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The map label for this gene is hcaE

Identifier: 218701048

GI number: 218701048

Start: 2842230

End: 2843591

Strand: Direct

Name: hcaE

Synonym: ECIAI39_2739

Alternate gene names: 218701048

Gene position: 2842230-2843591 (Clockwise)

Preceding gene: 218701045

Following gene: 218701049

Centisome position: 55.38

GC content: 53.52

Gene sequence:

>1362_bases
ATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAACGGTCGGGTCACTCCGCGTATTTATACCGA
CCCGGACATTTACCAACTGGAGCTTGAGCGCATTTTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAA
AACCTGGTGATTTCTTTAACACCTACATGGGGGAAGATGCAGTTGTCGTGGTGCGCCAGAAAGACGGCAGCATCAAGGCG
TTTCTCAACCAGTGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGTCCGTA
TCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTA
AATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTGATTTTTGGCAACTGGGATACCAGCGCA
CCGGGCCTGCGTGATTACCTTGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAAT
TGTCGGCGGCGTACAAAAGTGGGTGATCAACTGCAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATG
CTCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGTCTCGGTGATGGACAA
ACTGCCCGCCCGGTGTGGGAAACCGCCAAAGATGCGCTGCAGTTTGGTCAGGACGGTCACGGCAGCGGTTTCTTCTTTAC
TGAAAAACCGGATGCTAATGTCTGGGTCGATGGCGCAGTTTCCAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAAC
GTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTCAACGGCACTGCC
ACGCTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTCGAAGTGTGGGCGTTCTGTATTACTGACAAAGCTGCCTCCGA
TGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGA
ACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACTGTGTCTGGAAATGGGGCTTGGTCAG
GAAAAGCACCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACGGCCGCTCGTGGAATGTACCAACG
CTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGTGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAAT
GA

Upstream 100 bases:

>100_bases
GGTAGAAATTAACTATTTCACATATCAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTT
ATCTTTCAGGATTAAAAAAT

Downstream 100 bases:

>100_bases
GTGCGCAAGTTTCACTGGAGTTACATCACCGCATTAGCCAGTTTCTCTTTCACGAAGCCAGTCTGCTGGATGACTGGAAA
TTTCGTGACTGGCTGGCACA

Product: 3-phenylpropionate dioxygenase large subunit alpha

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKA
FLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTA
TLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQ
EKHRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK

Sequences:

>Translated_453_residues
MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKA
FLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTA
TLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQ
EKHRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK
>Mature_452_residues
TTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAF
LNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSAP
GLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQT
ARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTAT
LRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQE
KHRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=453, Percent_Identity=99.7792494481236, Blast_Score=946, Evalue=0.0,
Organism=Escherichia coli, GI1788103, Length=154, Percent_Identity=27.9220779220779, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 51091; Mature: 50959

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFN
CCCCCCCEEEEEEECCCCEECCEEECCCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHHH
TYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELID
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCEEECCCCCCCCCCCCCEEE
VPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSAPGLRDYLGDIAWYLDGMLDR
CCCCCCCCHHHHHHHCCCCCCCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHC
REGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
CCCCHHHHCCCEEEEEECCCCCCHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRA
CCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHE
LRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRA
EEECCCCCCCCEEEECCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCC
FGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQEKHRDDGIPGITNYIFSETA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHH
ARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFN
CCCCCCEEEEEEECCCCEECCEEECCCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHHH
TYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELID
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCEEECCCCCCCCCCCCCEEE
VPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSAPGLRDYLGDIAWYLDGMLDR
CCCCCCCCHHHHHHHCCCCCCCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHC
REGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
CCCCHHHHCCCEEEEEECCCCCCHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRA
CCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHE
LRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRA
EEECCCCCCCCEEEECCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCC
FGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQEKHRDDGIPGITNYIFSETA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHH
ARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]