Click here to switch to the map view.

The map label for this gene is pheA [H]

Identifier: 218708590

GI number: 218708590

Start: 581317

End: 582492

Strand: Reverse

Name: pheA [H]

Synonym: VS_0556

Alternate gene names: 218708590

Gene position: 582492-581317 (Counterclockwise)

Preceding gene: 218708591

Following gene: 218708589

Centisome position: 17.66

GC content: 43.11

Gene sequence:

>1176_bases
ATGACTGACCGAACAATTTCACTAGATGATATCCGCCTTCGTCTCAATGATTTAGACGACGAACTCCTAAAATTACTTTC
AGAACGTCGTAAGCTGAGTATCGAAGTCGCTAAAAGCAAGGTGGAAACATCAAAACCCGTTCGTGATGCAGTACGAGAGC
AACAACTACTAGTCAAATTGATCAACAACGGTAAAGACAAGTACGAATTGGATGCTCAGTACATTACTAAGCTGTTTCAC
ACCATCATCGAAGATTCCGTTTTACTGCAGCAGTCATACTTACAAAATCTAGCGAATCCGCAAAGTCGTAAACCACTAGC
TCGCGTTGCATTCTTAGGTTCTAAGGGATCGTATTCTCACCTTGCGAGCCGTGAATACTTCAGCCGCAAGAATATGGAAT
TGATTGAGCTGAACTGTAATCACTTCAAAGAAGTCGCATCGACTGTAGAATCGGGCCATGCCGACTACGGTGTACTGCCG
ATTGAAAATACCAGTTCAGGGTCAATTAACGAAGTCTACGATCTGCTACAACACACAACGCTTTACATCGTTGGTGAACT
TTCTCAACCGATTGAACATTGCCTTGTCGCGAAAAATGATATTCGTTTAGAAGACATTAAAACGCTCTATTCGCACCCAC
AACCTCATCAACAATGCAGTGAGTTTTTAAGCCGCCTTAAAGATGTCAGCCTTGAATCTTGTGCAAGCACAGCTGATGCC
ATGAAAAAGGTAAAAGATCTAGACGGTGATGATGTGGCAGCGATTGGTAATGCATCGAGCGGCAAGCTTTATGGACTTCA
ACCAATCCAAGGCAACATTGCAAATCAAACTGAGAACCACACTCGTTTCATCGTGGTCGCTCGTAAGCCTGTTGAAGTAT
CGACTCAGATCCCAGCTAAGACGACACTCATCATGTCGACTTCTCAAGAAGCCGGTTCTTTGGTTGAAACACTGCTGATT
CTGCAACGCTTAGGTATTAACATGACCAAGCTGGAATCTCGTCCTATCATGGGTAACCCTTGGGAAGAGATGTTCTATGT
GGATTTAGAAGCACACTTAGATTCAGATAATATGCAGCAAGCGATAACGGAACTAACAGCCATAACTCGTCACCTAAAAG
TGTTAGGTTGTTACCCAAGTGAGAACATTAAGGCTACTCAGGTTAAGTTGTCTTAA

Upstream 100 bases:

>100_bases
TTTCTTCCGTAAATCGGAGGCTAAGTCGTTTTAGAAAAACAAGTCAAAAACGAACAAGAAGCCTCCCACACTAGGGGGGC
TTTTTTATAAGGACACATTT

Downstream 100 bases:

>100_bases
CTTTTTATTATCTGTTCCCCCATTTTAACTGCTATAAGCTTTTCACTAATACAAGCATTTGGCCTACTGCAGGCCAAATG
CTAAAAGAAAGAACTAAGGA

Product: bifunctional chorismate mutase/prephenatedehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTDRTISLDDIRLRLNDLDDELLKLLSERRKLSIEVAKSKVETSKPVRDAVREQQLLVKLINNGKDKYELDAQYITKLFH
TIIEDSVLLQQSYLQNLANPQSRKPLARVAFLGSKGSYSHLASREYFSRKNMELIELNCNHFKEVASTVESGHADYGVLP
IENTSSGSINEVYDLLQHTTLYIVGELSQPIEHCLVAKNDIRLEDIKTLYSHPQPHQQCSEFLSRLKDVSLESCASTADA
MKKVKDLDGDDVAAIGNASSGKLYGLQPIQGNIANQTENHTRFIVVARKPVEVSTQIPAKTTLIMSTSQEAGSLVETLLI
LQRLGINMTKLESRPIMGNPWEEMFYVDLEAHLDSDNMQQAITELTAITRHLKVLGCYPSENIKATQVKLS

Sequences:

>Translated_391_residues
MTDRTISLDDIRLRLNDLDDELLKLLSERRKLSIEVAKSKVETSKPVRDAVREQQLLVKLINNGKDKYELDAQYITKLFH
TIIEDSVLLQQSYLQNLANPQSRKPLARVAFLGSKGSYSHLASREYFSRKNMELIELNCNHFKEVASTVESGHADYGVLP
IENTSSGSINEVYDLLQHTTLYIVGELSQPIEHCLVAKNDIRLEDIKTLYSHPQPHQQCSEFLSRLKDVSLESCASTADA
MKKVKDLDGDDVAAIGNASSGKLYGLQPIQGNIANQTENHTRFIVVARKPVEVSTQIPAKTTLIMSTSQEAGSLVETLLI
LQRLGINMTKLESRPIMGNPWEEMFYVDLEAHLDSDNMQQAITELTAITRHLKVLGCYPSENIKATQVKLS
>Mature_390_residues
TDRTISLDDIRLRLNDLDDELLKLLSERRKLSIEVAKSKVETSKPVRDAVREQQLLVKLINNGKDKYELDAQYITKLFHT
IIEDSVLLQQSYLQNLANPQSRKPLARVAFLGSKGSYSHLASREYFSRKNMELIELNCNHFKEVASTVESGHADYGVLPI
ENTSSGSINEVYDLLQHTTLYIVGELSQPIEHCLVAKNDIRLEDIKTLYSHPQPHQQCSEFLSRLKDVSLESCASTADAM
KKVKDLDGDDVAAIGNASSGKLYGLQPIQGNIANQTENHTRFIVVARKPVEVSTQIPAKTTLIMSTSQEAGSLVETLLIL
QRLGINMTKLESRPIMGNPWEEMFYVDLEAHLDSDNMQQAITELTAITRHLKVLGCYPSENIKATQVKLS

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=376, Percent_Identity=55.3191489361702, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6324013, Length=324, Percent_Identity=24.0740740740741, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 43895; Mature: 43763

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRTISLDDIRLRLNDLDDELLKLLSERRKLSIEVAKSKVETSKPVRDAVREQQLLVKL
CCCCEECHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
INNGKDKYELDAQYITKLFHTIIEDSVLLQQSYLQNLANPQSRKPLARVAFLGSKGSYSH
HHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCHHH
LASREYFSRKNMELIELNCNHFKEVASTVESGHADYGVLPIENTSSGSINEVYDLLQHTT
HHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
LYIVGELSQPIEHCLVAKNDIRLEDIKTLYSHPQPHQQCSEFLSRLKDVSLESCASTADA
EEEEECHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
MKKVKDLDGDDVAAIGNASSGKLYGLQPIQGNIANQTENHTRFIVVARKPVEVSTQIPAK
HHHHHCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCE
TTLIMSTSQEAGSLVETLLILQRLGINMTKLESRPIMGNPWEEMFYVDLEAHLDSDNMQQ
EEEEEECCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHEEEEEEEHHCCCHHHHH
AITELTAITRHLKVLGCYPSENIKATQVKLS
HHHHHHHHHHHHHHEECCCCCCCEEEEEEEC
>Mature Secondary Structure 
TDRTISLDDIRLRLNDLDDELLKLLSERRKLSIEVAKSKVETSKPVRDAVREQQLLVKL
CCCEECHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
INNGKDKYELDAQYITKLFHTIIEDSVLLQQSYLQNLANPQSRKPLARVAFLGSKGSYSH
HHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCHHH
LASREYFSRKNMELIELNCNHFKEVASTVESGHADYGVLPIENTSSGSINEVYDLLQHTT
HHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
LYIVGELSQPIEHCLVAKNDIRLEDIKTLYSHPQPHQQCSEFLSRLKDVSLESCASTADA
EEEEECHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
MKKVKDLDGDDVAAIGNASSGKLYGLQPIQGNIANQTENHTRFIVVARKPVEVSTQIPAK
HHHHHCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCE
TTLIMSTSQEAGSLVETLLILQRLGINMTKLESRPIMGNPWEEMFYVDLEAHLDSDNMQQ
EEEEEECCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHEEEEEEEHHCCCHHHHH
AITELTAITRHLKVLGCYPSENIKATQVKLS
HHHHHHHHHHHHHHEECCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1512561; 1444388 [H]