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The map label for this gene is metJ

Identifier: 218701357

GI number: 218701357

Start: 3184506

End: 3184961

Strand: Direct

Name: metJ

Synonym: ECIAI39_3056

Alternate gene names: 218701357

Gene position: 3184506-3184961 (Clockwise)

Preceding gene: 218701354

Following gene: 218701358

Centisome position: 62.05

GC content: 50.44

Gene sequence:

>456_bases
ATGCATTATCACGCCGACAGGTGCATTACACGATGTCACGGTAACGCCTGTACGGTAAACTATGCGGGTTTACGGTCAGT
ACCCACATTCACTGTGTGGTCTGGTCTCAATTTATTGACGAAGAGGATTAAGTATCTCATGGCTGAATGGAGCGGCGAAT
ATATCAGCCCATACGCTGAGCACGGCAAGAAGAGTGAACAAGTCAAAAAGATTACGGTTTCCATTCCTCTTAAGGTGTTA
AAAATCCTCACCGATGAACGCACGCGTCGTCAGGTGAACAACCTGCGTCACGCGACCAACAGCGAGCTGCTGTGCGAAGC
GTTTCTGCATGCCTTTACCGGGCAACCTTTGCCGGATGATGCCGATCTGCGTAAAGAGCGCAGCGACGAAATCCCGGAAG
CGGCAAAAGAGATCATGCGTGAGATGGGGATTAACCCGGAGACGTGGGAATACTAA

Upstream 100 bases:

>100_bases
GCACCAGAGTAAACATTGTGTTAATGGACGTCAATACATCTGGACATCTAAACTTCTTTGCGTATAGATTGAGCAAATCC
CAAATAGCCGTTAAAATTAT

Downstream 100 bases:

>100_bases
AGCGGAAAAGAGCGGCGTGGAGTTAACCAGCCGCTTTTTTCATACATGGACGTTTAACTATGAAAATCAGGCTACTGATC
CCCGGCCTGCTGGTTTCTGT

Product: transcriptional repressor protein MetJ

Products: NA

Alternate protein names: Met regulon regulatory protein metJ [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MHYHADRCITRCHGNACTVNYAGLRSVPTFTVWSGLNLLTKRIKYLMAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVL
KILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPETWEY

Sequences:

>Translated_151_residues
MHYHADRCITRCHGNACTVNYAGLRSVPTFTVWSGLNLLTKRIKYLMAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVL
KILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPETWEY
>Mature_151_residues
MHYHADRCITRCHGNACTVNYAGLRSVPTFTVWSGLNLLTKRIKYLMAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVL
KILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPETWEY

Specific function: This regulatory protein, when combined with SAM (S- adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis [H]

COG id: COG3060

COG function: function code KE; Transcriptional regulator of met regulon

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metJ family [H]

Homologues:

Organism=Escherichia coli, GI1790373, Length=105, Percent_Identity=100, Blast_Score=219, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002084
- InterPro:   IPR010985
- ProDom:   PD020365 [H]

Pfam domain/function: PF01340 MetJ [H]

EC number: NA

Molecular weight: Translated: 17405; Mature: 17405

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYHADRCITRCHGNACTVNYAGLRSVPTFTVWSGLNLLTKRIKYLMAEWSGEYISPYAE
CCCCHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
HGKKSEQVKKITVSIPLKVLKILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDD
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
ADLRKERSDEIPEAAKEIMREMGINPETWEY
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHYHADRCITRCHGNACTVNYAGLRSVPTFTVWSGLNLLTKRIKYLMAEWSGEYISPYAE
CCCCHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
HGKKSEQVKKITVSIPLKVLKILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDD
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
ADLRKERSDEIPEAAKEIMREMGINPETWEY
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA