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The map label for this gene is insO2 [H]

Identifier: 218703500

GI number: 218703500

Start: 282403

End: 283449

Strand: Reverse

Name: insO2 [H]

Synonym: ECUMN_0248

Alternate gene names: 218703500

Gene position: 283449-282403 (Counterclockwise)

Preceding gene: 218703501

Following gene: 218703497

Centisome position: 5.45

GC content: 53.68

Gene sequence:

>1047_bases
TTGGGTGCGCCAGCTCAGGCGAGAGCGTCAGGGGATTGCGCCCTCTGCCACACCTATTACTCCAGACCAGCAACGTATCC
GCGAACTGGAAAAGCAGGTTCGCCGCCTGGAGGAACAAAATACGATATTAAAAAAGGCTACCGCGCTCTTGATGTCCGAC
TCGCTGAACGGTTCACGATAGTTGCCAGACTGAGTGACAGCCACTCAGTTGTCAGCCTCTGTTCTGCTCTGGAAATACAC
CGCAGCAGTTACCGGTACTGGCGAAAACGACGCGATACGGTTAATCCGGCGCGAGTCAGGTTGTGCAGCGAAATACGCCG
GGCGTGGAACCAAAGTAGGGGCTCTGCGGGGGCGCGCACGCTGGCTGAAATGCTGACCCAAAACGGCGTCCCGATGAGCC
GTTACCGTGCCGGGCGTCTGATGAAATATCTGAACCTGAGCAGTTGTCAGCCCGGAAAACATCAGTACAAAAATGCTCGT
CAGGAACATACCTGTCTGCCGAATCTGCTTGAGCGCCAGTTCGCTGTGCCCGAGCCAGATAGGGTATGGTGCGGAGATAT
TACGTATATCTGGGCAGGAAATCGCTGGTGCTATCTGGCGGTCGTCATGGATCTTTTTGCCCGCAGGGTTATCGGCTGGA
GCCTGTCAGCGAATGCCGATACTGCCCTGATAAGCAGTGCCCTGCGGATGGCGTATGAGGTGCGTGGTCAGCCGCGGGAC
GTCATGTTCCATAGCGACCAGGGAAGTCAATATACAGGACTGAAATATCAACAACTTCTCTGGCGTTACAGGATAAAGCA
AAGTGTCAGCCGACGGGGAAACTGCTGGGATAACAGTCCAATGGAACGCTTCTTCCGCAGTCTGAAAACAGAATGGGTGC
CAACCGATGGCTACACAGGCAAAGACGTGGCCCGGCAGCAAATCAGCAGTTATATTCTGAATTATTACAATAGCGTCAGG
CCTCACCATTATAACGGAGGGCTGACGCCGGAAGAGTCAGAGAACAGATACCATTTTTACTGTAAAACCGTGGCCAGTAT
TACTTGA

Upstream 100 bases:

>100_bases
CTGAATTCAGGCTGGAATGTGCACAGCTAATTGTTGATAAGGGCTACTCATATCGACAAGCCAGTGAAGCGATGAATGTC
GGTTCTACCACGCTTGAGAG

Downstream 100 bases:

>100_bases
CCACTACATGTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCACCGGTGCACAATTGCCGGATGCGGTGTGAACGCC
TTATCCGGCCCACCGATCCG

Product: transposase ORF B, IS3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MGAPAQARASGDCALCHTYYSRPATYPRTGKAGSPPGGTKYDIKKGYRALDVRLAERFTIVARLSDSHSVVSLCSALEIH
RSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNAR
QEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRD
VMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVR
PHHYNGGLTPEESENRYHFYCKTVASIT

Sequences:

>Translated_348_residues
MGAPAQARASGDCALCHTYYSRPATYPRTGKAGSPPGGTKYDIKKGYRALDVRLAERFTIVARLSDSHSVVSLCSALEIH
RSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNAR
QEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRD
VMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVR
PHHYNGGLTPEESENRYHFYCKTVASIT
>Mature_347_residues
GAPAQARASGDCALCHTYYSRPATYPRTGKAGSPPGGTKYDIKKGYRALDVRLAERFTIVARLSDSHSVVSLCSALEIHR
SSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQ
EHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDV
MFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRP
HHYNGGLTPEESENRYHFYCKTVASIT

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=254, Percent_Identity=32.2834645669291, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1788405, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1787263, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1786753, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1786570, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1786490, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1789981, Length=281, Percent_Identity=33.0960854092527, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 39990; Mature: 39859

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAPAQARASGDCALCHTYYSRPATYPRTGKAGSPPGGTKYDIKKGYRALDVRLAERFTI
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHEEE
VARLSDSHSVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGART
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
LAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPD
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRD
CEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
VMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTG
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
KDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVASIT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHHCC
>Mature Secondary Structure 
GAPAQARASGDCALCHTYYSRPATYPRTGKAGSPPGGTKYDIKKGYRALDVRLAERFTI
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHEEE
VARLSDSHSVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGART
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
LAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPD
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRD
CEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
VMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTG
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
KDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVASIT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]