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The map label for this gene is hcaB

Identifier: 218701051

GI number: 218701051

Start: 2844423

End: 2845235

Strand: Direct

Name: hcaB

Synonym: ECIAI39_2742

Alternate gene names: 218701051

Gene position: 2844423-2845235 (Clockwise)

Preceding gene: 218701050

Following gene: 218701052

Centisome position: 55.42

GC content: 56.33

Gene sequence:

>813_bases
ATGAGCGATCTGCATAACGAGTCCATCTTTATTACCGGCGGCGGATCGGGATTAGGCCTGGCGCTGGTCGAGCGATTTAT
CGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCGAAAGTCGCCAGTCTGCGTCAGCGATTTGGTGAACATA
TTCTGGCGGTGGAAGGCAACGTGACCTGTTATGCCGATTATCAACGCGCGCTCGATCAGATCCTGACTCGTTCTGGCAAA
CTGGATTGTTTTATCGGCAATGCGGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAAC
CGGCTTCCACGAGCTGTTTAACGTCAACGTACTCGGTTACCTGCTGAGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCA
GTGAAGGCAGCATGATTTTCACACTGTCAAATGCCGCCTGGTATCCCGGCGGCGGTGGCCCGCTGTACACCGCCAGTAAA
CATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGTGTGG
TATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAA
TTGCCGCCATATTACCGCTGCAATTTTTCCCGCAACCGGCGGATTTTACGGGTCCGTATGTGATGTTGGCATCGCGGCGC
AATAATCGCGCATTAAGCGGTGTGATGATCAACGCTGATGCGGGTTTAGCGATTCGCGGCATTCGCCACGTAGCGGCTGG
GCTGGATCTTTAA

Upstream 100 bases:

>100_bases
CGGGGAAAGCATTATGCCTGCCCGCCACCGATCCGCTAACCACTTATCCAGTACACGTTGAAGGTGATGACATCTTCATC
GACTTACCGGAGGCGCAGCC

Downstream 100 bases:

>100_bases
GGAAGCACGATGAAAGAAAAAACGATCATTATTGTCGGTGGTGGGCAAGCGGCGGCAATGGCTGCGGCCTCGCTTCGCCA
GCAAGGGTTCACCGGTGAGC

Product: 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase

Products: NA

Alternate protein names: 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase; CI-dihydrodiol dehydrogenase; Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase; Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase; PP-dihydrodiol dehydrogenase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGNVTCYADYQRALDQILTRSGK
LDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGYLLSAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRR
NNRALSGVMINADAGLAIRGIRHVAAGLDL

Sequences:

>Translated_270_residues
MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGNVTCYADYQRALDQILTRSGK
LDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGYLLSAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRR
NNRALSGVMINADAGLAIRGIRHVAAGLDL
>Mature_269_residues
SDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGNVTCYADYQRALDQILTRSGKL
DCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGYLLSAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASKH
AATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRRN
NRALSGVMINADAGLAIRGIRHVAAGLDL

Specific function: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3- dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=234, Percent_Identity=29.9145299145299, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI40254992, Length=230, Percent_Identity=27.3913043478261, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI5031737, Length=194, Percent_Identity=29.8969072164948, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI33667109, Length=194, Percent_Identity=29.8969072164948, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI83715985, Length=175, Percent_Identity=30.8571428571429, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1788891, Length=270, Percent_Identity=98.8888888888889, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI1789208, Length=264, Percent_Identity=25, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI2367365, Length=243, Percent_Identity=28.8065843621399, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI72000259, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=271, Percent_Identity=26.9372693726937, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=277, Percent_Identity=23.4657039711191, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=25.6603773584906, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=23.9700374531835, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=263, Percent_Identity=27.7566539923954, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=188, Percent_Identity=28.1914893617021, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=203, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI23397609, Length=184, Percent_Identity=30.9782608695652, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17737361, Length=268, Percent_Identity=27.6119402985075, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24643142, Length=187, Percent_Identity=28.8770053475936, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.-

Molecular weight: Translated: 28514; Mature: 28383

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGN
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCEEEEEECC
VTCYADYQRALDQILTRSGKLDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGY
EEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHH
LLSAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASKHAATGLIRQLAYELAPKVRV
HHHHHHHCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
NGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRR
CCCCCCCCHHHCCCCHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
NNRALSGVMINADAGLAIRGIRHVAAGLDL
CCCEEEEEEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGN
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCEEEEEECC
VTCYADYQRALDQILTRSGKLDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGY
EEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHH
LLSAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASKHAATGLIRQLAYELAPKVRV
HHHHHHHCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
NGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRR
CCCCCCCCHHHCCCCHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
NNRALSGVMINADAGLAIRGIRHVAAGLDL
CCCEEEEEEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA