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The map label for this gene is proC [H]

Identifier: 218703668

GI number: 218703668

Start: 460198

End: 461007

Strand: Reverse

Name: proC [H]

Synonym: ECUMN_0424

Alternate gene names: 218703668

Gene position: 461007-460198 (Counterclockwise)

Preceding gene: 218703675

Following gene: 218703662

Centisome position: 8.86

GC content: 54.69

Gene sequence:

>810_bases
ATGGAAAAGAAAATCGGTTTTATTGGCTGCGGCAATATGGGAAAAGCCATTCTCGGCGGTCTGATTGCCAGCGGTCAGGT
GCTTCCAGGGCAAATCTGGGTATATACCCCCTCCCCGGATAAAGTCGCCGCCCTGCATGACCAGTTCGGCATCAACGCCG
CAGAATCGGCGCAAGAAGTGGCGCAAATCGCCGACATCATTTTTGCCGCCGTTAAACCTGGCATCATGATTAAAGTGCTT
AGCGAAATCACCTCCAGCCTGAATAAAGACTCTCTGGTCGTTTCTATTGCTGCAGGTATCACACTCGACCAGCTTGCCCG
CGCGCTGGGCCATGACCGGAAAATTATCCGCGCCATGCCGAACACTCCCGCGCTGGTTAATGCCGGGATGACCTCCGTAA
CGCCAAACGCGCTGGTAACCCCAGAAGATACCGCTGATGTGCTGAATATTTTCCGCTGCTTTGGCGAAGCGGAAGTAATT
GCCGAGCCGATGATCCACCCGGTGGTCGGTGTGAGCGGTTCTTCGCCAGCCTACGTCTTTATGTTTATCGAAGCGATGGC
CGACGCTGCCGTGCTGGGCGGGATGCCACGCGCCCAGGCGTATAAATTTGCCGCTCAGGCGGTAATGGGTTCCGCAAAAA
TGGTGCTGGAAACGGGAGAACATCCGGGGGCACTGAAAGATATGGTCTGCTCACCGGGAGGCACCACCATTGAGGCGGTA
CGCGTACTGGAAGAGAAAGGCTTCCGTGCCGCAGTGATCGAAGCGATGACGAAGTGTATGGAAAAATCAGAAAAACTCAG
TAAATCCTGA

Upstream 100 bases:

>100_bases
TAATCCTCTATTGTGTCGCGCTTTTGCCTTCCGGCATAGTTCTGTTTATGCTTCTGCCAGCGATTATCAAAACAATGAAT
TTCACGGCAGGAGTGAGGCA

Downstream 100 bases:

>100_bases
TGACTTTTGCCGGACGTCAGGCCGCCACTTCGGTGCGGTTACGTCCGGCTTTCTTTGCTTTGTAAAGCGCCAAATCTGCC
GATTTCAACCACTCACGATA

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS

Sequences:

>Translated_269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
>Mature_269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI24797097, Length=266, Percent_Identity=37.593984962406, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI24797095, Length=266, Percent_Identity=37.593984962406, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI21361454, Length=266, Percent_Identity=37.593984962406, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI198041662, Length=266, Percent_Identity=34.2105263157895, Blast_Score=167, Evalue=9e-42,
Organism=Escherichia coli, GI1786585, Length=269, Percent_Identity=99.6282527881041, Blast_Score=545, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17569021, Length=264, Percent_Identity=40.530303030303, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17540664, Length=285, Percent_Identity=36.8421052631579, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320861, Length=272, Percent_Identity=29.0441176470588, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21358587, Length=265, Percent_Identity=39.622641509434, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24648116, Length=277, Percent_Identity=38.2671480144404, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24647700, Length=169, Percent_Identity=42.0118343195266, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 28159; Mature: 28159

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEV
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHH
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCC
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCHHHHH
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEV
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHH
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITLDQLARALGHDRKIIRAMP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCC
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCHHHHH
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6296787; 9278503 [H]