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The map label for this gene is hcaD

Identifier: 218701052

GI number: 218701052

Start: 2845245

End: 2846447

Strand: Direct

Name: hcaD

Synonym: ECIAI39_2743

Alternate gene names: 218701052

Gene position: 2845245-2846447 (Clockwise)

Preceding gene: 218701051

Following gene: 218701053

Centisome position: 55.44

GC content: 54.61

Gene sequence:

>1203_bases
ATGAAAGAAAAAACGATCATTATTGTCGGTGGTGGGCAAGCGGCGGCAATGGCTGCGGCCTCGCTTCGCCAGCAAGGGTT
CACCGGTGAGCTGCATCTGTTTTCCGATGAGCAACATCTTCCTTATGAACGCCCCCCGCTCTCGAAATCCATGTTGCTGG
AAGATTCCCCACAGTTGCAGTCTGTGTTACCCGCTCACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACC
ATCAAAACATTGGGCCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGC
AACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGACGCACTGGGAGAACGCTGCTTTACCCTGCGCCATGCCGGCGATG
CCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTGTCGGTGCCGGAACTATTGGTCTGGAACTGGCT
GCCAGCGCCACGCAGCGCGGATGTAAGGCGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCC
CGTACAACGCTATCTTTTACAGCGCCATCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTTGTCG
ATGGTGAAAACGTAGAACTGACGCTGCAAAGTGGCGAAACGCTTCGGGCCGATGTGGTGATTTACGGTATTGGTATCAGC
GCCAACGACCAACTGGCTCGCGAGGCCAACCTTGATACTGCCAATGGCATTGTCATTGATGAGGCTTGCCGCACCTGCGA
TCCCGCGATCTTTGCCGGTGGCGATGTGGCAATCACCCGTCTTGATAATGGTGCACTACACCGCTGCGAAAGCTGGGAAA
ACGCCAATAACCAGGCGCAAATTGCCGCTTCCGCAATGTTGGGGCTACCGCTTCCGCGACTGCCGCCGCCGTGGTTCTGG
AGCGATCAGTACAGTGATAACTTACAGTTTATTGGCGATATGCGTGGTGATGACTGGCTTTGTCGTGGCAACCCGGAAAC
TCAGAAGGCGATTTGGTTTAATCTGCAAAACGGCGTGCTTATCGGTGCGGTAACGCTGAATCAGGGGCGTGAGATTCGCT
TAATCCGCAAATGGATCCAGAGCGGCAAAACGTTTGATGCGAAACTGCTGACAGATGAGCACATCGCGCTTAAATCACTG
TAA

Upstream 100 bases:

>100_bases
TAATCGCGCATTAAGCGGTGTGATGATCAACGCTGATGCGGGTTTAGCGATTCGCGGCATTCGCCACGTAGCGGCTGGGC
TGGATCTTTAAGGAAGCACG

Downstream 100 bases:

>100_bases
CCAGGATAATTAGCGAATATCTCAATGCCTGGGGCGTGGCGAGGTGCCAGAGTGTGTATTACGTTTAAATCACATTATCT
TGCAAAGGGATTGGTTATGA

Product: phenylpropionate dioxygenase ferredoxin reductase subunit

Products: NA

Alternate protein names: Digoxigenin system ferredoxin--NAD(+) reductase component [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVT
IKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
ASATQRGCKATVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGENVELTLQSGETLRADVVIYGIGIS
ANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQIAASAMLGLPLPRLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLTDEHIALKSL

Sequences:

>Translated_400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVT
IKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
ASATQRGCKATVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGENVELTLQSGETLRADVVIYGIGIS
ANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQIAASAMLGLPLPRLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLTDEHIALKSL
>Mature_400_residues
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVT
IKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
ASATQRGCKATVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGENVELTLQSGETLRADVVIYGIGIS
ANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQIAASAMLGLPLPRLPPPWFW
SDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLTDEHIALKSL

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP- dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=393, Percent_Identity=27.735368956743, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI226437568, Length=393, Percent_Identity=27.735368956743, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI65787454, Length=393, Percent_Identity=27.735368956743, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=24.6290801186944, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=24.6290801186944, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=96.5, Blast_Score=725, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=274, Percent_Identity=28.4671532846715, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789765, Length=323, Percent_Identity=24.7678018575851, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=372, Percent_Identity=26.6129032258064, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24639257, Length=369, Percent_Identity=26.8292682926829, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI281359715, Length=369, Percent_Identity=26.8292682926829, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281359713, Length=369, Percent_Identity=26.8292682926829, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24639250, Length=369, Percent_Identity=26.8292682926829, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI18543267, Length=369, Percent_Identity=26.8292682926829, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24639252, Length=369, Percent_Identity=26.8292682926829, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24581020, Length=363, Percent_Identity=26.4462809917355, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28573993, Length=363, Percent_Identity=26.4462809917355, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585130, Length=326, Percent_Identity=23.3128834355828, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.18.1.3 [H]

Molecular weight: Translated: 43877; Mature: 43877

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQ
CCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEECCCCHHH
SVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDA
HHCCHHHCCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHH
LGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELAASATQRGCKATVIELAATVM
HHHHHEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGENVELTLQSGETLRADVVIYGIGIS
CCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCC
ANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQ
CCCHHHHHCCCCCCCCEEEECHHCCCCCCEEECCCEEEEEECCCHHHHHHCCCCCCCCHH
IAASAMLGLPLPRLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCEEEEEEECCCEE
IGAVTLNQGREIRLIRKWIQSGKTFDAKLLTDEHIALKSL
EEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCEEECCC
>Mature Secondary Structure
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYERPPLSKSMLLEDSPQLQ
CCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEECCCCHHH
SVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDA
HHCCHHHCCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHH
LGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELAASATQRGCKATVIELAATVM
HHHHHEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGENVELTLQSGETLRADVVIYGIGIS
CCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCC
ANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQ
CCCHHHHHCCCCCCCCEEEECHHCCCCCCEEECCCEEEEEECCCHHHHHHCCCCCCCCHH
IAASAMLGLPLPRLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCEEEEEEECCCEE
IGAVTLNQGREIRLIRKWIQSGKTFDAKLLTDEHIALKSL
EEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA