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The map label for this gene is yafK

Identifier: 218703497

GI number: 218703497

Start: 279780

End: 280520

Strand: Reverse

Name: yafK

Synonym: ECUMN_0245

Alternate gene names: 218703497

Gene position: 280520-279780 (Counterclockwise)

Preceding gene: 218703500

Following gene: 218703494

Centisome position: 5.39

GC content: 48.99

Gene sequence:

>741_bases
ATGCGTAAAATCGCATTAATTCTTGCGATGTTGTTGATCCCGTGCGTTTCGTTTGCCGGTTTGCTGGGCAGCAGTAGCTC
GACCACGCCAGTGAGCAAAGAGTATAAGCAGCAGTTGATGGGATCCCCTGTCTACATCCAGATCTTCAAGGAAGAACGTA
CGCTCGATCTCTACGTCAAAATGGGCGAGCAATATCAACTGCTCGACAGCTATAAAATCTGTAAATATTCCGGCGGCTTA
GGACCAAAACAGCGTCAGGGCGATTTCAAAAGCCCGGAAGGGTTTTATAGCGTCCAGCGTAATCAGTTAAAACCAGACAG
CCGTTACTACAAAGCGATTAATATTGGATTCCCCAATGCCTATGACCGTGCGCATGGTTATGAAGGGAAATACCTGATGA
TCCACGGCGATTGTGTTTCCATCGGCTGCTACGCGATGACCAATCAGGGTATTGATGAGATCTTCCAGTTCGTTACTGGT
GCGCTGGTGTTTGGTCAGCCGAGCGTGCAAGTGAGTATTTACCCGTTCCGTATGACCGACGCTAATATGAAGCGCCATAA
GTATTCCAACTTTAAGGACTTCTGGGAGCAACTGAAACCGGGCTACGACTACTTTGAGCAAACCCGTAAGCCACCGACCG
TTTCTGTGGTCAATGGCCGTTACGTGGTCAGCAAGCCGTTAAGCCACGAAGTTGTGCAACCACAGCTGGCATCAAACTAC
ACGCTCCCCGAGGCAAAATAA

Upstream 100 bases:

>100_bases
TTTTGGTGATAATGTGAGCCTTCCAAATCCAGACTATTCCGATTTGGGATCCATTTTGCCGTCCTGGCGTGTGTAACCGT
TTTATCAAGGAATAAGCAGT

Downstream 100 bases:

>100_bases
GCGCCATTCGCCTGGCATAATCTTTTGCCAGGTTTCATTGCCCGTCAGCGGCTGTGTCGCAATCACCGTGACCACATCAT
TAGGTGTGGTCTGCGAGCTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPVYIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGL
GPKQRQGDFKSPEGFYSVQRNQLKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVNGRYVVSKPLSHEVVQPQLASNY
TLPEAK

Sequences:

>Translated_246_residues
MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPVYIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGL
GPKQRQGDFKSPEGFYSVQRNQLKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVNGRYVVSKPLSHEVVQPQLASNY
TLPEAK
>Mature_246_residues
MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPVYIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGL
GPKQRQGDFKSPEGFYSVQRNQLKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVNGRYVVSKPLSHEVVQPQLASNY
TLPEAK

Specific function: Unknown

COG id: COG3034

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family

Homologues:

Organism=Escherichia coli, GI1786418, Length=246, Percent_Identity=100, Blast_Score=511, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YAFK_ECO57 (P0AAA0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85511
- PIR:   C90660
- RefSeq:   NP_285941.1
- RefSeq:   NP_308278.1
- ProteinModelPortal:   P0AAA0
- EnsemblBacteria:   EBESCT00000028848
- EnsemblBacteria:   EBESCT00000057445
- GeneID:   914338
- GeneID:   957037
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0282
- KEGG:   ecs:ECs0251
- GeneTree:   EBGT00050000010938
- HOGENOM:   HBG416623
- OMA:   LMIHGEC
- ProtClustDB:   CLSK879594
- BioCyc:   ECOL83334:ECS0251-MONOMER
- InterPro:   IPR005490

Pfam domain/function: PF03734 YkuD

EC number: NA

Molecular weight: Translated: 28022; Mature: 28022

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPVYIQIFKEERTLDLYVK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEEEEEE
MGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQLKPDSRYYKAINIGFPNA
CCCCEEHHHHHCEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCC
YDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTGALVFGQPSVQVSIYPFRMTD
HHHCCCCCCCEEEEECCEEEEEEEECCCCCHHHHHHHHHCEEEECCCCEEEEEEEEEECC
ANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVNGRYVVSKPLSHEVVQPQLASNY
CCHHHHHCCCHHHHHHHHCCCHHHHHHCCCCCEEEEECCEEEEECCCCHHHCCCHHHCCC
TLPEAK
CCCCCC
>Mature Secondary Structure
MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPVYIQIFKEERTLDLYVK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEEEEEE
MGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQLKPDSRYYKAINIGFPNA
CCCCEEHHHHHCEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCC
YDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTGALVFGQPSVQVSIYPFRMTD
HHHCCCCCCCEEEEECCEEEEEEEECCCCCHHHHHHHHHCEEEECCCCEEEEEEEEEECC
ANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVNGRYVVSKPLSHEVVQPQLASNY
CCHHHHHCCCHHHHHHHHCCCHHHHHHCCCCCEEEEECCEEEEECCCCHHHCCCHHHCCC
TLPEAK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796