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The map label for this gene is yfiC [C]

Identifier: 218708541

GI number: 218708541

Start: 526858

End: 527586

Strand: Reverse

Name: yfiC [C]

Synonym: VS_0507

Alternate gene names: 218708541

Gene position: 527586-526858 (Counterclockwise)

Preceding gene: 218708545

Following gene: 218708540

Centisome position: 15.99

GC content: 45.95

Gene sequence:

>729_bases
ATGAAAGACAAAACGATAAATACTAAAAGCTTCAATTTTAAGAGGTTCTCTATTTACGGAGGACAGAGCGGCATGCCTGT
TAGCACCGATGGAGTACTACTCGGCTCATGGATTAACCTTGCCCCTGAATCATTAGTACTCGATATAGGCACTGGAACAG
GGTTATTAGCCTTAATGGCAGCACAACGTTTTGAGGATGCTTTAATCTCTGCGATCGATATTGATCAGCACGCCATTGAT
GCTGCCACAGTCAATATTGAGCAGTCGCCTTGGCAAGATCGTATCTCCCTTCATCACGACAGCGTGTTAACCACAGATTT
CTCGCAACGATTTGATGCCATAATTTGTAACCCGCCCTACTTCAACTCTGGTGAGCAGGCGCAGCAAAGCCAAAGAGCCA
CCGCGAGACACACCGACAGCTTGGATCACCTTCAACTTGCCCAACGTTGTTTCGAGATAACGACTGATGCCGCCACTGCC
AGTTTTATACTGCCGACGCCCGAAGGAGAAGGCTTCATCAAACTTGCCGAGCAATGTGGTTGGTACCTAGCAAAGCGCCT
TGATGTGAAAACAACCGACAAAAAACCGGCTAGCCGAATTCTATTTGAGTTATCTAAAGATCCTGTTTGCGGGCAAGATT
TGCAGCGCGAATCGCTTACAATTCATCACCAAGGTGGTTATAGCGAAGCATTTATTGCGCTCACTAAAGATTTTTATCTT
AAGATGTAG

Upstream 100 bases:

>100_bases
TCATTGTTAGCAACAATAGATAAGCAATTAGAATATTAATTTACACAAATAGTTAACATTAGAGCTAATCACTTAAAATA
TCTCGATAATCAGAATATTC

Downstream 100 bases:

>100_bases
CAAATACCATGTGATCCTAATCACTAATGCTTCTATAATGTCCGACTACTCTTTTTTATCGTCTGCTTTTTGAGGCAGAA
ACATTTATTGCTTGTGGAGA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA m6A37 methyltransferase

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKDKTINTKSFNFKRFSIYGGQSGMPVSTDGVLLGSWINLAPESLVLDIGTGTGLLALMAAQRFEDALISAIDIDQHAID
AATVNIEQSPWQDRISLHHDSVLTTDFSQRFDAIICNPPYFNSGEQAQQSQRATARHTDSLDHLQLAQRCFEITTDAATA
SFILPTPEGEGFIKLAEQCGWYLAKRLDVKTTDKKPASRILFELSKDPVCGQDLQRESLTIHHQGGYSEAFIALTKDFYL
KM

Sequences:

>Translated_242_residues
MKDKTINTKSFNFKRFSIYGGQSGMPVSTDGVLLGSWINLAPESLVLDIGTGTGLLALMAAQRFEDALISAIDIDQHAID
AATVNIEQSPWQDRISLHHDSVLTTDFSQRFDAIICNPPYFNSGEQAQQSQRATARHTDSLDHLQLAQRCFEITTDAATA
SFILPTPEGEGFIKLAEQCGWYLAKRLDVKTTDKKPASRILFELSKDPVCGQDLQRESLTIHHQGGYSEAFIALTKDFYL
KM
>Mature_242_residues
MKDKTINTKSFNFKRFSIYGGQSGMPVSTDGVLLGSWINLAPESLVLDIGTGTGLLALMAAQRFEDALISAIDIDQHAID
AATVNIEQSPWQDRISLHHDSVLTTDFSQRFDAIICNPPYFNSGEQAQQSQRATARHTDSLDHLQLAQRCFEITTDAATA
SFILPTPEGEGFIKLAEQCGWYLAKRLDVKTTDKKPASRILFELSKDPVCGQDLQRESLTIHHQGGYSEAFIALTKDFYL
KM

Specific function: Specifically methylates the adenine in position 37 of tRNA(Val)

COG id: COG4123

COG function: function code R; Predicted O-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI87082131, Length=241, Percent_Identity=38.5892116182573, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMN6_VIBSL (B7VJ58)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416162.1
- ProteinModelPortal:   B7VJ58
- GeneID:   7160139
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0507
- HOGENOM:   HBG307609
- OMA:   FKQFHIN
- ProtClustDB:   CLSK874062
- GO:   GO:0005737
- HAMAP:   MF_01872
- InterPro:   IPR002052
- InterPro:   IPR007848
- InterPro:   IPR022882

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.55

Molecular weight: Translated: 26860; Mature: 26860

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKTINTKSFNFKRFSIYGGQSGMPVSTDGVLLGSWINLAPESLVLDIGTGTGLLALMA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEHHHHCCCCEEEEECCCCHHHHHHHH
AQRFEDALISAIDIDQHAIDAATVNIEQSPWQDRISLHHDSVLTTDFSQRFDAIICNPPY
HHHHHHHHHHHHCCCHHHCCEEEEECCCCCCHHHHHCCCCCEEECCHHHCCCEEEECCCC
FNSGEQAQQSQRATARHTDSLDHLQLAQRCFEITTDAATASFILPTPEGEGFIKLAEQCG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
WYLAKRLDVKTTDKKPASRILFELSKDPVCGQDLQRESLTIHHQGGYSEAFIALTKDFYL
HHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHEE
KM
CC
>Mature Secondary Structure
MKDKTINTKSFNFKRFSIYGGQSGMPVSTDGVLLGSWINLAPESLVLDIGTGTGLLALMA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEHHHHCCCCEEEEECCCCHHHHHHHH
AQRFEDALISAIDIDQHAIDAATVNIEQSPWQDRISLHHDSVLTTDFSQRFDAIICNPPY
HHHHHHHHHHHHCCCHHHCCEEEEECCCCCCHHHHHCCCCCEEECCHHHCCCEEEECCCC
FNSGEQAQQSQRATARHTDSLDHLQLAQRCFEITTDAATASFILPTPEGEGFIKLAEQCG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
WYLAKRLDVKTTDKKPASRILFELSKDPVCGQDLQRESLTIHHQGGYSEAFIALTKDFYL
HHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHEE
KM
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA