Sequences:

>Translated_184_residues
MYWARAQIKRIPQLGCNPYNEAIMAKNKSKSKAGSNTIALNKKARHEYFIDDEIEAGLELQGWEVKSLREGKTNIAESYV
YIRDGEAFISGMTITPLTQASTHIVANPTRIRKLLMSRKELDNLIGRINREGMTLVATALYWSRSWAKIKVGVAKGKKLH
DKRTDMKEKDWARDKARIMKSNLR
>Mature_184_residues
MYWARAQIKRIPQLGCNPYNEAIMAKNKSKSKAGSNTIALNKKARHEYFIDDEIEAGLELQGWEVKSLREGKTNIAESYV
YIRDGEAFISGMTITPLTQASTHIVANPTRIRKLLMSRKELDNLIGRINREGMTLVATALYWSRSWAKIKVGVAKGKKLH
DKRTDMKEKDWARDKARIMKSNLR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=65.2173913043478, Blast_Score=224, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 21068; Mature: 21068

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYWARAQIKRIPQLGCNPYNEAIMAKNKSKSKAGSNTIALNKKARHEYFIDDEIEAGLEL
CCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHCCCCEE
QGWEVKSLREGKTNIAESYVYIRDGEAFISGMTITPLTQASTHIVANPTRIRKLLMSRKE
CCCCHHHHHCCCCCHHHCEEEEECCHHEEECCEECCCCCCCCEEEECHHHHHHHHHHHHH
LDNLIGRINREGMTLVATALYWSRSWAKIKVGVAKGKKLHDKRTDMKEKDWARDKARIMK
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
SNLR
HCCC
>Mature Secondary Structure
MYWARAQIKRIPQLGCNPYNEAIMAKNKSKSKAGSNTIALNKKARHEYFIDDEIEAGLEL
CCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHCCCCEE
QGWEVKSLREGKTNIAESYVYIRDGEAFISGMTITPLTQASTHIVANPTRIRKLLMSRKE
CCCCHHHHHCCCCCHHHCEEEEECCHHEEECCEECCCCCCCCEEEECHHHHHHHHHHHHH
LDNLIGRINREGMTLVATALYWSRSWAKIKVGVAKGKKLHDKRTDMKEKDWARDKARIMK
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
SNLR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA