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The map label for this gene is mbaA [H]

Identifier: 218708589

GI number: 218708589

Start: 578602

End: 580980

Strand: Reverse

Name: mbaA [H]

Synonym: VS_0555

Alternate gene names: 218708589

Gene position: 580980-578602 (Counterclockwise)

Preceding gene: 218708590

Following gene: 218708588

Centisome position: 17.61

GC content: 41.07

Gene sequence:

>2379_bases
ATGAAGCTAAGTACTAAAATATTACTTGTGATTGCCCCCGTAATACTGATCAGTACAGCGGCTTCTAGCTATATCATCTA
TTCCACGCAAAAAAACAGCTTTATCAAACGTGAAGACAATGCACTGCAATTAAGCATGGAAAAATTAGCAGGCTATTTTC
AACAGTCTCGTTCTTTCCTCAACAATTACTCCTACACCCTCACCAATAGTGACCTGGTTAAGCGTTATTTTATGGTTGAT
GAAAATGTCGACCACCAACGTGAACTGACTGATAATCTGAACGAAACGATTAAGTTACTGCAAGATGGTAACGACAGTTT
TACAAGGGTCGCGTTGCTTGATAGCGATCGAGCTCTTAAATATTACGCTGACAATACCAATGATCCTTTCGCAAAAATGG
ATCCCAAGATATTAGAGTTCATTGAGCATCAATATCAAAAAACGTTAGCAACTTCGAGTACGGACTACATACAGAATTCC
CAAGGTGAAGGGGTATTAGTTCATTATGATGTTATTGATAAAAAAACAGTTTCGACGCCAATAAACGATCCACCCAACAA
TGTGTTTTTTGTGGTTGTATCTGTCTCTCTTGATAAGTTTAATTCACTACGAAAACAGATAGAGTTCGACTATCAAACTA
GTTTGTTTTTTACCCCTACGTTAGTGACACAGGGGCGTGAGTTAACACAATCTATAAAGCTAGCACCTGGCCTCTACGCC
ACGCTCGATCCTGCACCATTCTTATTAGACAACAAACTCAAATCTATCTGGAATGAACTGAGCCTATCCTTTGCCCTATC
TGCATTTGTCAGTGTTACCTTACTGCTGATCCTGCTATCTCGCTGGGTGATAAACCCGATCACCCGTTTAGATAGGCAGC
TTCAAGAGGTTGAGAAAAAACAGAGAAAAAACATTGAGCGCCTAGGAACAAATGATGAGTTGGGGCGCTTATCCCAACGC
TTTTATGATATGTACCAAGAGTTGGATGACACGTACCAACAGACTAAGTTGTTGGCAGAAAATGATCAGTTGACCCAAAT
AGCCAATAGGCACCAGTTTCAAACCTTCGTGCAGCAGTCGTTGTCAAAACCTAGCTCGAACACCGAAACCTGGGTTCTCT
ACTTTGATTTAGATAACTTCAAATTTGTGAACGACAAATACGGACACCAAATCGGCGACTCGATTCTCGTCAGTTTTGCT
CAGCGTATCTCCAATATTTGTAACCACTACAAAACAGAATTAAATGCCCACTGTCTGCCAGCCCGACTTTCTGGGGACGA
GTTCGTTGTATACCTTAATGCTCCAAATCACCAAGAAAATATCGCACATCGATTTTCGAATGATTTACTCACCCCACTAC
AAAAGGGGTTTGTCACCGAGTCAGGTAATTTCCCGATTACGGTCAGTATTGGTATTGCGACCCACCCTAATGATGGCGAT
ACAATCGAGAAGCTTCTTTCGAATGCTGATACCGCGATGTATCAAGCCAAACGTGCAGGTAAAAACCAATTCGCAGATTA
CTCGTTAGACTTAGATAAAACCATTCAGCGTCAAGCCAGTATTGAGAGCGCCCTCAGAGAAAATAATTTTGATGAAGAGT
TTAAGCTCGTTTACATGCCTTATATGGACTCAACAGGAAGCTATATTATTGGTGTAGAGGTGCTATTACGTTGGCACTCG
AAACACCTTGGTTTGGTGCCACCCGATGAATTCATTCCTATCGCCGAACAAACCGGATTATTTGAAATAATTGATCGCTG
GGTGATCCAGAATGCATTTGCTTCATTCCATCAACTTCAAGCTCAATTCGATCACTCTATTCAGCTATCGATTAACTTAT
CTTCAGCCGGGATTAAAACCACTCACCTTGCTGATTTCATCAAAGAGCATGCAAAAATCAATCAAATCCCGCCCAATTTG
ATCGATTTTGAAATCACAGAAACCTTTTACTCAAACTCTCAGGGGTTCCCATTACTTAACCAGTTATCATACATGGGCTA
TAGATTAGCAATCGACGACTTTGGTTCTGGTTACACCTCGATCACTCAACTGGTTCAATATCCAACTCAGAAGATTAAAT
TGGATAAAACCTTTTTGGACACCTTGATTGAGACAGATAACCAGAACATAGTGAAACCCATTATCGGGCTATGCCATGCT
CAAGGGAAAAAGGTCACAGCCGAAGGGATTGAAACTCAATGCATGCATCAGTGGTTACAAGATGCGCAGTGCGATATGCT
GCAAGGCTATTATTTTGGTAAGCCTATGCCATTAGAAGAGCTGACGGAATGGTACCGACAGCATCAACTCAATTTTATCG
ATAATAAAAAGAATCAACCACATGAAATCTGTAATCATCGCATCGCTGAATCCAGCTAA

Upstream 100 bases:

>100_bases
CCCAAACAAAACTCTTCAAACACAATATAATCGATGGCAAGAACTCTCGAAATAACATAAACTTGCGCGCTAAAATTTAT
AAACCATGTTGTGAATAACC

Downstream 100 bases:

>100_bases
AATTAGCGCCGTTAAAAGTGCATTCTTATCGGCTTTTCCTGATACTGAATTTGATTTCAAAGGCATCAGTGTCCCTAGTG
GCGTCGCCGATCAACCCATG

Product: biofilm architecture maintenance protein mbaA precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 792; Mature: 792

Protein sequence:

>792_residues
MKLSTKILLVIAPVILISTAASSYIIYSTQKNSFIKREDNALQLSMEKLAGYFQQSRSFLNNYSYTLTNSDLVKRYFMVD
ENVDHQRELTDNLNETIKLLQDGNDSFTRVALLDSDRALKYYADNTNDPFAKMDPKILEFIEHQYQKTLATSSTDYIQNS
QGEGVLVHYDVIDKKTVSTPINDPPNNVFFVVVSVSLDKFNSLRKQIEFDYQTSLFFTPTLVTQGRELTQSIKLAPGLYA
TLDPAPFLLDNKLKSIWNELSLSFALSAFVSVTLLLILLSRWVINPITRLDRQLQEVEKKQRKNIERLGTNDELGRLSQR
FYDMYQELDDTYQQTKLLAENDQLTQIANRHQFQTFVQQSLSKPSSNTETWVLYFDLDNFKFVNDKYGHQIGDSILVSFA
QRISNICNHYKTELNAHCLPARLSGDEFVVYLNAPNHQENIAHRFSNDLLTPLQKGFVTESGNFPITVSIGIATHPNDGD
TIEKLLSNADTAMYQAKRAGKNQFADYSLDLDKTIQRQASIESALRENNFDEEFKLVYMPYMDSTGSYIIGVEVLLRWHS
KHLGLVPPDEFIPIAEQTGLFEIIDRWVIQNAFASFHQLQAQFDHSIQLSINLSSAGIKTTHLADFIKEHAKINQIPPNL
IDFEITETFYSNSQGFPLLNQLSYMGYRLAIDDFGSGYTSITQLVQYPTQKIKLDKTFLDTLIETDNQNIVKPIIGLCHA
QGKKVTAEGIETQCMHQWLQDAQCDMLQGYYFGKPMPLEELTEWYRQHQLNFIDNKKNQPHEICNHRIAESS

Sequences:

>Translated_792_residues
MKLSTKILLVIAPVILISTAASSYIIYSTQKNSFIKREDNALQLSMEKLAGYFQQSRSFLNNYSYTLTNSDLVKRYFMVD
ENVDHQRELTDNLNETIKLLQDGNDSFTRVALLDSDRALKYYADNTNDPFAKMDPKILEFIEHQYQKTLATSSTDYIQNS
QGEGVLVHYDVIDKKTVSTPINDPPNNVFFVVVSVSLDKFNSLRKQIEFDYQTSLFFTPTLVTQGRELTQSIKLAPGLYA
TLDPAPFLLDNKLKSIWNELSLSFALSAFVSVTLLLILLSRWVINPITRLDRQLQEVEKKQRKNIERLGTNDELGRLSQR
FYDMYQELDDTYQQTKLLAENDQLTQIANRHQFQTFVQQSLSKPSSNTETWVLYFDLDNFKFVNDKYGHQIGDSILVSFA
QRISNICNHYKTELNAHCLPARLSGDEFVVYLNAPNHQENIAHRFSNDLLTPLQKGFVTESGNFPITVSIGIATHPNDGD
TIEKLLSNADTAMYQAKRAGKNQFADYSLDLDKTIQRQASIESALRENNFDEEFKLVYMPYMDSTGSYIIGVEVLLRWHS
KHLGLVPPDEFIPIAEQTGLFEIIDRWVIQNAFASFHQLQAQFDHSIQLSINLSSAGIKTTHLADFIKEHAKINQIPPNL
IDFEITETFYSNSQGFPLLNQLSYMGYRLAIDDFGSGYTSITQLVQYPTQKIKLDKTFLDTLIETDNQNIVKPIIGLCHA
QGKKVTAEGIETQCMHQWLQDAQCDMLQGYYFGKPMPLEELTEWYRQHQLNFIDNKKNQPHEICNHRIAESS
>Mature_792_residues
MKLSTKILLVIAPVILISTAASSYIIYSTQKNSFIKREDNALQLSMEKLAGYFQQSRSFLNNYSYTLTNSDLVKRYFMVD
ENVDHQRELTDNLNETIKLLQDGNDSFTRVALLDSDRALKYYADNTNDPFAKMDPKILEFIEHQYQKTLATSSTDYIQNS
QGEGVLVHYDVIDKKTVSTPINDPPNNVFFVVVSVSLDKFNSLRKQIEFDYQTSLFFTPTLVTQGRELTQSIKLAPGLYA
TLDPAPFLLDNKLKSIWNELSLSFALSAFVSVTLLLILLSRWVINPITRLDRQLQEVEKKQRKNIERLGTNDELGRLSQR
FYDMYQELDDTYQQTKLLAENDQLTQIANRHQFQTFVQQSLSKPSSNTETWVLYFDLDNFKFVNDKYGHQIGDSILVSFA
QRISNICNHYKTELNAHCLPARLSGDEFVVYLNAPNHQENIAHRFSNDLLTPLQKGFVTESGNFPITVSIGIATHPNDGD
TIEKLLSNADTAMYQAKRAGKNQFADYSLDLDKTIQRQASIESALRENNFDEEFKLVYMPYMDSTGSYIIGVEVLLRWHS
KHLGLVPPDEFIPIAEQTGLFEIIDRWVIQNAFASFHQLQAQFDHSIQLSINLSSAGIKTTHLADFIKEHAKINQIPPNL
IDFEITETFYSNSQGFPLLNQLSYMGYRLAIDDFGSGYTSITQLVQYPTQKIKLDKTFLDTLIETDNQNIVKPIIGLCHA
QGKKVTAEGIETQCMHQWLQDAQCDMLQGYYFGKPMPLEELTEWYRQHQLNFIDNKKNQPHEICNHRIAESS

Specific function: Plays an essential role in the maintenance and the formation of the three-dimensional structure of the biofilms at the later stages of their development. Absence of mbaA promotes the accumulation of larger amount of biomass on the surfaces at later stage

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Single-pass type I membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=441, Percent_Identity=29.7052154195011, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI87081921, Length=456, Percent_Identity=28.2894736842105, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI226510982, Length=526, Percent_Identity=24.5247148288973, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1790496, Length=235, Percent_Identity=34.468085106383, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87081743, Length=257, Percent_Identity=28.0155642023346, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1787055, Length=242, Percent_Identity=30.5785123966942, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87081980, Length=246, Percent_Identity=30.8943089430894, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788502, Length=248, Percent_Identity=26.6129032258064, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786507, Length=241, Percent_Identity=28.6307053941909, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788849, Length=344, Percent_Identity=25.8720930232558, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI87081845, Length=257, Percent_Identity=26.4591439688716, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI87082096, Length=202, Percent_Identity=30.6930693069307, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788381, Length=454, Percent_Identity=22.9074889867841, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=30.2702702702703, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=198, Percent_Identity=29.7979797979798, Blast_Score=73, Evalue=9e-14,
Organism=Escherichia coli, GI1786584, Length=188, Percent_Identity=23.936170212766, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=29.6511627906977, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI87082007, Length=135, Percent_Identity=34.0740740740741, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788956, Length=256, Percent_Identity=25.78125, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=30.5389221556886, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI145693134, Length=168, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1787262, Length=180, Percent_Identity=28.8888888888889, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00672 HAMP [H]

EC number: NA

Molecular weight: Translated: 90851; Mature: 90851

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50885 HAMP ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSTKILLVIAPVILISTAASSYIIYSTQKNSFIKREDNALQLSMEKLAGYFQQSRSFL
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHH
NNYSYTLTNSDLVKRYFMVDENVDHQRELTDNLNETIKLLQDGNDSFTRVALLDSDRALK
HCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
YYADNTNDPFAKMDPKILEFIEHQYQKTLATSSTDYIQNSQGEGVLVHYDVIDKKTVSTP
EEECCCCCCHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCCCCCC
INDPPNNVFFVVVSVSLDKFNSLRKQIEFDYQTSLFFTPTLVTQGRELTQSIKLAPGLYA
CCCCCCCEEEEEEEECHHHHHHHHHHHCCCCCCEEEECHHHHHCCHHHHHHHHCCCCCEE
TLDPAPFLLDNKLKSIWNELSLSFALSAFVSVTLLLILLSRWVINPITRLDRQLQEVEKK
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRKNIERLGTNDELGRLSQRFYDMYQELDDTYQQTKLLAENDQLTQIANRHQFQTFVQQS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LSKPSSNTETWVLYFDLDNFKFVNDKYGHQIGDSILVSFAQRISNICNHYKTELNAHCLP
HCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ARLSGDEFVVYLNAPNHQENIAHRFSNDLLTPLQKGFVTESGNFPITVSIGIATHPNDGD
EEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEEEEEECCCCCH
TIEKLLSNADTAMYQAKRAGKNQFADYSLDLDKTIQRQASIESALRENNFDEEFKLVYMP
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
YMDSTGSYIIGVEVLLRWHSKHLGLVPPDEFIPIAEQTGLFEIIDRWVIQNAFASFHQLQ
CCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHH
AQFDHSIQLSINLSSAGIKTTHLADFIKEHAKINQIPPNLIDFEITETFYSNSQGFPLLN
HHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCCHHH
QLSYMGYRLAIDDFGSGYTSITQLVQYPTQKIKLDKTFLDTLIETDNQNIVKPIIGLCHA
HHHHCCEEEEEECCCCCHHHHHHHHHCCHHHEEEHHHHHHHHHHCCCCHHHHHHHHHHHC
QGKKVTAEGIETQCMHQWLQDAQCDMLQGYYFGKPMPLEELTEWYRQHQLNFIDNKKNQP
CCCEEEHHCHHHHHHHHHHHHCCHHHHCCEECCCCCCHHHHHHHHHHHCCCHHCCCCCCH
HEICNHRIAESS
HHHHCCCCCCCC
>Mature Secondary Structure
MKLSTKILLVIAPVILISTAASSYIIYSTQKNSFIKREDNALQLSMEKLAGYFQQSRSFL
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHH
NNYSYTLTNSDLVKRYFMVDENVDHQRELTDNLNETIKLLQDGNDSFTRVALLDSDRALK
HCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
YYADNTNDPFAKMDPKILEFIEHQYQKTLATSSTDYIQNSQGEGVLVHYDVIDKKTVSTP
EEECCCCCCHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCCCCCCCCC
INDPPNNVFFVVVSVSLDKFNSLRKQIEFDYQTSLFFTPTLVTQGRELTQSIKLAPGLYA
CCCCCCCEEEEEEEECHHHHHHHHHHHCCCCCCEEEECHHHHHCCHHHHHHHHCCCCCEE
TLDPAPFLLDNKLKSIWNELSLSFALSAFVSVTLLLILLSRWVINPITRLDRQLQEVEKK
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRKNIERLGTNDELGRLSQRFYDMYQELDDTYQQTKLLAENDQLTQIANRHQFQTFVQQS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LSKPSSNTETWVLYFDLDNFKFVNDKYGHQIGDSILVSFAQRISNICNHYKTELNAHCLP
HCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ARLSGDEFVVYLNAPNHQENIAHRFSNDLLTPLQKGFVTESGNFPITVSIGIATHPNDGD
EEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEEEEEECCCCCH
TIEKLLSNADTAMYQAKRAGKNQFADYSLDLDKTIQRQASIESALRENNFDEEFKLVYMP
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
YMDSTGSYIIGVEVLLRWHSKHLGLVPPDEFIPIAEQTGLFEIIDRWVIQNAFASFHQLQ
CCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHH
AQFDHSIQLSINLSSAGIKTTHLADFIKEHAKINQIPPNLIDFEITETFYSNSQGFPLLN
HHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCCHHH
QLSYMGYRLAIDDFGSGYTSITQLVQYPTQKIKLDKTFLDTLIETDNQNIVKPIIGLCHA
HHHHCCEEEEEECCCCCHHHHHHHHHCCHHHEEEHHHHHHHHHHCCCCHHHHHHHHHHHC
QGKKVTAEGIETQCMHQWLQDAQCDMLQGYYFGKPMPLEELTEWYRQHQLNFIDNKKNQP
CCCEEEHHCHHHHHHHHHHHHCCHHHHCCEECCCCCCHHHHHHHHHHHCCCHHCCCCCCH
HEICNHRIAESS
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]