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The map label for this gene is ddl [H]

Identifier: 218703662

GI number: 218703662

Start: 455194

End: 456288

Strand: Reverse

Name: ddl [H]

Synonym: ECUMN_0418

Alternate gene names: 218703662

Gene position: 456288-455194 (Counterclockwise)

Preceding gene: 218703668

Following gene: 218703660

Centisome position: 8.77

GC content: 51.23

Gene sequence:

>1095_bases
ATGGAAAAACTGCGGGTAGGAATCGTTTTTGGTGGTAAATCAGCGGAACATGAAGTGTCTCTGCAATCGGCAAAAAACAT
TGTCGATGCCATTGATAAAAGTCGCTTCGACGTTGTGCTGCTGGGCATTGATAAACAAGGGCAATGGCACGTCAGCGATG
CCAGCAATTATCTGCTAAATGCAGACGATCCTGCCCATATTGCATTGCGCCCTTCGGCGACCAGTCTTGCGCAGGTGCCT
GGTAAACATGAACATCAGCTTATCGACACGCAAAACGGTCAGCCGTTGCCGACGGTGGATGTCATTTTCCCGATTGTCCA
CGGTACGCTGGGCGAAGATGGTTCTTTGCAGGGAATGCTGCGGGTCGCCAATTTACCGTTTGTAGGTTCTGATGTTCTGG
CTTCAGCGGCCTGTATGGATAAAGATGTCACCAAACGTCTGCTGCGCGATGCCGGGCTGAACATTGCGCCATTTATTACC
CTGACGCGCGCTAATCGTCACAACATCAGTTTTGCCGAAGTGGAGTCTAAACTGGGGTTACCGCTGTTTGTAAAACCGGC
TAATCAGGGCTCTTCTGTTGGTGTCAGCAAAGTAACCAGTGAAGAACAGTACGCAATTGCCGTCGATCTGGCGTTCGAGT
TCGACCATAAAGTGATCGTTGAGCAAGGGATCAAAGGTCGTGAGATCGAATGCGCAGTTCTGGGCAACGACAATCCGCAA
GCCAGCACCTGTGGCGAGATCGTACTCACCAGCGATTTCTATGCCTACGACACCAAGTACATTGATGAAGATGGCGCGAA
AGTGGTAGTTCCGGCAGCCATTGCGCCAGAAATCAACGATAAGATCCGGGCGATTGCCGTTCAGGCTTATCAGACGTTGG
GATGCGCAGGCATGGCGCGTGTAGACGTGTTTTTAACCCCAGAGAACGAAGTGGTGATCAACGAGATCAACACACTGCCT
GGCTTCACCAACATCAGTATGTATCCGAAGCTGTGGCAAGCCAGCGGTCTGGGTTACACCGACCTGATCACCCGTTTGAT
TGAACTGGCGCTGGAGCGTCACGCTGCGGATAACGCATTGAAAACCACAATGTAA

Upstream 100 bases:

>100_bases
TTGTCAGCAGAAGATTGCAACATTCTGTCAGAAGGCCGCGAATCCCCCGTTTTGTCGTGGTAATGTATGCACTTTGTGTT
AGACGGGATAGGTTTTTAAG

Downstream 100 bases:

>100_bases
TTTTTAAATGCCTGATAACTTTTATTGGGCATTTAAGACTGATGGAAAAAGTAAGATTGCCTGATGCGCTTTGCTTATCA
GGCCTACCAGATAATTGCAA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVP
GKHEHQLIDTQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLP
GFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM

Sequences:

>Translated_364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVP
GKHEHQLIDTQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLP
GFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM
>Mature_364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVP
GKHEHQLIDTQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLP
GFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=364, Percent_Identity=99.7252747252747, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1786280, Length=253, Percent_Identity=39.1304347826087, Blast_Score=181, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39346; Mature: 39346

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLN
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCEEEC
ADDPAHIALRPSATSLAQVPGKHEHQLIDTQNGQPLPTVDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
RVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFITLTRANRHNISFAEVESKLGL
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHCCC
PLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
EEEEEECCCCCCCCCHHCCCCCCEEEEEEEEEECCCEEEECCCCCCCEEEEEEECCCCCC
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMAR
CCCCCCEEEECCEEEECCEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEE
VDVFLTPENEVVINEINTLPGFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNAL
EEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
KTTM
HCCC
>Mature Secondary Structure
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLN
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCEEEC
ADDPAHIALRPSATSLAQVPGKHEHQLIDTQNGQPLPTVDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
RVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFITLTRANRHNISFAEVESKLGL
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHCCC
PLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
EEEEEECCCCCCCCCHHCCCCCCEEEEEEEEEECCCEEEECCCCCCCEEEEEEECCCCCC
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMAR
CCCCCCEEEECCEEEECCEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEE
VDVFLTPENEVVINEINTLPGFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNAL
EEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHH
KTTM
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]