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The map label for this gene is cynR [H]

Identifier: 218703625

GI number: 218703625

Start: 416167

End: 417066

Strand: Reverse

Name: cynR [H]

Synonym: ECUMN_0381

Alternate gene names: 218703625

Gene position: 417066-416167 (Counterclockwise)

Preceding gene: 218703629

Following gene: 218703618

Centisome position: 8.02

GC content: 54.56

Gene sequence:

>900_bases
ATGCTCTCTCGACATATCAATTATTTTCTTGCCGTGGCTGAACATGGCAGCTTTACCCGTGCCGCCAGTGCGTTGCACGT
CTCCCAGCCTGCGCTTTCCCAGCAGATTCGCCAGTTAGAGGAGAGTTTAGGCGTGCCGCTGTTTGACCGTAGCGGGCGAA
CGATTCGTCTCACTGATGCAGGAGAAGTCTGGCGACAGTACGCCAGCCGGGCGTTACAGGAACTGGGGGCGGGTAAACGG
GCGATTCATGATGTTGCCGATCTGACGCGAGGATCGCTGCGTATCGCCGTCACCCCCACCTTTACGAGCTACTTTATCGG
CCCTTTAATGGCGGATTTCTATGCGCGCTACCCCGGCATCACGCTCAAGCTACAGGAAATGTCGCAGGAGAAAATCGAAG
ATCTGCTTTGTCGCGACGAGTTGGACGTTGGGATTGCCTTCGCGCCTGTGCATTCGCCGGAGCTGGAGGCAATTCCTTTA
CTGACAGAAAGTTTAGCGTTAGTCGTGGCGCAACATCATCCGCTGGCTGCCTGTGAACAGGTGGCGTTGAGTCGCTTGCA
TGATGAAAAACTGGTCCTGCTCAGCGCGGAATTTGCCACCAGAGAGCAAATTGACCACTACTGTGAGAAAGCGGGGCTAC
ATCCACAGGTGGTCATTGAGGCGAACTCAATTAGCGCGGTTCTGGAGCTGATTCGCCGCACTTCCCTTTCCACATTGTTG
CCAGCAGCGATTGCCACACAACATGAAGGGCTTAAAGCTATTTCTCTTGCCCCACCACTACTGAAGAGAACGGCGGTTTT
GTTGCGGCGGAAAAATAGCTGGCAGACAGCCGCCGCGAAGGCATTTTTGCAAATGGCGTTGGATAAATGCGCGGTTGTTG
GCGGGAATGAATCGCGGTAA

Upstream 100 bases:

>100_bases
CTGTCTGTCTCTGGAATGAGAGGCAGACTACTCGCCGATTGTCATAAGGTAAAAGTCTCATTTATGATGAGTTCTATTGG
ATTTACTTATAGGTTGGCGA

Downstream 100 bases:

>100_bases
CCACTGACACAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGTTCAGTGCTCTACGTAGGCCGGATAAGGCGTTCA
CGCCGCATCCGGCATAAACT

Product: DNA-binding transcriptional regulator CynR

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLKLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHEGLKAISLAPPLLKRTAVLLRRKNSWQTAAAKAFLQMALDKCAVVGGNESR

Sequences:

>Translated_299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLKLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHEGLKAISLAPPLLKRTAVLLRRKNSWQTAAAKAFLQMALDKCAVVGGNESR
>Mature_299_residues
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKR
AIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGITLKLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
PAAIATQHEGLKAISLAPPLLKRTAVLLRRKNSWQTAAAKAFLQMALDKCAVVGGNESR

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=299, Percent_Identity=97.3244147157191, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1790399, Length=276, Percent_Identity=30.4347826086957, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=29.1095890410959, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693105, Length=284, Percent_Identity=28.169014084507, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788748, Length=303, Percent_Identity=27.7227722772277, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787530, Length=235, Percent_Identity=29.7872340425532, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081904, Length=227, Percent_Identity=33.0396475770925, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787601, Length=294, Percent_Identity=24.8299319727891, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=30.6451612903226, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI2367136, Length=227, Percent_Identity=31.2775330396476, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786401, Length=171, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789173, Length=212, Percent_Identity=31.6037735849057, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789204, Length=291, Percent_Identity=27.4914089347079, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=28.8194444444444, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790208, Length=182, Percent_Identity=31.8681318681319, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788296, Length=213, Percent_Identity=28.6384976525822, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=24.9122807017544, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790262, Length=236, Percent_Identity=25, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789639, Length=142, Percent_Identity=33.8028169014084, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786448, Length=158, Percent_Identity=34.1772151898734, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=26.6211604095563, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787949, Length=168, Percent_Identity=32.7380952380952, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=160, Percent_Identity=30.625, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=219, Percent_Identity=27.8538812785388, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787589, Length=116, Percent_Identity=33.6206896551724, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788706, Length=172, Percent_Identity=32.5581395348837, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081684, Length=285, Percent_Identity=23.5087719298246, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786713, Length=175, Percent_Identity=29.1428571428571, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082184, Length=252, Percent_Identity=25, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32855; Mature: 32855

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDA
CCCCHHHHEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECH
GEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
TLKLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPLLTESLALVVAQHHPLAACEQ
EEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PAAIATQHEGLKAISLAPPLLKRTAVLLRRKNSWQTAAAKAFLQMALDKCAVVGGNESR
HHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLGVPLFDRSGRTIRLTDA
CCCCHHHHEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECH
GEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFTSYFIGPLMADFYARYPGI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
TLKLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPLLTESLALVVAQHHPLAACEQ
EEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVVIEANSISAVLELIRRTSLSTLL
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PAAIATQHEGLKAISLAPPLLKRTAVLLRRKNSWQTAAAKAFLQMALDKCAVVGGNESR
HHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]