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The map label for this gene is ispB [H]

Identifier: 218708400

GI number: 218708400

Start: 361338

End: 362309

Strand: Reverse

Name: ispB [H]

Synonym: VS_0360

Alternate gene names: 218708400

Gene position: 362309-361338 (Counterclockwise)

Preceding gene: 218708406

Following gene: 218708397

Centisome position: 10.98

GC content: 44.34

Gene sequence:

>972_bases
ATGGATTTTAAAGCTATCCAAACGCTTACTGCCAATGATATGGCAAAAGTGAATGAAACAATTCAAGCCCAACTTAATTC
TGACGTAAGTTTAATCAACCAGCTTGGTTTTTATATCGTTAGCGGTGGTGGCAAACGCCTACGCCCTTTGCTTGCTCTTT
TATCAGCTCGTGCACTTGGTTACCAAGGTGAAGCTCACATCACCTCAGCCGCTTTTATCGAGTTTATTCACACAGCGACC
CTTCTCCATGATGATGTCGTCGATGAGTCGGACATGAGACGCGGCAAAGCGACAGCCAATGCTGCTTTTGGTAATGCAGC
TAGCGTTTTGGTTGGTGATTTTATTTACACCCGCTCATTCCAGATGATGACGACGCTTGGATCTTTAAGGATCCTTGAGC
TAATGAGCGAATCAGTAAATGTGATTGCCGAGGGTGAAGTTCAACAATTGATGAACTGTAATAACCCAGACACCACAGAA
GAAAGCTACATGCAGGTGATCTATTCAAAGACCGCTCGTTTGTTTGAAGCCGCAACCCAAATCGGCGCAATTCTTACAGA
GTCTTCACCTGAGATCGAAACTGCGATGCAAAACTACGGCAAGTATCTAGGAACAGCATTCCAGCTTATTGATGATGTGA
TGGACTACACCGCTGATGGTAAAGAGATGGGTAAAAATGTTGGTGATGACCTAGCAGAAGGCAAACCGACGTTACCATTG
CTTTACGCTATGCATAATGGCTCGCCAGATCAAGCGAGCATGATTCGTGAAGCGATTGAAAAAGCCAATGGTATGGAACG
TCTCGACGACATCATGTCTGTCATGAAAGAGACAGGCTCGTTAGAGTACACGACCAATAAAGCCTATGAAGAAGCTGATA
AAGCCATTGCTGAGCTTTCTATTCTTCCTGAGTCAGAATACAAGCAGGCTTTAACTACGCTAGCTCATCTTGCAGTAAAA
CGTAGCAAGTAA

Upstream 100 bases:

>100_bases
TGCCTTTAAGTGATGTATTAATCAACGTTTTTTAGTGTATTATTCAACAATTAAATACAACGTATAAGCCTTACAGGGTC
TAACTTCAGCCGGATGAACA

Downstream 100 bases:

>100_bases
ATAGAAGCTAAGTAGTAAATAGACAGCTATATAATAAAAAAGAGCCTGTTGGCTCTTTTTTTGATCCTGCTAGATAATAC
ACTGGGTCTCAATAACGACT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDFKAIQTLTANDMAKVNETIQAQLNSDVSLINQLGFYIVSGGGKRLRPLLALLSARALGYQGEAHITSAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRSFQMMTTLGSLRILELMSESVNVIAEGEVQQLMNCNNPDTTE
ESYMQVIYSKTARLFEAATQIGAILTESSPEIETAMQNYGKYLGTAFQLIDDVMDYTADGKEMGKNVGDDLAEGKPTLPL
LYAMHNGSPDQASMIREAIEKANGMERLDDIMSVMKETGSLEYTTNKAYEEADKAIAELSILPESEYKQALTTLAHLAVK
RSK

Sequences:

>Translated_323_residues
MDFKAIQTLTANDMAKVNETIQAQLNSDVSLINQLGFYIVSGGGKRLRPLLALLSARALGYQGEAHITSAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRSFQMMTTLGSLRILELMSESVNVIAEGEVQQLMNCNNPDTTE
ESYMQVIYSKTARLFEAATQIGAILTESSPEIETAMQNYGKYLGTAFQLIDDVMDYTADGKEMGKNVGDDLAEGKPTLPL
LYAMHNGSPDQASMIREAIEKANGMERLDDIMSVMKETGSLEYTTNKAYEEADKAIAELSILPESEYKQALTTLAHLAVK
RSK
>Mature_323_residues
MDFKAIQTLTANDMAKVNETIQAQLNSDVSLINQLGFYIVSGGGKRLRPLLALLSARALGYQGEAHITSAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRSFQMMTTLGSLRILELMSESVNVIAEGEVQQLMNCNNPDTTE
ESYMQVIYSKTARLFEAATQIGAILTESSPEIETAMQNYGKYLGTAFQLIDDVMDYTADGKEMGKNVGDDLAEGKPTLPL
LYAMHNGSPDQASMIREAIEKANGMERLDDIMSVMKETGSLEYTTNKAYEEADKAIAELSILPESEYKQALTTLAHLAVK
RSK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=30.4347826086957, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4758430, Length=288, Percent_Identity=22.9166666666667, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI83700220, Length=288, Percent_Identity=22.9166666666667, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=69.6594427244582, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1786623, Length=229, Percent_Identity=29.2576419213974, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17505681, Length=290, Percent_Identity=33.1034482758621, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=299, Percent_Identity=33.7792642140468, Blast_Score=145, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6325188, Length=292, Percent_Identity=23.2876712328767, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24651612, Length=300, Percent_Identity=30, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI281365769, Length=275, Percent_Identity=23.2727272727273, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24660002, Length=275, Percent_Identity=23.2727272727273, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35342; Mature: 35342

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKAIQTLTANDMAKVNETIQAQLNSDVSLINQLGFYIVSGGGKRLRPLLALLSARALG
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHCC
YQGEAHITSAAFIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRSF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHH
QMMTTLGSLRILELMSESVNVIAEGEVQQLMNCNNPDTTEESYMQVIYSKTARLFEAATQ
HHHHHHHHHHHHHHHHHHHCEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGAILTESSPEIETAMQNYGKYLGTAFQLIDDVMDYTADGKEMGKNVGDDLAEGKPTLPL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCEE
LYAMHNGSPDQASMIREAIEKANGMERLDDIMSVMKETGSLEYTTNKAYEEADKAIAELS
EEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHH
ILPESEYKQALTTLAHLAVKRSK
CCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFKAIQTLTANDMAKVNETIQAQLNSDVSLINQLGFYIVSGGGKRLRPLLALLSARALG
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHCC
YQGEAHITSAAFIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRSF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHH
QMMTTLGSLRILELMSESVNVIAEGEVQQLMNCNNPDTTEESYMQVIYSKTARLFEAATQ
HHHHHHHHHHHHHHHHHHHCEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGAILTESSPEIETAMQNYGKYLGTAFQLIDDVMDYTADGKEMGKNVGDDLAEGKPTLPL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCEE
LYAMHNGSPDQASMIREAIEKANGMERLDDIMSVMKETGSLEYTTNKAYEEADKAIAELS
EEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHH
ILPESEYKQALTTLAHLAVKRSK
CCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]