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The map label for this gene is prpR [H]

Identifier: 218703618

GI number: 218703618

Start: 404773

End: 406359

Strand: Reverse

Name: prpR [H]

Synonym: ECUMN_0374

Alternate gene names: 218703618

Gene position: 406359-404773 (Counterclockwise)

Preceding gene: 218703625

Following gene: 218703602

Centisome position: 7.81

GC content: 55.01

Gene sequence:

>1587_bases
ATGGCACATCCACCACGGCTGAATGACGACAAACCGGTTATCTGGACGGTATCTGTAACGCGCCTGTTCGAGCTGTTTCG
CGATATCAGCCTCGAGTTTGATCATCTGGCGAACATTACCCCTATTCAGCTTGGCTTTGAAAAAGCAGTGACCTACATCC
GCAAGAAACTGGCAAACGAACGCTGCGACGCCATCATCGCCGCTGGCTCTAACGGCGCGTACCTGAAAAGCCGCCTGTCA
GTGCCAGTTATTTTGATTAAACCGAGCGGCTACGATGTGTTACAGGCACTGGCAAAAGCCGGAAAACTCACCTCTTCTAT
CGGCGTTGTCACTTATCAGGAAACTATTCCGGCACTGGTGGCGTTTCAAAAAACCTTTAATTTGCGCCTCGACCAACGTA
GCTACATTACCGAAGAAGACGCACGCGGGCAGATTAACGAGCTGAAAGCTAACGGCACCGAAGCGGTGGTCGGCGCGGGG
CTAATTACCGATCTGGCAGAAGAAGCCGGAATGACCGGAATTTTTATCTATTCCGCCGCCACCGTGCGCCAGGCATTCAG
CGATGCGCTGGATATGACGCGCATGTCGTTACGCCATAACACTCACGATGCCACCCGCAACGCCCTGCGTACTCGTTACG
TGCTGGGCGATATGCTCGGTCAATCACCACAGATGGAACAGGTACGGCAGACTATTTTGCTGTATGCCCGCTCCAGTGCA
GCGGTGTTGATTGAAGGGGAAACGGGGACGGGCAAAGAGCTGGCGGCCCAGGCGATTCATCGGGAATATTTTGCCCGCCA
CGATGCGCGACAGGGCAAAAAGTCGCATCCGTTTGTTGCCGTCAACTGCGGAGCGATTGCCGAATCGCTGCTGGAAGCGG
AGCTGTTTGGCTATGAGGAAGGGGCGTTTACCGGCTCGCGACGCGGAGGTCGCGCCGGGCTGTTCGAAATTGCCCACGGC
GGTACGCTGTTTCTGGATGAGATTGGCGAAATGCCGCTACCTTTGCAGACTCGCCTGTTACGGGTGCTGGAAGAAAAAGA
GGTCACCCGCGTCGGCGGGCATCAGCCTGTTCCGGTGGATGTGCGAGTCATTAGCGCCACTCACTGCAATCTGGAAGAAG
ATATGCGGCAAGGGCAGTTTCGCCGTGACCTGTTTTATCGGCTGAGCATTTTACGCCTGCAACTGCCACCACTGCGTGAG
CGGGTGGCAGATATTCTGCCGCTGGCGGAAAGCTTTTTGAAAGTGTCTCTGGCTGCACTTGCTGCCCCGTTTTCTTCCGC
ATTACGCCAGGGATTACAGGCAAGCGAAACCGTGCTGGTGCACTACGACTGGCCGGGCAATATTCGAGAACTGCGCAATA
TGATGGAGCGACTGGCGCTGTTTTTAAGTGTGGAACCGACGCCGGATTTAACGCCGCAGTTTTTGCAGCTGCTACTGCCG
GAACTAGCGCGCGAATCAGCGAAAATTCCCGCTCCACGCTTACTGACACCACAACAGGCACTGGAGAAATTTAAAGGCGA
TAAAACAGCAGCGGCGAATTATTTAGGTATCAGCCGGACGACGTTCTGGCGGCGGCTGAAAAACTGA

Upstream 100 bases:

>100_bases
TTTCAGTTAACGTTTCAGTCAATGTTTCACCGCGTTTCATTGCAACAATTATGAAACAAGACTAAACCCAATATTCGGTT
TCTTAACTTTGCGGTGCGCT

Downstream 100 bases:

>100_bases
ACTGCCGAATGCAGCGCGAACGCTTTATCCGGCATAAATTTCAATAGATGATGATCACCGATAAACTTACGCATCGGGGA
TGTTTACATAGGTTTCAGAA

Product: DNA-binding transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLS
VPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
AVLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHG
GTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRE
RVADILPLAESFLKVSLAALAAPFSSALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
ELARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN

Sequences:

>Translated_528_residues
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLS
VPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
AVLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHG
GTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRE
RVADILPLAESFLKVSLAALAAPFSSALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
ELARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN
>Mature_527_residues
AHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSV
PVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGL
ITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSAA
VLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGG
TLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRER
VADILPLAESFLKVSLAALAAPFSSALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLPE
LARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN

Specific function: Involved in the transcriptional regulation of the propionate catabolism operon [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1786524, Length=528, Percent_Identity=98.2954545454545, Blast_Score=1063, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=338, Percent_Identity=42.8994082840237, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1790299, Length=345, Percent_Identity=42.8985507246377, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790437, Length=325, Percent_Identity=43.6923076923077, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI87082117, Length=355, Percent_Identity=42.2535211267606, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=38.2978723404255, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1788905, Length=378, Percent_Identity=39.4179894179894, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789087, Length=326, Percent_Identity=37.1165644171779, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082152, Length=246, Percent_Identity=45.5284552845528, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=241, Percent_Identity=43.5684647302905, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787583, Length=237, Percent_Identity=44.3037974683544, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=33.993399339934, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=35.5648535564854, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR012704
- InterPro:   IPR010524 [H]

Pfam domain/function: PF02954 HTH_8; PF06506 PrpR_N; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58671; Mature: 58540

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANE
CCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALV
HCCEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHCCEEEEHHHHHHHH
AFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGLITDLAEEAGMTGIFIYSAA
HHHHHHCCEECCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHH
TVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
AVLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVD
CCCCCCCCCCCCCEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRERVADILPLAESFLKVSLAAL
EEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAPFSSALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
ELARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN
HHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
AHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQLGFEKAVTYIRKKLANE
CCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGKLTSSIGVVTYQETIPALV
HCCEEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHHHHCCEEEEHHHHHHHH
AFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAGLITDLAEEAGMTGIFIYSAA
HHHHHHCCEECCHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHH
TVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDMLGQSPQMEQVRQTILLYARSSA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
AVLIEGETGTGKELAAQAIHREYFARHDARQGKKSHPFVAVNCGAIAESLLEAELFGYEE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKEVTRVGGHQPVPVD
CCCCCCCCCCCCCEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VRVISATHCNLEEDMRQGQFRRDLFYRLSILRLQLPPLRERVADILPLAESFLKVSLAAL
EEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAPFSSALRQGLQASETVLVHYDWPGNIRELRNMMERLALFLSVEPTPDLTPQFLQLLLP
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
ELARESAKIPAPRLLTPQQALEKFKGDKTAAANYLGISRTTFWRRLKN
HHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]